RAPID'E.coli 2 Medium

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Overview



Key Benefits
  • Simultaneous enumeration of E.coli and coliforms at 37°C without further confirmation
  • Enumeration of E.coli at 44°C without further confirmation
  • Complete results in 18–24 hr
  • Gas production not necessary for differentiation of colonies

Protocol
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/1-07/93
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID'E.coli 2 method is certified by AFNOR Certification according to the ISO 16140 requirements (No BRD 07/1-07/93 and NoBRD 07/7-12/04) and AOAC-RI validated (No. 050601).

Nordval Validation Ref No020.

Intended Use

RAPID’E.coli 2™ medium is a selective chromogenic agar used for direct enumeration, without confirmation of colonies, of Escherichia coli and other coliforms in food products and environmental samples (depth method).

High Specificity

The medium is based on simultaneous detection of

  • β-D-Glucuronidase (β GLUC)
  • β-D-Galactosidase (β GAL)

E.coli (GAL+/GLUC+) forms violet to pink colonies; other coliforms (GAL+/GLUC–) form blue to green colonies. Detection of GLUC confers a high specificity to the culture medium. E. coli is one of the only species of Enterobacteriaceae to possess this enzyme.

Most interfering flora are inhibited, especially at 44°C, giving the medium high specificity.

Two Easy Protocols

  • A single layer and a single plate per dilution for enumeration of E.coli at 44°C
  • Enumeration of E.coli and coliforms at 37°C with a single layer on the same plate
Assay type Chromogenic media
Organism E. coli.coliforms.
RAPID'E.coli 2 Agar, ready to use #355-5299
RAPID'E.coli 2 Agar, ready to use

355-5299
100 ml x 6 bottles

List Price:   $28.00
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RAPID'E.coli 2 Agar, dehydrated #356-4024
RAPID'E.coli 2 Agar, dehydrated

356-4024
500 g

List Price:   $370.00
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Number Description Options
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Brochure, Rev H Click to download
FSD_17049 RAPID’E.coli 2 Agar Product Information Click to download
TS_REC2_en RAPID'E.coli 2 Agar Technical Sheet (All countries except U.S. and Canada) Click to download
FT_REC2_fr RAPID’E. coli 2/Gélose Fiche Technique (Tous pays, sauf USA & Canada) Click to download
TS_REC2 RAPID'E.coli 2 Agar Technical Sheet (U.S. and Canada only) Click to download