Pulsed Field Standards and Agaroses

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Overview

Introduction to Pulsed Field Gel Electrophoresis

This is from Product Overlay
PFGE Standards Selection Guide

Comparison of Pulsed Field Standards

  Range Contents Amount Number of Applications
5 kb ladder 4.9–120 kb Concatemers of pBR328 20 µg in 200 µl 20–25
8–48 kb ladder 8.3–48.5 kb Mixed digest of phage λ 25 µg in 125 µl 125
Lambda(λ) ladder 0.05–1 Mb Concatemers of phage λcl857Sam7 5 agarose blocks 25–40
S. cerevisiae 0.225–2.2 Mb Saccharomyces cerevisiae chromosomal DNA 5 agarose blocks 25–40
H. wingei 1–3.1 Mb Hansenula wingei chromosomal DNA 5 agarose blocks 25–40
S. pombe 3.5–5.7 Mb Schizosaccharomyces pombe chromosomal DNA 5 agarose blocks 25–40
Certified Agarose Selection Guide
 
Packaging
25 g 161-3100 161-3103
161-3106   161-3111 161-3113 162-0137
100 g             162-0138
125 g 161-3101 161-3104 161-3107   161-3112 161-3114  
500 g 161-3102 161-3105       161-3115  
Analytical Separation
≥1,000 bp          
≤1,000 bp          
20–800 bp            
1 kb–2 Mb          
1 kb–5 Mb            
Preparative Separation
Quantum Prep methods      
Low-melt methods          
Agarose methods          
In-gel applications          
Other Applications
Postpreparative enzymatic treatments    
Tissue/cell culture            
Pulsed field sample preparation          
Blotting      

Certified megabase agarose — With very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA. Certified megabase agarose has superior electrophoretic mobility, faster migration rates, faster running times, and better separation of megabase fragments than pulsed field Certified agarose.

Pulsed field Certified™ agarose — This agarose enables excellent separation and resolution of large DNA in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified megabase agarose, running conditions for this agarose are a preset selectable method of the CHEF Mapper XA system auto-algorithm.

Pulsed field standards — Standards for pulsed field applications save days of preparation time. Bio-Rad offers standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. The higher molecular weight standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions. Choose from recombinant DNA ladders or genomic digests.

5 kb Ladder  
Range 4.9–120 kb
Contents Concatemers of pBR328 partially digested with EcoRI
Supplied in  Pulsed field Certified agarose
Quantity 20 µg
Concentration 0.1 µg/µl
Number of applications 20–25
8–48 kb Ladder  
Range 8.3–48.5 kb
Contents Mixed digest of phage λ
Supplied in Pulsed field Certified agarose 
Quantity 25 µg
Concentration 0.2 µg/µl
Number of applications 125
Lambda (λ) Ladder  
Range 0.05–1 Mb
Contents Concatemers of phage λ c1857 Sam7
Supplied in Pulsed field Certified agarose
Quantity 5 agarose blocks
Concentration 15 µg per insert
Number of applications  25–40
S. cerevisiae Size Marker  
Range 0.225–2.2 Mb
Contents Saccharomyces cerevisiae chromosomal DNA
Supplied in  Pulsed field Certified agarose
Quantity  5 agarose blocks
Concentration 1.75 x 108 cells/insert
Number of applications 25–40
H. wingei Size Marker  
Range 1–3.1 Mb
Contents   Hansenula wingei chromosomal DNA
Supplied in    Pulsed field Certified agarose
Quantity 5 agarose blocks 
Number  of applications 25–40
S. pombe Size Marker  
Range 3.5–5.7 Mb
Contents Schizosaccharomyces pombe chromosomal DNA
Supplied in Pulsed field Certified  agarose
Quantity    5 agarose blocks 
Concentration 2.25 x 108 cells/insert
Number of applications 25–40
Certified Megabase Agarose

161-3108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:   $85.00
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Certified Megabase Agarose

161-3109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:   $276.00
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Certified Megabase Agarose

161-3110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

List Price:   $871.00
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Certified Molecular Biology Agarose

161-3100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $59.00
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Certified Molecular Biology Agarose

161-3101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $169.00
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Certified Molecular Biology Agarose

161-3102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

List Price:   $543.00
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Pulsed Field Certified Agarose

162-0137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

List Price:   $215.00
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Pulsed Field Certified Agarose

162-0138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

List Price:   $984.00
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CHEF DNA Size Marker

170-3605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs

List Price:   $143.00
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CHEF DNA Size Marker

170-3667
5 agarose blocks, PFGE marker, 1–3.1 Mb, H. wingei chromosomal DNA, for 25–40 plugs

List Price:   $153.00
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CHEF DNA Size Marker

170-3633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs

List Price:   $153.00
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CHEF DNA Size Standard

170-3624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications

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CHEF DNA Size Standard

170-3707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications

List Price:   $85.00
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CHEF DNA Size Standard

170-3635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 0.05–1 Mb, concatemers of phage λcl857Sam7, for 25–40 plugs

List Price:   $400.00
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Number Description Options
5901 Pulsed Field Gel Electrophoresis Brochure, Rev B Click to download [ Add to Cart (Free) ]
LIT388 Instruction Manual, CHEF DNA Size Marker, S. pombe, Rev C Click to download
LIT291 Instruction Manual, CHEF DNA Size Standards, 5 kb Ladder, Rev B Click to download
LIT538 Instruction Manual, CHEF DNA Size Marker, H. wingei, Rev B Click to download
LIT299 Instruction Manual, CHEF DNA Size Marker, S. cerevisiae, Rev E Click to download
LIT556 Instruction Manual, CHEF DNA Size Standards, 8–48 kb, Rev B Click to download
LIT298 Instruction Manual, CHEF DNA Size Standards, Lambda Ladder, Rev C Click to download [ Add to Cart (Free) ]