RAPID'E.coli 2 Medium

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Overview



Key Benefits
  • Simultaneous enumeration of E.coli and coliforms at 37°C without further confirmation
  • Enumeration of E.coli at 44°C without further confirmation
  • Complete results in 18–24 hr
  • Gas production not necessary for differentiation of colonies

Protocol
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/1-07/93
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID'E.coli 2 method is certified by AFNOR Certification according to the ISO 16140 requirements (No BRD 07/1-07/93 and NoBRD 07/7-12/04) and AOAC-RI validated (No. 050601).

Nordval Validation Ref No020.



Intended Use

RAPID'E.coli 2 Medium is a selective chromogenic agar used for direct enumeration, without confirmation, of colonies of Escherichia coli and other coliforms in food products and environmental samples.

Key Benefits

  • Simultaneous enumeration of E. coli and coliforms at 37°C without further confirmation
  • Specific enumeration of E. coli at 44°C without further confirmation
  • Results in 18–24 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Principle

The medium is based on the simultaneous detection of β-D-Glucuronidase (GLUC) and β-D-Galactosidase (GAL) activities. E. coli (GAL+/GLUC+) forms violet to pink colonies, while other coliforms (GAL+/GLUC-) form blue to green colonies.

Detection of GLUC confers a high specificity to the method because E. coli is one of the few species of Enterobacteriaceae to possess this enzyme. Growth of interfering flora is inhibited, especially at 44°C, conferring high specificity to the medium.

RAPID'E.coli 2 Chromogenic Assay

Enzymatic Activity
E. coli

Other coliforms

Non-coliforms
Glucuronidase + - -
Galactosidase + + -
       

Two Easy Protocols

  • A single layer and a single plate per dilution for counting colonies of E. coli at 44°C
  • Counting colonies of E. coli and coliforms at 37°C within a single layer on the same plate

Ordering Information

RAPID'E.coli 2 Medium is available as a ready-to-use agar broth (3555299) or in dehydrated form (3564024).

Assay type Chromogenic media
Organism E. coli, coliforms
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RAPID'E.coli 2 Agar, ready to use #355-5299
RAPID'E.coli 2 Agar, ready to use

3555299
Pkg of 6, 100 ml bottles

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$32.00
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RAPID'E.coli 2 Agar, dehydrated #356-4024
RAPID'E.coli 2 Agar, dehydrated

3564024
Pkg of 1, 500 g of dehydrated agar broth, enough for 13.5 L of medium

List Price:
 
$370.00
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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
PF_260_fr Gélose RAPID’E.coli 2 Information Produit (Tous pays, sauf USA & Canada) Click to download
TS_REC2 RAPID'E.coli 2 Agar Technical Sheet (U.S. and Canada only) Click to download
TS_REC2_en RAPID'E.coli 2 Agar Technical Sheet (All countries except U.S. and Canada) Click to download
FT_REC2_fr RAPID’E.coli 2/Gélose Fiche Technique (Tous pays, sauf USA & Canada) Click to download