ReadyPrep™ Protein Extraction Kit (Membrane I)


The ReadyPrep protein extraction kit (membrane I) uses temperature-dependent phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.

The ReadyPrep protein extraction kit (membrane I):

  • Isolates proteins associated with membranes or containing two transmembrane domains
  • Does not require the use of ultracentrifugation or density gradients
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
  • Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF


Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607.
Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.


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ReadyPrep™ Protein Extraction Kit (Membrane I), 50 preps
ReadyPrep™ Protein Extraction Kit (Membrane I), 50 preps

50 preps, includes 50 ml membrane protein extraction buffer 1 (M1), 50 ml membrane protein extraction buffer 1 (M1), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 25–50 mg of cells or tissue

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Number Description Options
3103 Removal of Abundant Myofilament Proteins From Rabbit Myocardium Using the ReadyPrep Protein Extraction Kit (Membrane I) Click to download
4110148 Instruction Manual, ReadyPrep Protein Extraction Kit (Membrane I), Rev A Click to download