Ready Gel® Zymogram Precast Gels

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Overview

Discontinued Ready Gel® Precast Gel Replacements
Discontinued Ready Gel Precast Gels  Replacement Precast Gel
Tris-HCl Gels
5% Ready Gel Tris-HCl Gel, 10 well, 30 µl (161-1210) 7.5% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1023)
5% Ready Gel Tris-HCl Gel, 12 well, 20 µl (161-1214) 7.5% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1025)
5% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1211) 7.5% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1026)
7.5% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1100) 7.5% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1023)
7.5% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1172) 7.5% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1025)
7.5% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1118) 7.5% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1026)
7.5% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1136) 7.5% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1021)
10% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1101) 10% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1033)
10% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1173) 10% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1035)
10% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1119) 10% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1036)
10% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1137) 10% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1031)
10% Ready Gel Tris-HCl Gel, 9 well, 30 µl  (161-1191) 10% Mini-PROTEAN® TGX™ Gel, 8+1 well, 30 µl (456-1039)
10% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1390) 10% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1031)
12% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1102) 12% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1043)
12% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1174) 12% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl  (456-1045)
12% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1120) 12% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1046)
12% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1138) 12% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1041)
12% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1391) 12% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1041)
15% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1175) Any kD™ Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-9035)
15% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1121) Any kD™ Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-9036)
15% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1139) Any kD™ Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-9031)
18% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1216) Any kD™ Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-9033)
18% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1220) Any kD™ Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-9035)
18% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1217) Any kD™ Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-9036)
4–15% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1104) 4–15% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1083)
4–15% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1176) 4–15% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1085)
4–15% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1140) 4–15% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1081)
4–15% Ready Gel Tris-HCl Gel, 9 well, 30 µl  (161-1194) 4–15% Mini-PROTEAN® TGX™ Gel, 8+1 well, 30 µl (456-1089)
4–15% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1392) 4–15% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1081)
4–20% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1177) 4–20% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1095)
4–20% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1141) 4–20% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1091)
4–20% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1393) 4–20% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1091)
8–16% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1222) 8–16% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1103)
8–16% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1226) 8–16% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1105)
8–16% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1223) 8–16% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1106)
8–16% Ready Gel Tris-HCl Gel, 10 well, 50 µl  (161-1225) 8–16% Mini-PROTEAN® TGX™ Gel, 10 well, 50 µl (456-1104)
8–16% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1394) 8–16% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1101)
10–20% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1106) 8–16% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1103)
10–20% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1178) 8–16% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1105)
10–20% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1142) 8–16% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1101)
10–20% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1395) 8–16% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1101)
Isoelecric Focusing (IEF) Gels
pH 3–10 Ready Gel IEF Gel, 10 well, 30 µl  (161-1111) pH 3–10 Criterion IEF Gel, 12+2 well, 45 µl (345-0071)
pH 3–10 Ready Gel IEF Gel, 15 well, 15 µl  (161-1129) pH 3–10 Criterion IEF Gel, 18 well, 30 µl (345-0072)
Zymogram Gels
10% Ready Gel Zymogram Gel, 10 well, 30 µl  (161-1113) 10% Criterion™ Zymogram Gel, 12+2 well, 45 µl (345-0079)
10% Ready Gel Zymogram Gel, 12 well, 20 µl  (161-1185) 10% Criterion Zymogram Gel, 12+2 well, 45 µl (345-0079)
10% Ready Gel Zymogram Gel, 15 well, 15 µl  (161-1131) 10% Criterion Zymogram Gel, 18 well, 30 µl (345-0080)
12% Ready Gel Zymogram Gel, 10 well, 30 µl  (161-1114) 12.5% Criterion Zymogram Gel, 12+2 well, 45 µl (345-0082)
TBE-Urea Gels
5% Ready Gel TBE-Urea Gel, 15 well, 15 µl  (161-1133) 5% Criterion TBE-Urea Gel, 18 well, 30 µl (345-0086)
Migration Pattern for Ready Gel Zymogram Gels

The banding patterns indicate the optimal separation ranges (in kD) for each acrylamide percentage in combinations with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Mini-Format Precast Gel Selection Guide
Refer to the following table to select the appropriate gel type and buffers for your polyacrylamide gel-based applications.
 
Gel Type


Applications



Sample Buffer Running Buffer
TGX™
TGX Stain-Free™



SDS-PAGE
Native PAGE




Laemmli, Native Tris/Glycine/SDS
Tris/Glycine

Tris-Tricine


SDS-PAGE



Tricine Tris/Tricine/SDS
IEF


IEF



IEF IEF Anode and Cathode buffers
Zymogram


Protease detection



Zymogram Tris/Glycine/SDS
TBE
TBE-Urea
Nucleic acids Nucleic Acid, TBE-Urea TBE


Discontinued Product
A number of Ready Gel gels have been discontinued and replaced with Mini-PROTEAN® or Criterion™ gels. However, we will continue to provide support for customers using Ready Gel gels.

See the complete list of discontinued Ready Gel precast gels and corresponding replacement gels.

 

Zymogram gels contain either gelatin or casein, which are substrates for various proteases. Samples are run under denaturing but nonreducing conditions, then allowed to renature and consume the substrate. Samples with proteolytic activity can be visualized as clear bands against a blue background after Coomassie Blue R-250 staining.

Ready Gel® precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by electrophoresis on Ready Gel precast gels using the Mini-PROTEAN® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN® 3 Dodeca™ cell (1–12 gels).

Gel dimensions (W x L x thickness) 8.6 x 6.8 x 0.1 cm
Cassette dimensions (W x L x thickness) 10 x 8 x 0.4 cm
Gel storage conditions Store flat at 4°C; do not freeze
Gel shelf life* 10–12 weeks
* From date of manufacture.  
(Discontinued) 10% Ready Gel® Zymogram Gel, 10 well, 30 µl

161-1113
Discontinued item
Replaced with 10% Criterion™ Zymogram Gel, 12+2 well, 45 µl

This product is no longer available. Please contact technical support for more information on replacement product.
10% Ready Gel® Zymogram Gel, 12 well, 20 µl

161-1185
Discontinued item
Replaced with 10% Criterion™ Zymogram Gel, 12+2 well, 45 µl

List Price:   Inquire
Quantity:  
 
10% Ready Gel® Zymogram Gel, 15 well, 15 µl

161-1131
Discontinued item
Replaced with 10% Criterion™ Zymogram Gel, 18 well, 30 µl

List Price:   Inquire
Quantity:  
 
10% Ready Gel® Zymogram Gel, 10 well, 50 µl

161-1167
Pkg of 1, 10% precast polyacrylamide gel with gelatin, 8.6 × 6.8 cm (W × L), for use with Mini-PROTEAN® electrophoresis cells

List Price:   $13.00
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12% Ready Gel® Zymogram Gel, 10 well, 30 µl

161-1114
Discontinued item
Replaced with 12.5% Criterion™ Zymogram Gel, 12+2 well, 45 µl

List Price:   Inquire
Quantity:  
 
12% Ready Gel® Zymogram Gel, 10 well, 50 µl

161-1168
Pkg of 1, 12% precast polyacrylamide gel with casein, 8.6 × 6.8 cm (W × L), for use with Mini-PROTEAN® electrophoresis cells

List Price:   $13.00
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Number Description Options
5535 Mini-PROTEAN Electrophoresis System Brochure, Rev E Click to download [ Add to Cart (Free) ]
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel System Resource Guide (Note: large PDF file - 3.8 MB) Click to download
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download
4106111 Instruction Manual, Zymogram Sample Buffer, Rev B Click to download