ReadyPrep™ 2-D Cleanup Kit

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ReadyPrep 2-D Cleanup Kit

Overview

SDS Removal

Before

After


Detergent removal using the ReadyPrep™ 2-D cleanup kit. E. coli extracts containing 1% SDS were electrophorised before and after treatment with the ReadyPrep 2-D cleanup kit. The samples were focused using 11 cm ReadyStrip™ pH 3–10 IPG strips, then run on Criterion™ 8–16 Tris-HCl precast gels for the second dimension.

Salt Removal

Salt removal using the ReadyPrep™ 2-D cleanup kit. E. coli extracts containing 1 M NaCl were electrophorised before and after treatment with the ReadyPrep 2-D cleanup kit. The samples were focused using 11 cm ReadyStrip™ pH 3–10 IPG strips, then run on Criterion™ 8–16 Tris-HCl precast gels for the second dimension.

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The ReadyPrep 2-D cleanup kit uses selective precipitation to remove ionic contaminants such as detergents, lipids, and phenolic compounds from protein samples. These contaminants may interfere with separation, particularly during IEF and 2-D separations. Use of the kit improves 2-D resolution and reproducibility. This kit can also be used to clean up samples prior to other applications in which contaminants may negatively impact results.

The ReadyPrep 2-D cleanup kit:

  • Employs a TCA-like protein precipitation to remove ionic contaminants from protein samples
  • Enables concentration of proteins from samples that are too dilute, allowing for higher protein loads that can improve spot detection
  • Can be used with virtually any protein sample
  • Reduces streaking, background staining, and other gel artifacts associated with ionic contaminants
  • Provides reagents for either 5 or 50 preparations
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ReadyPrep™ 2-D Cleanup Kit for IEF, 50 preps

1632130
50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein

0 1632130 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Cleanup/163-2130_0921120744196.xml 1632130 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US juso9ms8 2018-02-01 00:53:57 true true internet/product_SKU juso9ms8 templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Cleanup/163-2130_0921120744196.xml LSR 2019-05-04 16:23:45 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556909733780 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:45 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Cleanup/163-2130_0921120744196.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Sample-Cleanup/163-2130_0921120744196.xml 1632130-readyprep-2-d-cleanup-kit-for-ief-50-preps 1632130 ReadyPrep 2-D Cleanup Kit ReadyPrep&trade; 2-D Cleanup Kit for IEF, 50 preps 50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein 50 preps, general purpose protein preparation kit, includes precipitation agent unaffected by detergents, chaotropic agents, and other common reagents 50 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 50 x 100 µl protein samples each containing up to 500 µg protein /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2130_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2130_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2130_feature.jpg ReadyPrep&trade; 2-D Cleanup Kit for IEF, 50 preps preview ,VN RepEx 163-2130 50 preps, general purpose protein preparation kit, includes precipitation agent unaffected by detergents, chaotropic agents, and other common reagents 163-2130, 1632130, readyprep, 2-d, cleanup, 2d, 2de, gel, electrophoresis, ief, protein, sample, preparation, clean-up, precipitation, chaotropic, reagents 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Protein-Sample-Cleanup N 0 <p>Use the ReadyPrep 2-D Cleanup Kit to remove contaminants and concentrate protein samples for isoelectric focusing (IEF), 2-D gel electrophoresis, and any other application where protein sample cleanup or concentration is needed. The kit improves 2D resolution and reproducibility.</p> <ul> <li>Remove contaminants such as salts, lipids, detergents, nucleic acids and phenolic compounds</li> <li>Concentrate proteins in dilute samples</li> <li>Reduce streaking, background staining, and other artifacts</li> <li>Clean up protein samples in any form; precipitation is unaffected by the presence of chaotropes, detergents, common buffers, salts and other reagents commonly used in protein preparations</li> <li>Resuspend protein in any IEF/2DE-compatible or other rehydration/sample buffer</li> </ul> <p>The TCA-like precipitation removes ionic contaminants from samples. Proteins are quantitatively precipitated and concentrated, with substances that can interfere with IEF or other applications being washed away.&nbsp; Dilute samples can be concentrated for higher protein loads and enhanced spot detection. The isoelectric points of the proteins in the purified sample remain unaltered.</p> <h2 class="descHeader">Product Contents</h2> <p>For 50 samples, each 100 &micro;l containing up to 500 &micro;g protein</p> <ul> <li>Precipitation agent 1, 15 ml</li> <li>Precipitation agent 2, 15 ml</li> <li>Wash reagent 1, 2 ml</li> <li>Wash reagent 2, 50 ml</li> <li>Wash 2 additive, 0.25 ml</li> <li>Instruction manual</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="text-align: left;" valign="top"><strong>Number of Preps</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">5</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632140-readyprep-2-d-cleanup-kit-for-ief-5-preps?ID=1632140">1632140</a></td> </tr> <tr> <td style="text-align: left;" valign="top">50</td> <td style="text-align: center;" valign="top">1632130</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-cellular-location">Fractionation by Cellular Location</a></li> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a></li> <li>Aurum&trade; Ion Exchange Mini Kits for <a href="/en-us/category/fractionation-charge">Fractionation by Charge</a></li> <li>Rotofor<sup>&reg;</sup> Liquid-Phase IEF Cells for <a href="/en-us/category/fractionation-pi">Fractionation by pI</a></li> <li>Model 491 Prep Cell and Mini Prep Cell for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a> </li> <li><a href="/en-us/product/readyprep-2-d-starter-kit">ReadyPrep&trade; 2-D Starter Kit</a></li> <li><a href="/en-us/product/protean-i12-ief-system">PROTEAN<sup>&reg;</sup> i12&trade; IEF System</a></li> <li><a href="/en-us/product/readystrip-ipg-strips">ReadyStrip&trade; IPG Strips</a></li> <li>ReadyPrep Reduction-Alkylation Kit (<a href="/en-us/sku/1632090-readyprep-reduction-alkylation-kit-100-preps?ID=1632090">1632090</a>)</li> <li><a href="/en-us/product/powerpac-hv-high-voltage-power-supply">PowerPac&trade; HV High-Voltage Power Supply</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7-buffers-reagents">2-D Electrophoresis Buffers and Reagents</a></li> <li><a href="/en-us/product/ampholytes">Ampholytes</a></li> <li><a href="/en-us/category/tube-gel-ief-systems">Tube Gel IEF Systems</a></li> <li><a href="/en-us/category/imaging">Gel Imaging Systems</a></li> <li><a href="/en-us/product/2-d-electrophoresis-workflow">2-D Electrophoresis Workflow</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>ReadyPrep 2-D Cleanup Kit <a href="/webroot/web/pdf/lsr/literature/MS4110143A.pdf" target="_blank">Instruction Manual</a></li> <li>Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion <a href="/webroot/web/pdf/lsr/literature/Bulletin_3096.pdf" target="_blank">Brochure</a></li> <li>ReadyStrip IPG Strip <a href="/webroot/web/pdf/lsr/literature/4006166H.pdf" target="_blank">Instruction Manual</a></li> <li>PROTEAN i12 IEF System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf" target="_blank">Brochure</a></li> <li>Host Cell Protein (HCP) Workflow <a href="/webroot/web/pdf/lsr/literature/Bulletin_6493.pdf" target="_blank">Quick Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <p><a href="/en-us/applications-technologies/2-d-electrophoresis-analysis">2-D Electrophoresis and Analysis</a></p> /webroot/web/html/lsr/products/SKUs/autopopulated 1632130.html /webroot/web/html/lsr/products/SKUs/autopopulated/1632130.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632130&CartQuote=QT&quantity= 1432 true true false $345.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632130&AddToDefaultWishList=true&quantity= true false true 41115800 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632130&quantity= 1632130
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ReadyPrep™ 2-D Cleanup Kit for IEF, 5 preps

1632140
5 preps, includes 2 precipitation agents, 2 wash reagents, 1 wash reagent additive; for 5 x 100 µl protein samples each containing up to 500 µg protein

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The kit improves 2D resolution and reproducibility.</p> <ul> <li>Remove contaminants such as salts, lipids, detergents, nucleic acids and phenolic compounds</li> <li>Concentrate proteins in samples that are too dilute</li> <li>Reduce streaking, background staining, and other artifacts</li> <li>Cleanup not affected by the presence of chaotropes, detergents, common buffers, salts and other reagents commonly used in protein preparations</li> <li>Resuspend protein in any IEF/2D-compatible or other rehydration/sample buffer</li> </ul> <p>The TCA-like precipitation removes ionic contaminants from samples. Proteins are quantitatively precipitated and concentrated, with substances that can interfere with IEF or other applications being washed away.&nbsp; Dilute samples can be concentrated for higher protein loads and enhanced spot detection. There are no alterations in the isoelectric points of the proteins.</p> <h2 class="descHeader">Product Contents</h2> <p>For 5 samples, each 100 &micro;l containing up to 500 &micro;g protein</p> <ul> <li>Precipitation agent 1, 1.5 ml</li> <li>Precipitation agent 2, 1.5 ml</li> <li>Wash reagent 1, 0.2 ml</li> <li>Wash reagent 2, 5 ml</li> <li>Wash 2 additive, 0.25 ml</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" style="width: 300px;" border="0"> <tbody> <tr> <td style="text-align: left;" valign="top"><strong>Number of Preps</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">5</td> <td style="text-align: center;" valign="top">1632140</td> </tr> <tr> <td style="text-align: left;" valign="top">50</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632130-readyprep-2-d-cleanup-kit-for-ief-50-preps?ID=1632130">1632130</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-cellular-location">Fractionation by Cellular Location</a></li> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a></li> <li>Aurum&trade; Ion Exchange Mini Kits for <a href="/en-us/category/fractionation-charge">Fractionation by Charge</a></li> <li>Rotofor<sup>&reg;</sup> Liquid-Phase IEF Cells for <a href="/en-us/category/fractionation-pi">Fractionation by pI</a></li> <li>Model 491 Prep Cell and Mini Prep Cell for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a> </li> <li><a href="/en-us/product/readyprep-2-d-starter-kit">ReadyPrep&trade; 2-D Starter Kit</a></li> <li><a href="/en-us/product/protean-i12-ief-system">PROTEAN<sup>&reg;</sup> i12&trade; IEF System</a></li> <li><a href="/en-us/product/readystrip-ipg-strips">ReadyStrip&trade; IPG Strips</a></li> <li>ReadyPrep Reduction-Alkylation Kit (<a href="/en-us/sku/1632090-readyprep-reduction-alkylation-kit-100-preps?ID=1632090">1632090</a>)</li> <li><a href="/en-us/product/powerpac-hv-high-voltage-power-supply">PowerPac&trade; HV High-Voltage Power Supply</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7-buffers-reagents">2-D Electrophoresis Buffers and Reagents</a></li> <li><a href="/en-us/product/ampholytes">Ampholytes</a></li> <li><a href="/en-us/category/tube-gel-ief-systems">Tube Gel IEF Systems</a></li> <li><a href="/en-us/category/imaging">Gel Imaging Systems</a></li> <li><a href="/en-us/product/2-d-electrophoresis-workflow">2-D Electrophoresis Workflow</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>ReadyPrep 2-D Cleanup Kit <a href="/webroot/web/pdf/lsr/literature/MS4110143A.pdf" target="_blank">Instruction Manual</a></li> <li>Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion <a href="/webroot/web/pdf/lsr/literature/Bulletin_3096.pdf" target="_blank">Brochure</a></li> <li>ReadyStrip IPG Strip <a href="/webroot/web/pdf/lsr/literature/4006166H.pdf" target="_blank">Instruction Manual</a></li> <li>PROTEAN i12 IEF System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf" target="_blank">Brochure</a></li> <li>Host Cell Protein (HCP) Workflow <a href="/webroot/web/pdf/lsr/literature/Bulletin_6493.pdf" target="_blank">Quick Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <p><a href="/en-us/applications-technologies/2-d-electrophoresis-analysis">2-D Electrophoresis and Analysis</a></p> /webroot/web/html/lsr/products/SKUs/autopopulated 1632140.html /webroot/web/html/lsr/products/SKUs/autopopulated/1632140.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632140&CartQuote=QT&quantity= 1432 true true false $138.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632140&AddToDefaultWishList=true&quantity= true false true 41115800 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632140&quantity= 1632140
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Number Description Options
4110143 Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download

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