QX200™ ddPCR™ EvaGreen Supermix

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QX200 ddPCR EvaGreen Supermixes

This QX200 EvaGreen Digital PCR Supermix is a 2x concentrated, ready-to-use universal mix that delivers maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).

This supermix supports double-stranded DNA target detection following amplification using commercially available EvaGreen Assays.

Key Benefits

  • Contains all components required for EvaGreen-based ddPCR except primers and template
  • Enables double-stranded DNA detection — only primers are needed to amplify and detect product
  • Optimized for use with Bio-Rad’s PrimePCR™ Assays
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses

  • Absolute quantification
  • Gene expression analysis
  • Copy number detection
  • DNA rearrangements
  • Multiple target analysis

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-4033
500 5 x 1 186-4034
2,500 5 x 5 186-4035
5,000 10 x 5 186-4036

Additional Reagents

In addition to the QX200™ ddPCR™ EvaGreen Supermix, we offer these consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

The QX200 ddPCR EvaGreen Supermix is optimized for use with Droplet Generation Oil for EvaGreen on the QX200™ Droplet Digital™ PCR System.

Storage at –20°C Up to 12 months (Refer to expiration date)
Storage at 4°C (after thawing) Up to 2 weeks
Template compatibility cDNA, genomic DNA, plasmid DNA
QX200™ ddPCR™ EvaGreen Supermix

186-4033
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

List Price:   $164.00
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QX200™ ddPCR™ EvaGreen Supermix

186-4034
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

List Price:   $410.00
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QX200™ ddPCR™ EvaGreen Supermix

186-4035
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

List Price:   $2,050.00
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QX200™ ddPCR™ EvaGreen Supermix

186-4036
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x supermix, for use in sample preparation with the droplet generator in the QX200™ Droplet Digital™ PCR System

List Price:   $4,100.00
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Number Description Options
6473 QX200 ddPCR EvaGreen Supermix Flier, Rev A Click to download
10028376 Instruction Manual, QX200 EvaGreen ddPCR Supermix, Rev B Click to download
10040717 QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev B Click to download
6311 QX200 Droplet Digital PCR System Brochure, Rev B Click to download
6450 Droplet Digital PCR Publications List, Rev F Click to download
10031907 Instruction Manual, QX200 Droplet Generator, Rev A Click to download
10031906 Instruction Manual, QX200 Droplet Reader and QuantaSoft Software, Rev A Click to download