Pulsed Field Standards

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Overview

Specifications
5 kb ladder  
Range 4.9–120 kb
Contents Concatemers of pBR328 partially digested with EcoRI
Supplied in 28mM Tris, pH 8.0, 50mM EDTA, 28 mM NaCl, 0.05% SDS
Quantity 20 µg
Concentration 0.1 µg/µl
Number of applications 20–25
8–48 kb ladder  
Range 8.3–48.5 kb
Contents Mixed digest of phage λ
Supplied in TE (10 mM Tris-HCL, pH 8.0, 1.0 mM EDTA, pH 8.0)
Quantity 25 µg
Concentration 0.2 µg/µl
Number of applications 125
Lambda (λ) ladder  
Range 0.05–1 Mb
Contents Concatemers of phage λ cl857Sam7 embedded in 1%
Supplied in 1% Bio-Rad's Low Melt Agarose
Quantity 5 agarose blocks
Concentration 15 µg per insert
Number of applications 25–40
S. cerevisiae size marker  
Range 0.225–2.2 Mb
Contents Saccharomyces cerevisiae chromosomal DNA
Supplied in 1% Bio-Rad's Low Melt Agarose
Quantity 5 agarose blocks
Concentration 1.75 x 108 cells/insert
Number of applications 25–40
H. wingei size marker  
Range 1–3.1 Mb
Contents Hansenula wingei chromosomal DNA
Supplied in 0.75% Bio-Rad's Low Melt Agarose
Quantity 5 agarose blocks
Concentration  
Number of applications 25–40
S. pombe size marker  
Range 3.5–5.7 Mb
Contents Schizosaccharomyces pombe chromosomal DNA
Supplied in 1% Bio-Rad's Low Melt Agarose
Quantity 5 agarose blocks
Concentration 2.25 x 108 cells/insert
Number of applications 25–40
PFGE Standards Selection Guide
5 kb Ladder 8–48 kb Ladder Lambda Ladder S. cerevisiae
Chromosomal DNA
H. wingei
Chromosomal DNA
S. pombe
Chromosomal DNA
Comparison of Pulsed Field Standards
 
  Range Contents Amount Number of Applications
 
5 kb ladder 4.9–120 kb Concatemers of pBR328 20 µg in 200 µl 20–25
8–48 kb ladder 8.3–48.5 kb Mixed digest of phage λ 25 µg in 125 µl 125
Lambda (λ) ladder 0.05–1 Mb Concatemers of phage λcl857Sam7 5 agarose blocks 25–40
S. cerevisiae 0.225–2.2 Mb Saccharomyces cerevisiae chromosomal DNA 5 agarose blocks 25–40
H. wingei 1–3.1 Mb Hansenula wingei chromosomal DNA 5 agarose blocks 25–40
S. pombe 3.5–5.7 Mb Schizosaccharomyces pombe chromosomal DNA 5 agarose blocks 25–40
 

 

Bio-Rad standards for pulsed field applications can be used for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. The higher molecular weight standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions. Choose from recombinant DNA ladders or genomic digests.

CHEF DNA Size Marker

170-3605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs

List Price:   $143.00
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CHEF DNA Size Standard

170-3624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications

List Price:   $199.00
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CHEF DNA Size Marker

170-3633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs

List Price:   $153.00
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CHEF DNA Size Standard

170-3635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 0.05–1 Mb, concatemers of phage λcl857Sam7, for 25–40 plugs

List Price:   $400.00
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CHEF DNA Size Marker

170-3667
5 agarose blocks, PFGE marker, 1–3.1 Mb, H. wingei chromosomal DNA, for 25–40 plugs

List Price:   $153.00
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CHEF DNA Size Standard

170-3707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications

List Price:   $85.00
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