RAPID'Staph Medium

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Overview

Key Benefits
  • Complete results in 24 hr
  • Selective
  • Easy alternative confirmation

Protocol
Enumeration of Coagulase-positive Staphylococci
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/09-02/05
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID’Staph protocol is certified by AFNOR Certification, according to the ISO 16140 requirements for the detection of Staphylococcus aureus. (No. BRD 07/09-02/05) and by AOAC-RI (No. 080602).



Intended Use

RAPID'Staph Medium is a selective agar used for the direct enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) in products intended for human or animal consumption or in environmental samples.

Key Benefits

  • Results in 24 hr after 24 hr of enrichment
  • Highly selective and easy to read
  • Choice of two methods for easy confirmation
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

The principle of RAPID'Staph Medium is based on an optimized Baird-Parker formula to guarantee the detection and enumeration of S. aureus in 24 hr at 37°C. Typical staphylococci produce gray to black colonies that are shiny and convex (1–2 mm) with a clear 2–5 mm halo due to proteolysis of the egg yolk.

Presumptive colonies can be confirmed using one of the following methods:

  • Pastorex™ Staph-Plus Latex Agglutination Test (56356) with results within minutes
  • Spot inoculation on Baird-Parker + RPF agar (3563996) to confirm many colonies on the same plate

RAPID'Staph Chromogenic Assay

Enzymatic Activity
Staphylococcus
coagulase-positive

Staphylococcus
coagulase-negative
Potassium tellurite reduction + +
Egg yolk proteolysis + -
     

Ordering Information

RAPID'Staph Medium is available as a package of 20 ready-to-use agar plates (3563960) and as a dehydrated broth agar base (3564704).

When using the dehydrated broth base, Egg Yolk with Potassium Tellurite Supplement (3554201), (3554205) and Sulfamethazine Solution Supplement (3562682) need to be added to the molten agar prior to pouring plates.

Assay type Chromogenic media
Organism Staphylococcus aureus, coagulase-positive staphylococci
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RAPID'Staph Agar #356-3960
RAPID'Staph Agar Plates

3563960
Pkg of 20, 90 mm agar plates

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$37.00
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RAPID'Staph Agar Base, dehydrated #356-4704
RAPID'Staph Agar Base, dehydrated

3564704
Pkg of 1, 500 g of dehydrated agar base, enough for 8.7 L of medium

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$150.00
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Egg Yolk with Potassium Tellurite Supplement #355-4201
Egg Yolk with Potassium Tellurite Supplement, 5 ml

3554201
Pkg of 1, 5 ml vial

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$13.00
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Egg Yolk with Potassium Tellurite Supplement #355-4205
Egg Yolk with Potassium Tellurite Supplement, 25 ml

3554205
Pkg of 1, 25 ml bottle

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$14.00
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Sulfamethazine Solution (0.2%) Supplement #356-2682
Sulfamethazine Solution (0.2%) Supplement

3562682
Pkg of 1, 2.5 ml vial

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$23.00
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Baird-Parker + RPF Agar Kit, with RPF supplement

3578618
Pkg of 1, kit contains 6 x 90 ml bottles of base medium and 6 vials of rabbit plasma fibrinogen (RFP) supplement

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$115.00
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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
TS_RapidStaph RAPID'Staph Agar Technical Sheet v. 3 (U.S. and Canada) Click to download
TS_RapidStaph_en RAPID'Staph Agar Technical Sheet v. 3 (all countries except U.S. and Canada) Click to download
FT_Rstaph_fr Gélose RAPID’Staph Fiche Technique (Tous pays, sauf USA and Canada) Click to download
5533 Evaluation of RAPID'Staph Medium for Enumeration of Coagulase-Positive Staphylococcus aureus in Selected Foods, Rev A Click to download