RAPID'Sakazakii Medium

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Overview

  • Improved formulation results in big easy-to-read colored colonies
  • Short protocol eliminates the need for secondary enrichment
  • Results a full day earlier provides a time and cost savings
  • Ability to detect atypical strains
  • Good correlation with ISO method

Intended Use

RAPID’Sakazakii medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula.

Principle

The principle of the medium is based on detection of a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies. The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, can form light purple or gray colonies.

Simple and Rapid Protocol : certified by AFNOR certification according to the ISO 16140

RAPID’Sakazakii medium is formulated for use in standard methods (for example, ISO 22964 reference method). The medium can also be used with a shortened protocol to test “clean” samples such as powdered infant formula with a low level of contamination. The short protocol is used to detect Cronobacter spp. in 24 hr after an 18 hr enrichment in buffered peptone water, eliminating the need for selective enrichment.


Assay type Chromogenic media
Organism Cronobacter, Enterobacter sakazakii
RAPID'sakazakii Agar #356-3971
RAPID'Sakazakii Agar

356-3971
20 x 90 mm

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RAPID'Sakazakii Agar
RAPID'Sakazakii Agar

356-4976
500 g

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Buffered Peptone Water/Broth

355-4179
225 ml x 6 bottles

List Price:   $25.00
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Buffered Peptone Water/Broth

356-4684
500 g

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Buffered Peptone Water/Broth

355-5790
5 L x 2 bags

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Number Description Options
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Click to download