DEAE Affi-Gel Blue Gel

DEAE Affi-Gel Blue Gel

Overview

DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron blue F3GA and diethylaminoethyl groups to Bio-Gel A.

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Description

Description

DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron Blue F3GA and diethylaminoethyl groups to Bio-Gel A Gel. The Cibacron blue F3GA functions as an ionic, hydrophobic, or sterically active binding site for proteins with dinucleotide folds, such as albumin. The diethylaminoethyl functional group acts as an anion exchanger and binds proteins with isoelectric points higher than the pH of the mobile phase. This bifunctionality allows you to obtain highly purified IgG from serum from a wide variety of species through careful optimization of the ionic strength and pH of the application buffer.

Features and Benefits

  • Single-step IgG purification from serum; the eluted IgG contains a small amount of transferrin (samples are diluted approximately 5-fold)
  • Albumin removal
  • No detectable proteolytic activity in the eluted IgG fraction
  • Economical alternative to protein A affinity chromatography

More Information

Instruction Manual, DEAE Affi-Gel Blue Support (Bulletin 9191)

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12009262

Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system

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12009263

Pkg of 5, 5 ml, prepacked affinity columns, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system

Documents

Documents

Number Description Options
4006049
Instruction Manual, DEAE Affi-Gel® Blue Support, Rev A
10000105045
Instruction Manual, EconoFit Affi-Gel Blue and DEAE Affi-Gel Blue Columns