ReadyAgarose Precast Gels

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Overview

Specifications
  Mini Gels Wide Mini Gels
UV-transparent tray size (W x L) 7.1 x 10 cm 15.6 x 10 cm
Gel dimensions (W x L) 6.6 x 10 cm 15 x 10 cm
Compatible Sub-Cell models Mini-Sub cell GT Wide Mini-Sub cell GT
  Mini ReadySub-Cell GT Wide mini ReadySub-Cell GT
Comb configurations 8-well (20 µl) 20-well (20 µl)
  12-well (10 µl) 32-well (15 µl)*
    2 x 32-well (15 µl)*
    4 x 26 ReadyAgarose 96 Plus (15 µl)*
Buffers TAE or TBE TAE or TBE
Agarose percentage 1%, 3% 1%, 3%

* Multichannel pipet compatible.

Gel Selection Guide
Gel Percentage Optimal Resolution Range
  Mini and Wide Mini 96 Plus TAE 96 Plus TBE
1.0% 200 bp–10 kb 500 bp–10 kb 500 bp–10,000 kb
3.0%* 20–2,000 bp 200 bp–1,000 kb 50 bp–2,000 kb
* A 3% ReadyAgarose gel is easier to handle and gives the same performance as a standard 4% gel.
Migration From Lane to Lane

Reproducible migration from lane to lane in ReadyAgarose gels. A 3% gel was loaded with 375 bp and 530 bp fragments in alternating numbered lanes and with molecular marker, 100 bp–2 kb, in the marker lanes (M).

High Resolution ReadyAgarose 3% Gel

High resolution. ReadyAgarose 3% 12-well gel. Lanes 1, 2, 7, and 8, 20 bp EZ Load ruler; lanes 3, 4, 9, and 10, 100 bp EZ load molecular ruler; lanes 5, 6, 11, and 12, pBR322/EvaII and EcoRI digest. Gel was run at 100 V for 70 min in TAE buffer.

Agarose Gel Casting Guide

Hand Casting Options for a Variety of Gel Sizes

Two accessories are available for hand casting with the Sub-Cell family of electrophoresis cells: gel casters and casting gates. Gel casters offer the flexibility of accommodating several different-sized gel trays. These are available for the entire line of Sub-Cell systems. Casting gates, available in specific sizes for each cell, offer the convenience of casting directly in the Sub-Cell cells.



Gel Caster Guide
Tray Size (cm) Mini Gel Caster Full-Size Gel Caster Model 96 Gel Caster Model 192 Gel Caster
7 x 7
7 x 10
15 x 7
15 x 10
15 x 15  
15 x 20  
15 x 25  
25 x 10    
25 x 15    
25 x 20      
25 x 25      

Casting Guide
Electrophoresis Cell Tray Size (cm) Casting Gates Gel Caster
Mini-Sub cell GT 7 x 7
7 x 10  
Wide Mini-Sub cell GT 15 x 7
15 x 10  
Sub-Cell GT 15 x 10  
15 x 15
15 x 20  
15 x 25  
Sub-Cell Model 96 25 x 10
25 x 15  
Sub-Cell Model 192 25 x 10  
25 x 15
25 x 20  
25 x 25  

ReadyAgarose precast gels are prepared in UV-transparent gel trays designed to fit securely in Mini-Sub cell GT and wide Mini-Sub cell GT cells. ReadyAgarose gels come in a choice of 27 gel types, including ReadyAgarose 96 Plus gels, which resolve nucleic acids from 20 to 20,000 bp. The gels are individually packaged and cast in their own running tray using the Bio-Rad Certified line of agaroses. The UV-transparent trays have well numbers and a ruler printed on them for easy reference. ReadyAgarose precast gels will also fit other manufacturers' electrophoresis cells. Gel types to choose from include:

Mini ReadyAgarose Gels
Mini format precast gels fit securely in any of the Mini-Sub cell GT systems. For more details about optimal resolution, see the ReadyAgarose Gel Selection Guide.

  • 8-, 12-, or 2 x 8-well format
  • 1% and 3% agarose
  • TBE buffer with ethidium bromide
  • TAE buffer with or without ethidium bromide

 

Wide Mini ReadyAgarose Gels
Wide mini format precast gels fit securely in any of the wide Mini-Sub cell GT systems. For more details about optimal resolution, see the ReadyAgarose Gel Selection Guide.

  • 20-, 32-, 2 x 32-, or 4 x 26-well format
  • 1% and 3% agarose
  • TBE buffer with ethidium bromide
  • TAE buffer with ethidium bromide
  • Multichannel pipet compatible (32-, 2 x 32-, and 4 x 26-well)

 

ReadyAgarose 96 Plus Gels
ReadyAgarose 96 Plus precast gels are designed for high-throughput DNA electrophoretic applications, particularly for the analysis of PCR and RT-PCR products, DNA libraries, arrays, and SNPs, which often require running multiple samples on a single gel. The 2 cm resolution distance for high-definition separation of small fragments covers most high-throughput needs. These gels fit securely in any of the wide Mini-Sub cell GT systems.

  • 4 x 26-well format (96 wells and 8 reference lanes)
  • 1% and 3% agarose
  • TBE buffer with ethidium bromide
  • TAE buffer with ethidium bromide
  • Multichannel pipet compatible
  • ReadyAgarose 96 Plus wizard for data analysis

 

Mini ReadyAgarose Gel, TBE

161-3004
8-well precast mini gel, TBE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TBE

161-3006
8-well precast mini gel, TBE, 3.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TBE

161-3010
12-well precast mini gel, TBE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TBE

161-3012
12-well precast mini gel, TBE, 3.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3015
8-well precast mini gel, TAE, 1.0% agarose, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3016
8-well precast mini gel, TAE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3017
8-well precast mini gel, TAE, 3.0% agarose, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3018
8-well precast mini gel, TAE, 3.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3022
12-well precast mini gel, TAE, 1.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3024
12-well precast mini gel, TAE, 3.0% agarose plus ethidium bromide, 7.1 x 10 cm (tray size)

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Mini ReadyAgarose Gel, TAE

161-3057
2 x 8-well comb configuration, precast mini gel, TAE, 1.0% agarose, 7.1 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TBE

161-3028
20-well precast wide mini gel, TBE, 1.0% plus ethidium bromide, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TBE

161-3030
20-well precast wide mini gel, TBE, 3.0% plus ethidium bromide, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TBE

161-3034
32-well precast wide mini gel, TBE, 1.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TBE

161-3036
32-well precast wide mini gel, TBE, 3.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TBE

161-3038
2 x 32-well comb configuration, precast wide mini gel, TBE, 1.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TBE

161-3040
2 x 32-well comb configuration, precast wide mini gel, TBE, 3.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TAE

161-3044
20-well precast wide mini gel, TAE, 1.0% plus ethidium bromide, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TAE

161-3046
20-well precast wide mini gel, TAE, 3.0% plus ethidium bromide, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TAE

161-3050
32-well precast wide mini gel, TAE, 1.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TAE

161-3052
32-well precast wide mini gel, TAE, 3.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TAE

161-3054
2 x 32-well comb configuration, precast wide mini gel, TAE, 1.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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Wide Mini ReadyAgarose Gel, TAE

161-3056
2 x 32-well comb configuration, precast wide mini gel, TAE, 3.0% plus ethidium bromide, multichannel pipet compatible, 15.6 x 10 cm (tray size)

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ReadyAgarose 96 Plus Gel, TBE

161-3060
4 x 26-well comb configuration (96 plus), precast gel, TBE, 1.0% plus ethidium bromide, 15 x 10 cm, 4- and 12-channel multichannel pipet compatible

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ReadyAgarose 96 Plus Gel, TBE

161-3062
4 x 26-well comb configuration (96 plus), precast gel, TBE, 3.0% plus ethidium bromide, 15 x 10 cm, 4- and 12-channel multichannel pipet compatible

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ReadyAgarose 96 Plus Gel, TAE

161-3063
4 x 26-well comb configuration (96 plus), precast gel, TAE, 1.0% plus ethidium bromide, 15 x 10 cm, 4- and 12-channel multichannel pipet compatible

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ReadyAgarose 96 Plus Gel, TAE

161-3065
4 x 26-well comb configuration (96 plus), precast gel, TAE, 3.0% plus ethidium bromide, 15 x 10 cm, 4- and 12-channel multichannel pipet compatible

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ReadyAgarose Instruction Manual

161-3000
Free on request with purchase of any ReadyAgarose gel

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Number Description Options
5840 Molecular Imager Gel Doc XR+ and ChemiDoc XRS+ Systems Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
2647 ReadyAgarose Precast Gel Flier, Rev C Click to download
2648 ReadyAgarose Precast Gel Brochure, Rev A Click to download
2810 Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A Click to download
6048 Mini-PROTEAN TGX Precast Gels Quick Start Guide, Rev D Click to download [ Add to Cart (Free) ]