RAPID'L.mono Medium

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Overview

Key Benefits
  • Fast
    • 24 hr incubation only for L. monocytogenes detection and enumeration
    • Easy-to-read chromogenic reaction
  • Simple and Affordable
    • Economical (1 broth, 1 plate)
    • Easy confirmation
  • Easy to Read
    • Blue colonies are L. monocytogenes
    • Blue-green colonies with distinct yellow halos are L. ivanovii
    • White colonies are other Listeria species
Protocol

Validation

Validated and Adapted for Food Detection

The RAPID’L.mono method is validated:

  • AFNOR Certification according to the ISO 16140 requirements for the detection of Listeria monocytogenes
    and other Listeria (No. BRD: 07/04-09/98) and for the enumeration of Listeria monocytogenes (No. BRD: 07/05-09/01)
  • AOAC-RI for the detection of Listeria monocytogenes in 24 hr (No030406)
  • NordVal Validation Ref No022
  • FDA/BAM recommended

Intended Use

RAPID’L.mono™ medium is a selective chromogenic medium for direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples.

A Multipurpose Agar

RAPID’L.mono medium is the only chromogenic method to be validated for L. monocytogenes and Listeria spp. detection on the same plate. RAPID’L. mono agar can also be used for enumeration or confirmation of L. monocytogenes.

  • L. monocytogenes detection 24 hr after enrichment*
  • Listeria spp. detection 24 and 48 hr after enrichment
  • L. monocytogenes enumeration in 24 hr without confirmation**
  • L. monocytogenes confirmation in 24 hr after using the iQ-Check® Listeria monocytogenes II kit

Rapid and Economical Method

With the RAPID’L.mono detection protocol, full results can be obtained within 48 hr using one plate and one broth.

Reading and differentiation of L. monocytogenes are easy due to the unique chromogenic principle (PIPLC activity) of RAPID’L.mono medium and the fermentation of a sugar (xylose).

The blue colonies are L. monocytogenes (PIPLC+/xylose–).

The blue colonies with a yellow halo are L. ivanovii (PIPLC+/xylose+).

The white colonies with or without a yellow halo are other Listeria spp. (PIPLC–/xylose+ or –).

RAPID’L.mono medium is very specific; other bacteria and yeasts are inhibited.

Easy and economical confirmation is also validated in the RAPID’L.mono protocol by spot inoculation on Agar Listeria (AL agar) or by the Rhamnose test in 6 hr.

Other Protocols

RAPID’L.mono chromogenic plating agar can be used

  • As a second selective medium in different standardized protocols (for example, ISO 11290 reference method)
  • To confirm positive iQ-Check Listeria monocytogenes II tests
  • To enumerate L. monocytogenes in 24 hr without confirmation

* Within the scope of ISO 16140 NordVal validation, no confirmation is required.

** Within the scope of the AFNOR validation, confirmation is not required when the presence of L. monocytogenes has been confirmed at the detection stage.

Assay type Chromogenic media
Organism Listeria
RAPID'L.mono Agar #355-5294
RAPID'L.mono Agar

355-5294
190 ml + supplement

List Price:   $38.00
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RAPID'L.mono Agar #356-3694
RAPID'L.mono Agar

356-3694
90 mm x 20 dishes

List Price:   $63.00
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RAPID´L.mono (Dehydrated Base)

356-4293
500 g

List Price:   $563.00
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RAPID´L.mono Supplement 1

356-4294
10 vials (1 vial q.s.p. 500 ml)

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RAPID'Listeria Supplement 2 #356-4746
RAPID'Listeria Supplement 2

356-4746
10 vials (1 vial q.s.p. 500 ml)

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Fraser 1/2 Broth #355-5797
Fraser 1/2 Broth

355-5797
225 ml x 6 bottles

List Price:   $41.00
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Fraser Broth Base #356-4604
Fraser Broth Base

356-4604
500 g

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Fraser 1/2 Supplement #356-4616
Fraser 1/2 Supplement

356-4616
10 vials (1 vial q.s.p. 2.25 l)

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Buffered Peptone Water/Broth

355-4179
225 ml x 6 bottles

List Price:   $25.00
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Buffered Peptone Water/Broth

355-5790
5 L x 2 bags

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Buffered Peptone Water/Broth

356-4684
500 g

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Number Description Options
3046 Rapid'L Mono Chromogenic Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Click to download
TS_RLMono TS_RLMono Click to download