Description
Description
The ReadyPrep™ 2-D starter kit is intended for first-time users of the PROTEAN® IEF cell and ReadyStrip™ IPG strips. The kit contains tested premixed reagents and a reference manual with technical tips, and it is designed to work with second-dimension cells.
The ReadyPrep 2-D starter kit includes all reagents needed to:
- Prepare an E. coli protein sample for IEF
- Rehydrate IPG strips with sample
- Equilibrate IPG strips for SDS-PAGE
- Overlay IPG strips with agarose on SDS-PAGE gels
Refer to the table below to order the appropriate strips and gels to use with the starter kit.
Strip Length (Compatible Cell) | |||
Accessory Product | 7 cm (Mini-PROTEAN® Tetra) | 11 cm (Criterion™) | 17 cm (PROTEAN II) |
2-D starter kit | 163-2105 | 163-2105 | 163-2105 |
ReadyStrip IPG Strips, pH 4 – 7 | 163-2001 | 163-2015 | 163-2008 |
Tris-HCI gel, 8–16 % IPG well | 161-1394 | — | — |
Ordering
Ordering
items
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(Discontinued) ReadyPrep™ 2-D Starter Kit
1632105
2-D gel electrophoresis kit, includes protein sample and buffers for IPG strip rehydration, focusing, and transfer to second dimension (IPG strips not included)
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(Discontinued) E. coli Protein Sample
1632110
2.7 mg, lyophilized control sample for use in IEF applications
Documents
Documents
Login Required Number Description Options
3095
2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B
2651
2-D Electrophoresis Workflow How-To Guide, Rev F
4110009
Instruction Manual, ReadyPrep 2-D Starter Kit, Rev A
2642
ReadyPrep 2-D Starter Kit Product Information Sheet