Ready Gel Precast Gels for 2-D Electrophoresis

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Ready Gel Precast Gels for Mini-PROTEAN Systems

Overview

Migration Pattern for Ready Gel Tris-HCI Gels

Standard protein migration on Ready Gel Tris-HCI precast gels. The banding patterns indicate the optimal separation ranges (in kD) for each acrylamide percentage in combinations with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

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The precast gels available for the Mini-PROTEAN® Tetra and Mini-PROTEAN® Dodeca™ cells are 8.6 x 6.8 cm Tris-HCl polyacrylamide gels in single-use cassettes. This gel size provides reproducible, rapid results with fast setup, loading, and run times. For 2-D applications, the Ready Gel IPG combs are designed to hold 7 cm ReadyStrip IPG strips.

Ready Gel precast gels are packaged and sold in individual units: 1–4 gels can be run in the Mini-PROTEAN Tetra cell, and 1–12 gels can be run in the high-throughput Mini-PROTEAN Dodeca cell.

Gel Selection

Use the protein migration chart to select a gel percentage that will provide optimal resolution of your sample.

Use single-percentage gels if your sample contains a limited range of molecular weights, and choose the percentage in which the proteins of interest will migrate to the lower half of the gel.

Single-percentage gels provide the most effective resolution of proteins that are similar in molecular weight, and the best separation on single-percentage gels occurs in the lower half of the gel.

Use gradient gels if your sample contains a broad range of molecular weights. Gradient gels provide the most effective separation for samples across the range of molecular weights, and separation is optimized along the entire length of the gel. Molecules with a range of sizes can be separated on linear gradient gels because the larger pore sizes allow resolution of larger molecules, while the decreasing pore sizes toward the bottom of the gel restrict excessive separation of small molecules.

Number Description Options
5535 Mini-PROTEAN® Electrophoresis System Brochure, Rev F Click to download [ Add to Cart (Free) ]
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel® System Resource Guide Click to download
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download
4006105 Instruction Manual, Isoelectric Focusing Gel Sample Buffer (IEF Gel), Rev C Click to download

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