Description
Description
The ReadyPrep protein extraction kit (membrane I) uses temperature-dependent phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.
The ReadyPrep protein extraction kit (membrane I):
- Isolates proteins associated with membranes or containing two transmembrane domains
- Does not require the use of ultracentrifugation or density gradients
- Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
- Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF
References
Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607.
Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.
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50 preps, includes 50 ml membrane protein extraction buffer 1 (M1), 50 ml membrane protein extraction buffer 2 (M2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 25–50 mg of cells or tissue