The ReadyPrep™ 2-D starter kit is intended for first-time users of the PROTEAN® IEF cell and ReadyStrip™ IPG strips. The kit contains tested premixed reagents and a reference manual with technical tips, and it is designed to work with second-dimension cells.
The ReadyPrep 2-D starter kit includes all reagents needed to:
- Prepare an E. coli protein sample for IEF
- Rehydrate IPG strips with sample
- Equilibrate IPG strips for SDS-PAGE
- Overlay IPG strips with agarose on SDS-PAGE gels
Refer to the table below to order the appropriate strips and gels to use with the starter kit.
|2-D starter kit
|ReadyStrip IPG Strips, pH 4 – 7
|Tris-HCI gel, 8–16 % IPG well