RAPID'Salmonella Medium

RAPID'Salmonella Medium

High-sensitivity medium for the detection of Salmonella spp. in human and animal food products. 48 hr to results.

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Ordering Information

Overview

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RAPID'Salmonella Medium is a sensitive chromogenic agar used for the detection and enumeration of Salmonella spp. in products that are intended for human and animal consumption.

Key Benefits

  • Fast results

    • Less than 42 hr to detect Salmonella spp. when using the short protocol
    • Fast confirmation (less than 1 minute)
  • 1 broth and 1 plate

    • Saves money
    • Saves handling time
  • Low false positive rate (<0.3% for all matrices)

    • Saves on unnecessary confirmations
  • Meets regulatory expectations

    • Detects motile and non-motile Salmonella, as well as lactose-positive SalmonellaSalmonella Typhi, and Salmonella Paratiphy
    • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

The high specificity and high selectivity of RAPID'Salmonella Medium are due to a combination of enzymatic activities and the inhibition of contaminating food flora.

The principle of RAPID'Salmonella Medium relies on the expression of two enzymatic activities. Salmonella spp. takes the form of easily identifiable typical magenta colonies (due to the activity of a C8 esterase) in 24 hr.

Counter selection (β-D glucosidase) is used in RAPID'Salmonella Medium to reveal other bacteria with a different color. RAPID'Salmonella Medium can detect motile and non-motile Salmonella, as well as lactose-positive Salmonella, including S. Typhi and S. Paratyphi.

RAPID'Salmonella Chromogenic Assay

rapid-salmonella
Enzymatic Activity
Salmonella spp. Salmonella spp.
Other Enterobacteriaceae Other Enterobacteriaceae
Proteus spp. Proteus spp.
C8 esterase yes yes no
β-D-Glucosidase no yes no

Rapid and Cost-Effective Method

RAPID'Salmonella Medium can be used with a shortened protocol to obtain full results within 42 hr using one plate and one broth. The ISO 16140–validated latex confirmation test gives results in 1 hr directly from isolated colonies.

Other Applications of RAPID'Salmonella Chromogenic Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 6579 reference method)
  • To confirm positive tests obtained with the iQ-Check Salmonella II PCR Detection Kit

Ordering Information

RAPID'Salmonella Medium is available in packages of 20 (3563963) or 120 (3563961) ready-to-use plates or as a dehydrated agar base (3564705)

Ordering

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3563961
Pkg of 20, 90 mm agar plates
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3564705
Pkg of 1, 500 g of dehydrated agar broth enough for 11.5 L of medium
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3555773

Pkg of 25, 10 ml culture tubes

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3564324

Pkg of 1, 500 g of dehydrated broth

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3578123
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 reactions
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3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Documents

Number Description Options
10000126748
RAPID'Salmonella, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
3231
Performance summary, RAPID'Salmonella
10000126749
PIF Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
7381
PIF Supplement flyer
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)
TS_RSalmoSup_en
RAPID’Salmonella Supplement Capsule Technical sheet, V1
FT_RSalmoSup_fr
RAPID’Salmonella Capsule Supplément Fiche Technique, V1
TN_Salmonella_Spice
Internal Comparative Evaluation Study of Alternative Enrichment Protocols for Detection of Salmonella in Untreated Spices, Ver A
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7408
Chromogenic Media Reference Guide
7274
Complete Solutions for Service Laboratories