Rat Diabetes Assays

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Overview

Bio-Plex Pro™ Rat Diabetes Assays

Singleplex Sets
Bead Regions
Ghrelin
64
GLP-1
62
Glucagon
63
Leptin
65
PAI-1
61
Specifications for the NEW Bio-Plex Pro™ Rat Diabetes Assays

Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for <20% intra-assay CV, <30% inter-assay CV, and recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.


Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml Assay Precision
Targets
LLOQ
ULOQ
LOD
Intra-assay %CV Inter-assay %CV
Ghrelin
1
 
16,152
 
0.3
  4 4
GLP-1
4
 
6,062
 
3
  8 6
Glucagon
6
 
4,443
 
6
  3 4
Leptin
24
 
130,465
 
5
  4 3
PAI-1
49
 
66,888
 
27
  5 3

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

Bio-Plex Assay Manuals, Guides and Protocols Library

General Guides and Worksheet
Calculation Worksheet
10010747_45-49 Calculation Worksheet for Bio-Plex Pro™ Assays
General Guides
6457 Bio-Plex® Success Guide for Multiplex Immunoassays — Hardware/Software
6458 Bio-Plex® Success Guide for Multiplex Immunoassays — Assay Sample Prep
4110051 Bio-Plex®  Cell Lysis Kit Product Insert
Human Inflammation Assays
10044281 Bio-Plex Pro™ Human Inflammation Assays Product Information Sheet,
10044282 Instruction Manual, Bio-Plex Pro™ Human Inflammation Panel 1
6625 Bio-Plex Pro™ Inflammation Assays Product Information Sheet
Cytokine, Chemokine, and Growth Factor Assays
Human Cytokine, Chemokine, and Growth Factor Assays
10024985 Bio-Plex Pro™ Assays Quick Guide 4
10014905 Instruction Manual, Bio-Plex Pro™ Cytokine, Chemokine, and Growth Factors Assays
Human Chemokine Assays
10031991 Bio-Plex Pro™ Chemokine Assays Quick Guide
10031990 Instruction Manual, Bio-Plex Pro™ Human Chemokine Assays
Human Th17 Cytokine Assays
10022581 Bio-Plex Pro™ Assays Quick Guide 1
10023381 Instruction Manual, Bio-Plex Pro™ Human Th17 Cytokine Assays
Mouse Cytokine, Chemokine, and Growth Factor Assays, including Th17
10024985 Bio-Plex Pro™ Assays Quick Guide 4
Rat Cytokine, Chemokine, and Growth Factor Assays
10024985 Bio-Plex Pro™ Assays Quick Guide 4
10014905 Instruction Manual, Bio-Plex Pro™ Cytokine, Chemokine, and Growth Factors Assays
TGF-β Assays
10024986 Bio-Plex Pro™ Assays Quick Guide 5
10024984 Instruction Manual, Bio-Plex Pro™ TGF-β Assays
Human MMP and TIMP Assays
10041637 Bio-Plex Pro™ Human MMP Assays Quick Guide
10041638 Bio-Plex Pro™ Human TIMP Assays Quick Guide
10041639 Instruction Manual, Bio-Plex Pro™ Human MMP Assays
10041640 Instruction Manual, Bio-Plex Pro™ Human TIMP Assays
Cell Signaling Assays
10024930 Bio-Plex Pro™ Assays, Quick Guide 3
10024929 Instruction Manual, Bio-Plex Pro™ Cell Signaling Assays
Apoptosis Assays
RBM Apoptosis Assays
10033632 Bio-Plex Pro™ RBM Apoptosis Assays, Quick Guide
10033631 Instruction Manual, Bio-Plex Pro™ RBM Apoptosis Assays
Diabetes, Metabolic, and Hormone Assays
Human, Mouse, and Rat Diabetes Assays
10024973 Bio-Plex Pro™ Assays Quick Guide 2
10010747 Instruction Manual, Bio-Plex Pro™ Diabetes Assays
RBM Human, Metabolic, and Hormone Assays
10041817 Bio-Plex Pro™ RBM Metabolic and Hormone Assays Quick Guide
10041818 Instruction Manual, Bio-Plex Pro™ RBM Metabolic and Hormone Assays
10042014 Bio-Plex Pro™ RBM IGF and IGFBP Assays Quick Guide
10042015 Instruction Manual, Bio-Plex Pro™ RBM IGF and IGFBP Assays
Kidney Toxicity Assays
RBM Human, Rat and Canine Kidney Toxicity Assays
10028259 Bio-Plex Pro™ RBM Kidney Toxicity Assays Quick Guide
10028258 Instruction Manual, Bio-Plex Pro™ RBM Kidney Toxicity Assays
Disease Research Assays
Human Cancer Biomarker Assays
10022581 Bio-Plex Pro™ Assays Quick Guide 1
10022186 Instruction Manual, Bio-Plex Pro™ Human Cancer Biomarker Assays
Human Cytokine Assays
10008318 Instruction Manual, Bio-Plex Precision Pro™ Cytokine Assays
Human Acute Phase Assays
10011597 Instruction Manual, Bio-Plex Pro™ Acute Phase Assays
Human Angiogenesis Assays
10010584 Instruction Manual, Bio-Plex Pro™ Angiogenesis Assays
Human Isotyping Assays
10028208 Bio-Plex Pro™ Assays Quick Guide 6
10028370 Instruction Manual, Bio-Plex Pro™ Human Isotyping Assays
Bio-Plex® Technology and Applications


Bio-Plex® Assay Finder

Simply select your targets of interest to find the assay that meets your needs.


Bio-Plex Assay Finder

Bio-Plex Custom Assays

Create a Bio-Plex Custom Assay

Design your Bio-Plex Assay in four
easy steps.

Bio-Plex Custom Assays

Bio-Plex Pro™ rat diabetes assays are magnetic bead–based assays designed to measure five obesity biomarkers, using as little as 12.5 µl of sample per well. These assays are compatible with the new Bio-Plex Pro rat cytokine, chemokine, and growth factor assays for an expanded biomarker profile. They have been developed to deliver accurate, reproducible measurements with complete flexibility to meet your research needs.

Key Features and Benefits

  • Quality — designed for enhanced assay sensitivity and extended assay working ranges
  • Robustness — tested in serum and plasma sample matrices
  • Increased productivity — measure multiple markers simultaneously in approximately 4 hr
  • Option to use a magnetic wash station — take advantage of the magnetic workflow for simplified assay preparation and realize consistently improved reproducibility (also compatible with the vacuum wash station)

Flexible Ordering Options

  • Singleplex sets — when you want to test for a single analyte
  • Customized Express assays — use the Bio-Plex Assay Builder to select your analytes of interest; please allow an extra 12 days for custom mixing and quality control testing

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Incubation time, hr 1
Sample volume, µl 12.5
Accuracy (recovery range) 80–120%
Precision  
      Intra-assay% CV <20%
      Intra-assay% CV <30%
x

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Bio-Plex Pro Rat Metabolic Ghrelin Set
Bio-Plex Pro™ Rat Diabetes Ghrelin Set

171L7001M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat ghrelin, requires reagent kit (#171-304070) and standards, which are not included

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$280.00
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Bio-Plex Pro Rat Diabetes GLP-1 Set
Bio-Plex Pro™ Rat Diabetes GLP-1 Set

171L7003M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat GLP-1, requires reagent kit (#171-304070) and standards, which are not included

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$280.00
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Bio-Plex Pro Rat Diabetes Glucagon Set
Bio-Plex Pro™ Rat Diabetes Glucagon Set

171L7004M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat glucagon, requires reagent kit (#171-304070) and standards, which are not included

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$280.00
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Bio-Plex Pro Rat Metabolic Leptin Set
Bio-Plex Pro™ Rat Diabetes Leptin Set

171L7006M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat leptin, requires reagent kit (#171-304070) and standards, which are not included

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$280.00
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Bio-Plex Pro™ Rat Diabetes PAI-1 Set
Bio-Plex Pro™ Rat Diabetes PAI-1 Set

171L7007M
1 x 96-well, includes conjugated magnetic beads and detection antibodies for detecting rat PAI-1, requires reagent kit (#171-304070) and standards, which are not included

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$280.00
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Number Description Options
5405 Bio-Plex® Multiplex System Brochure, Ver E Click to download [ Add to Cart (Free) ]
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Rev E Click to download [ Add to Cart (Free) ]
6119 Multiplex Analysis Using Bio-Plex Pro Rat Cytokine and Diabetes Assays, Rev A Click to download
10010747 Instruction Manual, Bio-Plex™ Pro Diabetes Assays, Rev D Click to download
6342 Bio-Plex Pro Diabetes Assay Panels Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
10024973 Bio-Plex Pro Assays Quick Guide 2, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download