QX200 ddPCR EvaGreen Supermixes

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QX200 ddPCR EvaGreen Supermixes

The QX200 EvaGreen digital PCR supermix is a 2x concentrated, ready-to-use universal mix that delivers maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).

This supermix supports double-stranded DNA target detection following amplification using commercially available EvaGreen assays.

Key Benefits of the QX200 ddPCR EvaGreen Supermix

  • Contains all components required for EvaGreen-based ddPCR except primers and template
  • Enables double-stranded DNA detection — only primers are needed to amplify and detect product
  • Optimized for use with validated Bio-Rad’s PrimePCR™ assays
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses of the QX200 ddPCR EvaGreen Supermix

  • Absolute quantification
  • Gene expression analysis
  • Copy number detection
  • DNA rearrangements
  • Multiplexing assays

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-4033
500 5 x 1 186-4034
2,500 5 x 5 186-4035
5,000 10 x 5 186-4036


Related Products

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

These digital PCR assays require the use of the following instruments and accessories:

We also recommend using:

More Information

The QX200 ddPCR EvaGreen supermix is optimized for use with droplet generation oil for EvaGreen on the QX200™ Droplet Digital™ PCR system.

Product expiration (after shipping date) 12 months
Storage at –20°C (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 2 weeks
Customer guarantee (minimum usage time) at –20°C 12 months
QX200™ ddPCR™ EvaGreen Supermix

186-4033
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR system

List Price:   $164.00
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QX200™ ddPCR™ EvaGreen Supermix

186-4034
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR system

List Price:   $410.00
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QX200™ ddPCR™ EvaGreen Supermix

186-4035
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR system

List Price:   $2,050.00
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QX200™ ddPCR™ EvaGreen Supermix

186-4036
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR system

List Price:   $4,100.00
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Number Description Options
10040717 QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev B Click to download
6450 Droplet Digital PCR Publications List, Rev E Click to download
10028376 QX200® EvaGreen® ddPCR® Supermix Click to download
6473 QX200 ddPCR EvaGreen Supermix Flier, Rev A Click to download
6311 QX200 Droplet Digital PCR System Brochure, Rev B Click to download
10031907 Instruction Manual, QX200 Droplet Generator, Rev A Click to download
10031906 Instruction Manual, QX200 Droplet Reader and QuantaSoft Software, Rev A Click to download