RAPID'E.coli O157:H7 Medium

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RAPID'<em>E.coli</em> O157:H7 Medium

Overview


Key Benefits

KEY BENEFITS

  • Specific to E.coli O157 serotype H7
  • Only 1 plate required per sample
  • Complete results in 48 hr

Protocol
Detection of Escherichia coli O157:H7
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/14-01/08
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID’E.coli O157:H7 method is certified by AFNOR Certification according to the ISO 16140 requirements for the detection of E. coli O157:H7 (No. BRD 07/14–01/08) and AOAC-RI validated (No. 060701).

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Intended Use

RAPID'E.coli O157:H7 Medium is a selective chromogenic agar for the detection, isolation, and presumptive identification of Escherichia coli O157:H7 in human food products and environmental samples.

Key Benefits

  • High specificity to E. coli O157 serotype H7
  • Only 1 plate required per sample
  • Results in 48 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Simple Protocol

RAPID'E.coli O157:H7 Medium provides laboratories with a rapid and cost-effective solution for E. coli O157:H7 detection, given that a single plate is required for each sample. The latex test for confirmation has been validated for use on isolated colonies grown on RAPID'E.coli plates.

Other Applications of RAPID'E.coli O157:H7 Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 16654 reference method)
  • To confirm positive results obtained with the iQ-Check® E. coli O157:H7 PCR Detection Kit

Easy-to-Read Results

RAPID'E.coli O157:H7 medium permits the detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities.

  • Typical E. coli O157:H7 (Sorbitol/SORB +, β-Glucuronidase/GLUC +) colonies are characteristically convex, 1–2 mm in diameter, and dark blue to black in color
  • Atypical GLUC + E. coli O157:H7 are detected and present as dark blue to black colonies
  • Atypical SORB + E. coli O157:H7 are detected and present as blue to turquoise blue colonies

RAPID'E.coli O157:H7 Medium is very specific. Most other bacteria and yeasts are inhibited or produce a different color. The selectivity of RAPID'E.coli O157:H7 Medium can be improved by adding selective agents such as novobiocin and potassium tellurite.

RAPID'E.coli O157:H7 Chromogenic Assay

Enzymatic Activity
E. coli O157:H7
Other E. coli Other bacteria
Glucuronidase - + -
Galactosidase + + -
Sorbitol - + +/-
     

Ordering Information

RAPID'E.coli O157:H7 Medium is available as a dehydrated agar broth (3564748).

Assay type Chromogenic media
Organism E. coli O157:H7
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RAPID'E. coli O157:H7, dehydrated

3564748
Pkg of 1, 100 g of dehydrated agar broth, enough for 1.25 L of medium

0 3564748 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4748_0921121047608.xml 3564748 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA FSD 2017-11-14 07:07:03 3564748-rapide-coli-o157-h7-dehydrated 3564748 RAPID'<em>E. coli</em> O157:H7 RAPID'<em>E. coli</em> O157:H7, dehydrated Pkg of 1, 100 g of dehydrated agar broth, enough for 1.25 L of medium dehydrated, 100 g Pkg of 1, 100 g of dehydrated agar broth, enough for 1.25 L of medium /webroot/web/images/fsd/products/food_testing/sku_thumb/global/356-4748_160.jpg /webroot/web/images/fsd/products/food_testing/sku_view/global/356-4748_view.jpg RAPID'E. coli O157:H7 #356-4748 TW RAPID'E. coli O157:H7 #356-4748 dehydrated, 100 g ecoli o157h7, chromogenic media, color change, detection, enumeration, confirmation, rapid method, food safety 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Food-Testing/Chromogenic-Media N 0 <p>Use RAPID'<em>E.coli</em> O157:H7 Agar for the detection, isolation, and presumptive identification of <em>E. coli</em> O157:H7 in food products. Results should be confirmed by standard reference methods appropriate for the food type being tested. Confirmation using latex is also validated for colonies isolated from RAPID'<em>E.coli</em> O157:H7 agar.</p> <p>This product includes 100 g of dehydrated medium. Supplements of novobiocin (10 mg/L) and potassium tellurite (0.8 mg/L) must be added to this medium.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Detection on a single plate</li> <li>Typical (sorbitol-/&beta;-glucuronidase-) and atypical (&beta;-glucuronidase+) <em>E. coli</em> O157:H7 colonies are dark blue or black</li> <li>Other atypical (sorbitol+) <em>E. coli</em> O157:H7 colonies are blue to turquoise blue</li> <li>100% specificity demonstrated by testing 50 strains of <em>E. coli</em> O157:H7 and 35 other strains</li> <li>Selectivity is increased by the addition of novobiocin and other selective agents such as potassium tellurite</li> <li>Results with <a href="/en-us/product/rapide-coli-o157-h7-medium">RAPID'<em>E.coli</em> O157:H2 Medium</a> in &le;48 hr</li> </ul> <h2 class="descHeader">Validations</h2> <p>Certified NF VALIDATION according to the ISO 16140 standard and validated by the AOAC.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>STEC Enrichment Broth, dehydrated, 500 g (<a href="/en-us/sku/3564001-stec-enrichment-broth">3564001</a>) and 5 kg (<a href="/en-us/sku/3564002-stec-enrichment-broth">3564002</a>)</li> <li>Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready-to-use (<a href="/en-us/sku/3555426-mtsb-novobiocin-broth-ready-use">3555426</a>)</li> <li>Modified Tryptic Soy Broth (mTSB) Base, dehydrated (<a href="/en-us/sku/3564426-mtsb-broth-dehydrated-base">3564426</a>)</li> <li>Novobiocin, powder (<a href="/en-us/sku/3564610-novobiocin">3564610</a>)</li> <li>iQ-Check<sup>&reg;</sup> <em>E. coli</em> O157:H7 Real-Time PCR Kit (<a href="/en-us/sku/3578114-iq-check-e-coli-o157-h7-kit">3578114</a>)</li> <li>iQ-Check STEC VirX Real-Time PCR Kit (<a href="/en-us/sku/3578139-iq-check-stec-virx-kit">3578139</a>) for detection of <em>stx1-stx2</em> and <em>eae</em> virulence genes</li> <li>iQ-Check STEC SerO Real-Time PCR Kit (<a href="/en-us/sku/3578140-iq-check-stec-sero-kit">3578140</a>) for detection of six O serotypes (O26, O111, O45, O145, O121, and O103) plus <em>E. coli </em>O157:H7</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/chromogenic-media">Chromogenic Media</a></li> <li><a href="/en-us/category/food-pcr-testing-kits">Food PCR Testing Kits</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> /webroot/web/html/fsd/products/SKUs/autopopulated/ 356-4748_0921121047608_en.html /webroot/web/html/fsd/products/SKUs/autopopulated//356-4748_0921121047608_en.html Assay type 1351 Chromogenic media 0 0 Organism 2367 E. coli O157:H7 0 0 3564001 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4001_1357810521.xml 3564426 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4426_0921121047629.xml 3564610 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4610_0921121047568.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564748&CartQuote=QT&quantity= false true false $295.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564748&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564748&quantity= 3564748
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STEC Enrichment Broth, dehydrated

3564001
Pkg of 1, 500 g of dehydrated broth

3564001 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4001_1357810521.xml 3564001 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po1hejb6po1hf" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po1hejb6po1hf"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="15" DayOfWeek="5" Year="2017" Hour="1" Minute="48" Second="57" ZoneOffsetMillis="0" Time="1513302537428">Fri Dec 15 01:48:57 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4001_1357810521.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="15" DayOfWeek="5" Year="2017" Hour="1" Minute="48" Second="57" ZoneOffsetMillis="0" Time="1513302537428">Fri Dec 15 01:48:57 UTC 2017</LastExecutionTime> </Node> </Deployments> 01/10/13 01:10 AM en_US jb42cvfw 2017-11-14 07:07:02 true true internet/product_SKU FSD N en 01/09/23 10:34 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:02 3564001-stec-enrichment-broth-dehydrated 3564001 STEC Enrichment Broth STEC Enrichment Broth, dehydrated Pkg of 1, 500 g of dehydrated broth Pkg of 1, 500 g of dehydrated broth STEC Enrichment Broth Martine Mamdy STEC Enrichment Broth 500 g 3564001, stec, enrichment, broth, chromogenic, medium, media, enumeration, differentiation, e. coli, ecoli, e coli, rapid, food, science, safety, testing, environmental 01/10/13 01:10 AM 01/09/23 10:34 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Food-Testing/Chromogenic-Media N 0 <p>Use STEC (Shiga Toxin-Producing <em>E. coli</em>) Enrichment Broth for culturing samples prior to screening and detecting shigatoxigenic <em>E. coli</em> including <em>E. coli</em> O157:H2 in products intended for human and animal consumption.</p> <h2 class="descHeader">Packaging Options</h2> <p>STEC Enrichment Broth is also available as a 5 kg package (<a href="/en-us/sku/3564002-stec-enrichment-broth">3564002</a>)</p> <h2 class="descHeader">Related Products</h2> <ul> <li>RAPID'<em>E. coli </em>O157:H7 Agar, dehydrated (<a href="/en-us/sku/3564748-rapide-coli-o157-h7">3564748</a>)</li> <li>iQ-Check<sup>&reg;</sup> <em>E. coli</em> O157:H7 Real-Time PCR Kit (<a href="/en-us/sku/3578114-iq-check-e-coli-o157-h7-kit">3578114</a>)</li> <li>iQ-Check STEC VirX Real-Time PCR Kit (<a href="/en-us/sku/3578139-iq-check-stec-virx-kit">3578139</a>) for detecting <em>stx1-stx2</em> and <em>eae</em> virulence genes</li> <li>iQ-Check STEC SerO Real-Time PCR Kit (<a href="/en-us/sku/3578140-iq-check-stec-sero-kit">3578140</a><a href="/en-us/sku/3578139-iq-check-stec-virx-kit"></a>) for detecting six O serotypes (O26, O111, O45, O145, O121, and O103) plus <em>E. coli </em>O157:H7</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/chromogenic-media">Chromogenic Media</a></li> <li><a href="/en-fr/category/food-pcr-testing-kits">Food PCR Testing Kits</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> /webroot/web/html/fsd/products/SKUs/autopopulated stec-enrichment-broth-dehydrated-3564001.html /webroot/web/html/fsd/products/SKUs/autopopulated/ stec-enrichment-broth-dehydrated-3564001.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564001&CartQuote=QT&quantity= false true false $96.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564001&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564001&quantity= 3564001
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Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready to use

3555426
Pkg of 6, 225 ml bottles, contains novobiocin

1 3555426 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/355-5426_0921121047588.xml 3555426 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA FSD 2017-11-14 07:07:02 3555426-modified-tryptic-soy-broth-mtsb-novobiocin-ready-use 3555426 mTSB + Novobiocin Broth ready to use Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready to use Pkg of 6, 225 ml bottles, contains novobiocin (containing novobiocin), 225 ml x 6 bottles Pkg of 6, 225 ml bottles, contains novobiocin /webroot/web/images/fsd/products/food_testing/sku_thumb/global/355-5426_160.jpg /webroot/web/images/fsd/products/food_testing/sku_view/global/355-5426_view.jpg /webroot/web/images/fsd/products/food_testing/sku_feature/global/355-5426_250.jpg mTSB + Novobiocin Broth ready to use #355-5426 RepEx mTSB + Novobiocin Broth ready to use #355-5426 (containing novobiocin), 225 ml x 6 bottles enrichment broth, primary media, ecoli o157h7 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Food-Testing/Chromogenic-Media N 0 <p>Use Modified Tryptic Soy Broth (mTSB) + Novobiocin for selective enrichment of<em> E. coli</em> O157:H7 in products intended for human and animal consumption. This broth is designed to be a primary enrichment step for the identification and enumeration using RAPID'<em>E. coli</em> O157:H7 Agar.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Modified Tryptic Soy Broth (mTSB) Base, dehydrated (<a href="/en-us/sku/3564426-mtsb-broth-dehydrated-base">3564426</a>)</li> <li>Novobiocin, powder (<a href="/en-us/sku/3564610-novobiocin">3564610</a>)</li> <li>RAPID'<em>E. coli </em>O157:H7 Agar, dehydrated (<a href="/en-us/sku/3564748-rapide-coli-o157-h7">3564748</a>)</li> <li>iQ-Check<sup>&reg;</sup> <em>E. coli</em> O157:H7 Real-Time PCR Kit (<a href="/en-us/sku/3578114-iq-check-e-coli-o157-h7-kit">3578114</a>)</li> <li>iQ-Check STEC VirX Real-Time PCR Kit (<a href="/en-us/sku/3578139-iq-check-stec-virx-kit">3578139</a>) for detecting <em>stx1-stx2</em> and <em>eae</em> virulence genes</li> <li>iQ-Check STEC SerO Real-Time PCR Kit (<a href="/en-us/sku/3578140-iq-check-stec-sero-kit">3578140</a>) for detecting six O serotypes (O26, O111, O45, O145, O121, and O103) plus <em>E. coli </em>O157:H7</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/chromogenic-media">Chromogenic Media</a></li> <li><a href="/en-fr/category/food-pcr-testing-kits">Food PCR Testing Kits</a></li> <li><a href="/en-fr/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> /webroot/web/html/fsd/products/SKUs/autopopulated/ 355-5426_0921121047588_en.html /webroot/web/html/fsd/products/SKUs/autopopulated//355-5426_0921121047588_en.html Assay type 1351 Enrichment broth 0 0 Organism 2367 E. coli O157:H7 0 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3555426&CartQuote=QT&quantity= false true false $60.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3555426&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3555426&quantity= 3555426
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Modified Tryptic Soy Broth (mTSB) Base, dehydrated

3564426
Pkg of 1, 500 g of dehydrated base

2 3564426 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4426_0921121047629.xml 3564426 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jb6po1hejb6po1hf" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jb6po1hejb6po1hf"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="15" DayOfWeek="5" Year="2017" Hour="1" Minute="48" Second="57" ZoneOffsetMillis="0" Time="1513302537428">Fri Dec 15 01:48:57 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4426_0921121047629.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="15" DayOfWeek="5" Year="2017" Hour="1" Minute="48" Second="57" ZoneOffsetMillis="0" Time="1513302537428">Fri Dec 15 01:48:57 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM en_US jb42cvft 2017-11-14 07:07:02 true true internet/product_SKU FSD N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:02 3564426-modified-tryptic-soy-broth-mtsb-base-dehydrated 3564426 mTSB Broth dehydrated (base) Modified Tryptic Soy Broth (mTSB) Base, dehydrated Pkg of 1, 500 g of dehydrated base 500 g Pkg of 1, 500 g of dehydrated base /webroot/web/images/fsd/products/food_testing/sku_thumb/global/356-4426_160.jpg /webroot/web/images/fsd/products/food_testing/sku_view/global/356-4426.jpg /webroot/web/images/fsd/products/food_testing/sku_feature/global/356-4426_250.jpg mTSB Broth dehydrated (base) #356-4426 RepEx mTSB Broth dehydrated (base) #356-4426 500 g enrichment broth, primary media, ecoli o157h7 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Food-Testing/Chromogenic-Media N 0 <p>Use Modified Tryptic Soy Broth (mTSB) Base, after the addition of novobiocin, for selective enrichment of <em>E. coli</em> O157:H7 in products intended for human and animal consumption. This broth is designed to be a primary enrichment step prior to the identification and enumeration with RAPID'<em>E. coli</em> O157:H7 Agar.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Novobiocin, powder (<a href="/en-us/sku/3564610-novobiocin">3564610</a>)</li> <li>Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready-to-use (<a href="/en-us/sku/3555426-mtsb-novobiocin-broth-ready-use">3555426</a>)</li> <li>RAPID'<em>E. coli </em>O157:H7 Agar, dehydrated (<a href="/en-us/sku/3564748-rapide-coli-o157-h7">3564748</a>)</li> <li>iQ-Check<sup>&reg;</sup> <em>E. coli</em> O157:H7 Real-Time PCR Kit (<a href="/en-us/sku/3578114-iq-check-e-coli-o157-h7-pcr-detection-kit">3578114</a>)</li> <li>iQ-Check STEC VirX Real-Time PCR Kit (<a href="/en-fr/sku/3578139-iq-check-stec-virx-pcr-detection-kit">3578139</a>) for detecting <em>stx1-stx2</em> and <em>eae</em> virulence genes</li> <li>iQ-Check STEC SerO Real-Time PCR Kit (<a href="/en-us/sku/3578140-iq-check-stec-sero-kit">3578140</a>) for detecting six O serotypes (O26, O111, O45, O145, O121, and O103) plus <em>E. coli </em>O157:H7</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/chromogenic-media">Chromogenic Media</a></li> <li><a href="/en-fr/category/food-pcr-testing-kits">Food PCR Testing Kits</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> /webroot/web/html/fsd/products/SKUs/autopopulated/ 356-4426_0921121047629_en.html /webroot/web/html/fsd/products/SKUs/autopopulated//356-4426_0921121047629_en.html Assay type 1351 Enrichment broth 0 0 Organism 2367 E. coli O157:H7 0 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564426&CartQuote=QT&quantity= false true false $99.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564426&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564426&quantity= 3564426
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Novobiocin, powder

3564610
Pkg of 1, 1 g

3 3564610 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4610_0921121047568.xml 3564610 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA FSD 2017-11-14 07:07:03 3564610-novobiocin-powder 3564610 Novobiocin Novobiocin, powder Pkg of 1, 1 g 1 g Pkg of 1, 1 g Novobiocin #356-4610 RepEx Novobiocin #356-4610 1 g antibiotic supplement, e coli o157h7, novobiocin 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Food-Testing/Chromogenic-Media N 0 <p>Use the antibiotic Novobiocin with Modified Tryptic Soy Broth (mTSB) Base for selective enrichment of <em>E. coli</em> O157:H7 in products intended for human and animal consumption. This broth is designed to be a primary enrichment step for the identification and enumeration using RAPID'<em>E. coli</em> O157:H7 Agar.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Modified Tryptic Soy Broth (mTSB) Base, dehydrated (<a href="/en-us/sku/3564426-mtsb-broth-dehydrated-base">3564426</a>)</li> <li>Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready-to-use (<a href="/en-us/sku/3555426-mtsb-novobiocin-broth-ready-use">3555426</a>)</li> <li>RAPID'<em>E. coli </em>O157:H7 Agar, dehydrated (<a href="/en-us/sku/3564748-rapide-coli-o157-h7">3564748</a>)</li> <li>iQ-Check<sup>&reg;</sup> <em>E. coli</em> O157:H7 Real-Time PCR Kit (<a href="/en-us/sku/3578114-iq-check-e-coli-o157-h7-kit">3578114</a>)</li> <li>iQ-Check STEC VirX Real-Time PCR Kit (<a href="/en-us/sku/3578139-iq-check-stec-virx-kit">3578139</a>) for detecting <em>stx1-stx2</em> and <em>eae</em> virulence genes</li> <li>iQ-Check STEC SerO Real-Time PCR Kit (<a href="/en-us/sku/3578140-iq-check-stec-sero-kit">3578140</a>) for detecting six O serotypes (O26, O111, O45, O145, O121, and O103) plus <em>E. coli </em>O157:H7</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-fr/category/chromogenic-media">Chromogenic Media</a></li> <li><a href="/en-fr/category/food-pcr-testing-kits">Food PCR Testing Kits</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> /webroot/web/html/fsd/products/SKUs/autopopulated/ 356-4610_0921121047568_en.html /webroot/web/html/fsd/products/SKUs/autopopulated//356-4610_0921121047568_en.html Assay type 1351 Supplement 0 0 Organism 2367 E. coli O157:H7 0 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564610&CartQuote=QT&quantity= false true false $79.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564610&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3564610&quantity= 3564610
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iQ-Check® E.coli O157:H7 PCR Detection Kit

3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

3578114 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Real-Time-PCR-Kits/357-8114_0921121048702.xml 3578114 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA FSD 2017-11-14 07:07:05 3578114-iq-check-e-coli-o157-h7-pcr-detection-kit 3578114 iQ-Check <em>E.coli</em> O157:H7 iQ-Check<sup>&reg;</sup> <em>E.coli</em> O157:H7 PCR Detection Kit Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples 96 tests Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples /webroot/web/images/fsd/products/food_testing/sku_thumb/global/tax213_eco157_357-8114_160.jpg /webroot/web/images/fsd/products/food_testing/sku_view/global/tax213_eco157_357-8114_275.jpg /webroot/web/images/fsd/products/food_testing/sku_feature/global/tax213_eco157_357-8114_250.jpg iQ-Check E.coli O157:H7 #357-8114 RepEx iQ-Check E.coli O157:H7 #357-8114 Food Safety: The iQ-Check™ system takes full advantage of real-time PCR to provide a faster and reliable solution in FOOD pathogen testing. 3578114, iq-check, e.coli, o157:h7, food, safety, testing, pathogen, detection, pcr, amplification, real-time, real, time, qpcr 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en FSD /FSD/Food-Testing/Real-Time-PCR-Kits N 0 <p>Use the iQ-Check <em>E</em><em>. </em><em>coli</em> O157:H7 PCR Detection Kit for identifying <em>E</em><em>.</em><em>coli</em> O157:H7 DNA sequences in foodstuffs and environmental samples. This test can be run in parallel with other iQ-Check Kits.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Kit contains 96 tests, sufficient for processing 94 samples with positive and negative controls in a single run; store at 2&ndash;8&deg;C</li> <li>Results in as little as 12 hr</li> <li>For raw ground beef, combine with parallel detection of <em>Salmonella</em> with the <a href="/en-us/product/iq-check-salmonella-ii-kit">iQ-Check <em>Salmonella</em> II PCR Detection Kit</a></li> <li>Use for confirmation of single isolated colonies from <em>E. coli</em> O157:H7 agar plates such as <a href="/en-us/product/rapide-coli-o157-h7-medium">RAPID'<em>E.coli </em>O157:H7 Agar</a></li> <li>Use with <a href="/en-us/product/iq-check-stec-kits">iQ-Check STEC VirX and SerO PCR Detection Kits</a> for screening for virulence genes</li> <li>For high throughput, use the <a href="/en-us/product/iq-check-prep-solution">iQ-Check Prep System</a>, a robotic liquid handling platform performing DNA extraction and PCR plate setup</li> </ul> <h2 class="descHeader">Validations</h2> <ul> <li>Certified NF VALIDATION according to the ISO 16140 standard and validated by the AOAC</li> <li>Validated on a wide range of food products including raw beef, produce, and apple cider</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>RAPID'<em>E.coli </em>O157:H7 Agar, dehydrated (<a href="/en-us/sku/3564748-rapide-coli-o157-h7">3564748</a>)</li> <li>Buffered Peptone Water Broth, 6 x 225 ml bottles (<a href="/en-us/sku/3554179-buffered-peptone-water-broth">3554179</a>) and dehydrated, 500 g (<a href="/en-us/sku/3564684-buffered-peptone-water-broth">3564684</a>)</li> <li>STEC Enrichment Broth, dehydrated, 500 g (<a href="/en-us/sku/3564001-stec-enrichment-broth">3564001</a>) and 5 kg (<a href="/en-us/sku/3564002-stec-enrichment-broth">3564002</a>)</li> <li>Heating Thermoshaker (<a href="/en-us/sku/3594999-heating-thermoshaker">3594999</a>) and iQ-Check Deep Well Microplates (<a href="/en-us/sku/3594900-iq-check-deep-well-microplates">3594900</a>) for high-temperature cell lysis and DNA extraction</li> <li>Digital Disruptor Genie Cell Disruptor (<a href="/en-us/sku/3591456-digital-disruptor-genie-cell-disruptor">3591456</a>) mechanical disruptor for use with microtubes</li> <li class="hidden US-only CA-only AT-only BE-only DK-only FI-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only">1.5 ml Conical Tubes, screwcap with O-rings, sterile (<a href="/en-us/sku/2240110xtu-1-5-ml-conical-tubes-screwcap-sterile">2240110XTU</a>)</li> <li class="hidden US-only CA-only AT-only BE-only DK-only FI-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only">Multiplate&trade; 96-Well (<a href="/en-us/sku/mll9651xtu-multiplate-96-well-pcr-plates-low-profile-unskirted">MLL9651XTU</a>) and 48-Well (<a href="/en-us/sku/mll4851xtu-multiplate-48-well-pcr-plates-low-profile-unskirted-white">MLL4851XTU</a>) PCR Plates, low-profile, unskirted</li> <li class="hidden US-only CA-only AT-only BE-only DK-only FI-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only">PCR 8-Tube Strips without Caps, low-profile, 0.2 ml (<a href="/en-us/sku/tls0851xtu-pcr-tube-strips-without-caps-low-profile-0-2-ml-8-tube-strips">TLS0851XTU</a>) and 8-Cap Strips, optical, flat (<a href="/en-us/sku/tcs0803xtu-pcr-tube-8-cap-strips-optical-flat-8-caps">TCS0803XTU</a>)</li> <li class="hidden US-only CA-only AT-only BE-only DK-only FI-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only">CFX96 Touch&trade; (<a href="/en-us/sku/3600037-cfx96-touch-real-time-pcr-detection-system">3600037</a>) and MiniOpticon&trade; (<a href="/en-us/sku/3591592-miniopticon-real-time-pcr-detection-system">3591592</a>) Real-Time PCR Detection Systems</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/food-pcr-testing-kits">Food PCR Testing Kits</a></li> <li><a href="/en-us/category/iq-check-pcr-automation">iQ-Check PCR Automation</a></li> <li><a href="/en-us/category/chromogenic-media">Chromogenic Media</a></li> <li><a href="/en-us/category/food-science-equipment-supplies">Food Science Equipment and Supplies</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <p>iQ-Check <em>E. coli</em> O157:H7 PCR Detection Kit <a href="/en-us/product/iq-check-e-coli-o157-h7-kit?tab=Documents">Instruction Manuals</a></p> /webroot/web/html/fsd/products/SKUs/autopopulated/ 357-8114_0921121048702_en.html /webroot/web/html/fsd/products/SKUs/autopopulated//357-8114_0921121048702_en.html Assay type 1351 PCR 0 0 Organism 2367 E. coli O157:H7 0 0 3564748 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4748_0921121047608.xml 3564001 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4001_1357810521.xml 3564426 /templatedata/internet/product_SKU/data/FSD/Food-Testing/Chromogenic-Media/356-4426_0921121047629.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578114&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578114&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3578114&quantity= 3578114
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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Click to download
TS_REC_O157:H7_en RAPID'E.coli O157:H7 Agar Technical Sheet, v. 2 (All countries except U.S. and Canada) Click to download
FT_REC O157 H7_fr RAPID’ E. coli O157:H7/Gélose Fiche Technique (Tous pays, sauf USA & Canada) Click to download

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