RAPID'P.aeruginosa Agar is a selective chromogenic culture medium for the direct enumeration (without confirmation) of Pseudomonas aeruginosa using the membrane filtration method.
It is particularly suitable for the testing of water for human consumption, in particular bottled water and untreated water such as water from wells, lakes, and streams wherein the commensal water flora is abundant and for which the standardized medium is nonspecific for P. aeruginosa.
The principle of RAPID'P.aeruginosa medium is based on the detection of an enzymatic activity typical of P. aeruginosa. Under its action, a specific chromogenic substrate is cleaved, leading to the formation of a blue to blue-green precipitate on the Pseudomonas aeruginosa colonies.
The selective mixture makes it possible to inhibit the majority of interfering flora, particularly Pseudomonas non-aeruginosa, which is very important in water. Other microorganisms may show growth; however, their colonies appear transparent or pigmented yellow-green and are easily distinguishable from those of Pseudomonas aeruginosa.