RAPID'E.coli O157:H7 Medium

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Overview


Key Benefits

KEY BENEFITS

  • Specific to E.coli O157 serotype H7
  • Only 1 plate required per sample
  • Complete results in 48 hr

Protocol
Detection of Escherichia coli O157:H7
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/14-01/08
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID’E.coli O157:H7 method is certified by AFNOR Certification according to the ISO 16140 requirements for the detection of E. coli O157:H7 (No. BRD 07/14–01/08) and AOAC-RI validated (No. 060701).



Intended Use

RAPID'E.coli O157:H7 Medium is a selective chromogenic agar for the detection, isolation, and presumptive identification of Escherichia coli O157:H7 in human food products and environmental samples.

Key Benefits

  • High specificity to E. coli O157 serotype H7
  • Only 1 plate required per sample
  • Results in 48 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Simple Protocol

RAPID'E.coli O157:H7 Medium provides laboratories with a rapid and cost-effective solution for E. coli O157:H7 detection, given that a single plate is required for each sample. The latex test for confirmation has been validated for use on isolated colonies grown on RAPID'E.coli plates.

Other Applications of RAPID'E.coli O157:H7 Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 16654 reference method)
  • To confirm positive results obtained with the iQ-Check® E. coli O157:H7 PCR Detection Kit

Easy-to-Read Results

RAPID'E.coli O157:H7 medium permits the detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities.

  • Typical E. coli O157:H7 (Sorbitol/SORB +, β-Glucuronidase/GLUC +) colonies are characteristically convex, 1–2 mm in diameter, and dark blue to black in color
  • Atypical GLUC + E. coli O157:H7 are detected and present as dark blue to black colonies
  • Atypical SORB + E. coli O157:H7 are detected and present as blue to turquoise blue colonies

RAPID'E.coli O157:H7 Medium is very specific. Most other bacteria and yeasts are inhibited or produce a different color. The selectivity of RAPID'E.coli O157:H7 Medium can be improved by adding selective agents such as novobiocin and potassium tellurite.

RAPID'E.coli O157:H7 Chromogenic Assay

Enzymatic Activity
E. coli O157:H7
Other E. coli Other bacteria
Glucuronidase - + -
Galactosidase + + -
Sorbitol - + +/-
     

Ordering Information

RAPID'E.coli O157:H7 Medium is available as a dehydrated agar broth (3564748).

Assay type Chromogenic media
Organism E. coli O157:H7
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RAPID'E. coli O157:H7 #356-4748
RAPID'E. coli O157:H7, dehydrated

3564748
Pkg of 1, 100 g of dehydrated agar broth, enough for 1.25 L of medium

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$270.00
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STEC Enrichment Broth
STEC Enrichment Broth, dehydrated

3564001
Pkg of 1, 500 g of dehydrated broth

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$93.00
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mTSB + Novobiocin Broth ready to use #355-5426
Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready to use

3555426
Pkg of 6, 225 ml bottles, contains novobiocin

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$60.00
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mTSB Broth dehydrated (base) #356-4426
Modified Tryptic Soy Broth (mTSB) Base, dehydrated

3564426
Pkg of 1, 500 g of dehydrated base

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$99.00
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Novobiocin #356-4610
Novobiocin, powder

3564610
Pkg of 1, 1 g

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$61.00
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iQ-Check E.coli O157:H7 #357-8114
iQ-Check® E.coli O157:H7 PCR Detection Kit

3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
TS_REC_O157:H7_en RAPID'E.coli O157:H7 Agar Technical Sheet, v. 2 (All countries except U.S. and Canada) Click to download
FT_REC O157 H7_fr RAPID’ E. coli O157:H7/Gélose Fiche Technique (Tous pays, sauf USA & Canada) Click to download
TS_REC_O157:H7 RAPID'E.coli O157:H7 Agar Technical Sheet, v. 2 (U.S. and Canada Only) Click to download