ReadyPrep™ Protein Extraction Kit (Membrane I)

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The ReadyPrep protein extraction kit (membrane I) uses temperature-dependent phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.

The ReadyPrep protein extraction kit (membrane I):

  • Isolates proteins associated with membranes or containing two transmembrane domains
  • Does not require the use of ultracentrifugation or density gradients
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
  • Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF

 

References
Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607.
Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.

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ReadyPrep™ Protein Extraction Kit (Membrane I)

1632088
50 preps, protein preparation and fractionation kit for most membrane proteins, temperature dependent differential solubilization with Triton X-114

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3103 Removal of Abundant Myofilament Proteins From Rabbit Myocardium Using the ReadyPrep Protein Extraction Kit (Membrane I) Click to download
4110148 Instruction Manual, ReadyPrep Protein Extraction Kit (Membrane I), Rev A Click to download