RAPID’Staph medium is a selective agar used for the direct enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) at 37°C in 24 hr in products intended for human or animal consumption or in environmental samples. Presumptive colonies can be confirmed using one of the following:
- Pastorex®Staph-Plus latex test with results within minutes
- Spot inoculate on Baird-Parker + RPF agar to confirm many colonies on the same plate
The principle of RAPID’Staph medium is based on an optimized Baird-Parker formula to guarantee the detection and enumeration of S. aureus in 24 hr. Typical staphylococci produce gray to black colonies that are shiny and convex (1–2 mm) with a clear halo around the colony (2–5 mm) due to proteolysis of the egg yolk.