Ready Gel IEF Precast Gels

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Overview

Discontinued Ready Gel® Precast Gel Replacements
Discontinued Ready Gel Precast Gels  Replacement Precast Gel
Tris-HCl Gels
5% Ready Gel Tris-HCl Gel, 10 well, 30 µl (161-1210) 7.5% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1023)
5% Ready Gel Tris-HCl Gel, 12 well, 20 µl (161-1214) 7.5% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1025)
5% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1211) 7.5% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1026)
7.5% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1100) 7.5% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1023)
7.5% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1172) 7.5% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1025)
7.5% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1118) 7.5% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1026)
7.5% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1136) 7.5% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1021)
10% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1101) 10% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1033)
10% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1173) 10% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1035)
10% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1119) 10% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1036)
10% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1137) 10% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1031)
10% Ready Gel Tris-HCl Gel, 9 well, 30 µl  (161-1191) 10% Mini-PROTEAN® TGX™ Gel, 8+1 well, 30 µl (456-1039)
10% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1390) 10% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1031)
12% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1102) 12% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1043)
12% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1174) 12% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl  (456-1045)
12% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1120) 12% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1046)
12% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1138) 12% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1041)
12% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1391) 12% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1041)
15% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1175) Any kD™ Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-9035)
15% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1121) Any kD™ Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-9036)
15% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1139) Any kD™ Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-9031)
18% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1216) Any kD™ Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-9033)
18% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1220) Any kD™ Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-9035)
18% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1217) Any kD™ Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-9036)
4–15% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1104) 4–15% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1083)
4–15% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1176) 4–15% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1085)
4–15% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1140) 4–15% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1081)
4–15% Ready Gel Tris-HCl Gel, 9 well, 30 µl  (161-1194) 4–15% Mini-PROTEAN® TGX™ Gel, 8+1 well, 30 µl (456-1089)
4–15% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1392) 4–15% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1081)
4–20% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1177) 4–20% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1095)
4–20% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1141) 4–20% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1091)
4–20% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1393) 4–20% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1091)
8–16% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1222) 8–16% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1103)
8–16% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1226) 8–16% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1105)
8–16% Ready Gel Tris-HCl Gel, 15 well, 15 µl  (161-1223) 8–16% Mini-PROTEAN® TGX™ Gel, 15 well, 15 µl (456-1106)
8–16% Ready Gel Tris-HCl Gel, 10 well, 50 µl  (161-1225) 8–16% Mini-PROTEAN® TGX™ Gel, 10 well, 50 µl (456-1104)
8–16% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1394) 8–16% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1101)
10–20% Ready Gel Tris-HCl Gel, 10 well, 30 µl  (161-1106) 8–16% Mini-PROTEAN® TGX™ Gel, 10 well, 30 µl (456-1103)
10–20% Ready Gel Tris-HCl Gel, 12 well, 20 µl  (161-1178) 8–16% Mini-PROTEAN® TGX™ Gel, 12 well, 20 µl (456-1105)
10–20% Ready Gel Tris-HCl Gel, Prep+1 well, 450 µl  (161-1142) 8–16% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1101)
10–20% Ready Gel Tris-HCl Gel, 7 cm IPG/prep well, 250 µl  (161-1395) 8–16% Mini-PROTEAN® TGX™ Gel, 7 cm IPG/prep well, 250 µl (456-1101)
Isoelecric Focusing (IEF) Gels
pH 3–10 Ready Gel IEF Gel, 10 well, 30 µl  (161-1111) pH 3–10 Criterion IEF Gel, 12+2 well, 45 µl (345-0071)
pH 3–10 Ready Gel IEF Gel, 15 well, 15 µl  (161-1129) pH 3–10 Criterion IEF Gel, 18 well, 30 µl (345-0072)
Zymogram Gels
10% Ready Gel Zymogram Gel, 10 well, 30 µl  (161-1113) 10% Criterion™ Zymogram Gel, 12+2 well, 45 µl (345-0079)
10% Ready Gel Zymogram Gel, 12 well, 20 µl  (161-1185) 10% Criterion Zymogram Gel, 12+2 well, 45 µl (345-0079)
10% Ready Gel Zymogram Gel, 15 well, 15 µl  (161-1131) 10% Criterion Zymogram Gel, 18 well, 30 µl (345-0080)
12% Ready Gel Zymogram Gel, 10 well, 30 µl  (161-1114) 12.5% Criterion Zymogram Gel, 12+2 well, 45 µl (345-0082)
TBE-Urea Gels
5% Ready Gel TBE-Urea Gel, 15 well, 15 µl  (161-1133) 5% Criterion TBE-Urea Gel, 18 well, 30 µl (345-0086)
Migration Pattern for Ready Gel IEF Gels

The banding patterns indicate the optimal separation by isoelectric point (pI) for each acrylamide percentage in combination with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Mini-Format Precast Gel Selection Guide
Refer to the following table to select the appropriate gel type and buffers for your polyacrylamide gel-based applications.
 
Gel Type


Application



Sample Buffer Running Buffer
Tris/Glycine


SDS-PAGE
Native PAGE




Laemmli
Native
Tris/glycine/SDS
Tris/glycine

Tris-Tricine


SDS-PAGE



Laemmli Tris/Tricine/SDS
IEF


IEF



IEF Anode and cathode buffer
Zymogram


Protease detection



Zymogram Tris/glycine/SDS


Discontinued Product
A number of Ready Gel gels have been discontinued and replaced with Mini-PROTEAN® or Criterion™ gels. However, we will continue to provide support for customers using Ready Gel gels.

See the complete list of discontinued Ready Gel precast gels and corresponding replacement gels.

 

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than on their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.

Ready Gel® precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by electrophoresis on Ready Gel precast gels using the Mini-PROTEAN® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN® 3 Dodeca™ cell (1–12 gels).

Gel dimensions (W x L x thickness) 8.6 x 6.8 x 0.1 cm
Cassette dimensions (W x L x thickness) 10 x 8 x 0.4 cm
Gel storage conditions Store flat at 4°C; do not freeze
Gel shelf life* ~26 weeks
* From date of manufacture.  
pH 3–10 Ready Gel® IEF Gel, 10 well, 30 µl

161-1111
Discontinued item
Replaced with pH 3–10 Criterion™ IEF Gel, 12+2 well, 45 µl

List Price:   $13.00
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This product is not available for sale online. Please contact customer service for more information.
 
pH 3–10 Ready Gel® IEF Gel, 15 well, 15 µl

161-1129
Discontinued item
Replaced with pH 3–10 Criterion™ IEF Gel, 18 well, 30 µl

List Price:   $13.00
Quantity:   Add to Quote
This product is not available for sale online. Please contact customer service for more information.
 
pH 3–10 Ready Gel® IEF Gel, 10 well, 50 µl

161-1165
Pkg of 1, pH 3–10 precast polyacrylamide gel, 8.6 × 6.8 cm (W × L), for use with Mini-PROTEAN® electrophoresis cells

List Price:   $13.00
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pH 5–8 Ready Gel® IEF Gel, 10 well, 30 µl

161-1112
Pkg of 1, pH 5–8 precast polyacrylamide gel, 8.6 × 6.8 cm (W × L), for use with Mini-PROTEAN® electrophoresis cells

List Price:   $13.00
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Number Description Options
5535 Mini-PROTEAN Electrophoresis System Brochure, Rev E Click to download [ Add to Cart (Free) ]
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel System Resource Guide (Note: large PDF file - 3.8 MB) Click to download
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download
4006105 Instruction Manual, Isoelectric Focusing Gel Sample Buffer (IEF Gel), Rev C Click to download