ProteOn™ XPR36 Protein Interaction Array System

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Overview

ProteOn Technology and Applications ProteOn Technology and Applications
ProteOn™ Technology and Applications
Analysis of Lipid-Protein Interactions
Analysis of Lipid-Protein Interactions
Analysis of Integral Membrane Protein–Antibody and Lipid-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System

Introduction
The interplay of interactions between membranes, membrane proteins, and antibodies is of great interest in the field of drug discovery and development. Membrane proteins and membrane-associated proteins and peptides have been shown to be important in many biological processes and are involved in key pathways in diseases such as cancer. Membrane proteins are fast becoming the next big class of drug targets.

This poster describes the use of a ProteOn XPR36 protein interaction array system, which uses surface plasmon resonance to measure 36 biomolecular interactions simultaneously, to study the interaction of artificial membranes and two significant peptides.

PDF Bulletin 5922 To read more click here

Bibliography
ProteOn XPR36 Protein Interaction Array System Bibliography
ProteOn Webinar Series ProteOn Webinar Series
ProteOn™ Webinar Series

The ProteOn webinars feature presentations from leaders in the field of label-free biomolecular interaction analysis using surface plasmon resonance (SPR) technology. Since 2009 this webinar series has covered a wide range of novel SPR applications spanning protein characterization, antibody and small molecule drug discovery, and structural biology research topics.

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The ProteOn™ XPR36 protein interaction array system is an SPR optical biosensor that provides real-time data on the affinity, specificity, and interaction kinetics of protein interactions. Using XPR technology*, a unique approach to multiplexing, this system generates a 6 x 6 interaction array for the simultaneous analysis of up to six ligands with up to six analytes. The ProteOn XPR36 system increases the throughput, flexibility, and versatility of experiment design, enabling the completion of more experiments in less time:

  • Analyze up to 36 different protein interactions in a single run, on a single chip
  • Perform a complete kinetic analysis in a single run
  • Measure a variety of experimental conditions simultaneously in parallel
  • Screen multiple panels of analytes

See the ProteOn Technology and Applications videos for more information about how the ProteOn system works and how it can be applied to drug discovery and to address important experimental questions.

The ProteOn XPR36 protein interaction analysis system includes all of the components required for interaction analysis: instrumentation, ProteOn Manager™ software, ProteOn sensor chips, buffer and reagent kits, and protocol development kits.

User-friendly ProteOn Manager software uses a flexible, guided approach to coordinate instrument control, experiment setup, and data analysis.

Key Features and Benefits

  • Novel 6 x 6 array design
  • Real-time data acquisition
  • Analysis of interaction kinetics, binding affinity, and analyte concentration
  • No radiochemical or fluorescent labels needed
  • Integrated system

Applications and Uses

  • Antibody characterization and development
  • Large and small molecule drug development
  • Protein interface analysis
  • Protein complex and cascade analysis

*The ProteOn XPR36 protein interaction array system is covered by Bio-Rad patents, including United States patent numbers 8,111,400, 8,105,845, 7,999,942, and 7,443,507.

This product or portions thereof is manufactured and sold under license from GE Healthcare under United States patent numbers 5,554,541, 7,736,587, and 8,021,626, and any international patents and patent applications claiming priority.

Number of interaction spots 36
Number of interspot references 42
Response uniformity <2% CV
Refractive index range 1.33–1.37 refractive index units
Dynamic range, RU 1–40,000
Baseline noise <1 RU, 1–20,000 RU
<1.5 RU, 20,000–40,000 RU
Baseline drift <1 RU/min (15–40°C)*
Operating temperature range 15–40°C*
Autosampler temperature range 2–35°C
Sample configuration 72 x 1.5 ml vials or 2 x 96-well microplates
CCD 12-bit digital camera
Acquisition rate 3 Hz (3 images/sec), average 3 images/data point
Weight, kg 85
Dimensions (W x H x D), cm 95 x 58 x 50
Controller with 19" flat panel monitor (1,280 x 1,024 pixels) supplied with instrument  
* Not lower than 15°C below room temperature.  
ProteOn XPR36 Protein Interaction Array System #176-0100
ProteOn XPR36 Protein Interaction Array System

176-0100
100–240 V, includes ProteOn XPR36 instrument, ProteOn Manager™ software, controller/display, maintenance kit (#176-4100), One-shot Kinetics™ kit (#176-1010), buffers, and accessories

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ProteOn Controller and Display #176-0300
ProteOn Controller and Display

176-0300
PC and 19 in. flat-panel monitor (1,280 x 1,024 pixels), for use with the ProteOn XPR36 protein interaction array system

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ProteOn Manager Software #176-0200
ProteOn Manager™ Software

176-0200
Software for ProteOn XPR36 instrument control, experiment design, data collection, and analysis, CD-ROM, includes HASP key

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ProteOn Sample Rack

176-6000
Pkg of 1, rack for 72 ProteOn sample vials (1.5 ml); for use with the ProteOn XPR36 protein interaction array system

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ProteOn Sample Rack Needle Arm

176-6001
Pkg of 1, needle holder for rack autosampler layout and needle configuration in the ProteOn XPR36 system; accommodates ProteOn sample rack

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ProteOn Microplate Needle Arm

176-6002
Pkg of 1, needle holder for microplate autosampler layout and needle configuration in the ProteOn system; accommodates standard and deep well microplates

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ProteOn Needles

176-6003
Pkg of 6, needle, accommodates the ProteOn sample rack and ProteOn microplate needle holders

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ProteOn Wash Station

176-6004
Pkg of 1, needle wash station, for use with ProteOn XPR36 protein interaction array system

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ProteOn Running Buffer Bottle

176-2700
Pkg of 1 bottle, holds 2 L running buffer

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ProteOn Communication Cable

176-0315
Pkg of 1, cable used to connect the controller to the ProteOn XPR36 protein interaction array instrument

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ProteOn XPR36 Buffer Inlet Filters

176-4114
Pkg of 2, buffer inlet filters for use with the ProteOn XPR36 protein interaction array system

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ProteOn Syringe

176-6005
Pkg of 1, replacement syringe for use with the ProteOn XPR36 protein interaction array system

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ProteOn Syringes, set of 6

176-6050
Pkg of 6, replacement syringes for use with the ProteOn XPR36 protein interaction array system

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ProteOn Collection Tank

176-6060
Pkg of 1, 10 L tank to collect buffer and solution overflow from the ProteOn XPR36 protein interaction array system

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ProteOn Collection Tank Tubing

176-6061
Pkg of 1, 1.75 m tubing to connect the ProteOn collection tank (#176-6060) to the ProteOn XPR36 protein interaction array system

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Number Description Options
6550 Building New Anti-HIV Molecules with Bio-Rad’s ProteOn™ XPR36 System, Rev A Click to download
6132 The ProteOn HTG Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Protein Interaction Analysis, Rev A Click to download
6299 Guide to SPR Data Processing on the ProteOn XPR36 Protein Interaction Assay System, Rev A Click to download
6300 Guide to SPR Data Analysis on the ProteOn XPR36 System, Rev A Click to download
6295 ProteOn Sensor Chips, Tips and Techniques, Rev A Click to download
5948 Attachment of Membrane Protein Interactions Using Lipoparticles Bound to the Surface of the ProteOn XPR36 Sensor Chips, Rev A Click to download [ Add to Cart (Free) ]
5968 Screening and Characterization of Human Immunoglobulin G Binding Peptides Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download [ Add to Cart (Free) ]
6044 Analyte Interaction and Kinetics Guide, Rev A Click to download
5960 High-Throughput Profiling of Kinase Inhibitors Selectivity Using the ProteOn™ XPR36 Protein Interaction Array System, Rev A Click to download
5390 ProteOn XPR36 Protein Interaction Array System Brochure, Rev D Click to download [ Add to Cart (Free) ]
5538 Analyzing Protein Interactions With the ProteOn XPR36 Protein Interaction Array System, Rev D Click to download [ Add to Cart (Free) ]
5413 ProteOn XPR36 Hardware Product Information Sheet, Rev B Click to download
5627 ProteOn Manager Software Product Information Sheet, Rev C Click to download [ Add to Cart (Free) ]
5404 ProteOn Sensor Chips Product Information Sheet, Rev E Click to download [ Add to Cart (Free) ]
5410 ProteOn Protocol Development Kits Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5409 Protein Interaction Analysis Ordering Information Sheet, Rev E Click to download [ Add to Cart (Free) ]
5819 ProteOn XPR36 Protein Interaction Array System: Regulatory Tools for Drug Development Product Focus, Rev A Click to download
5965 Rapid, High-Throughput Screening of Protein Kinase Inhibitors Using the ProteOn XPR36 Protein Interaction Array System, Rev B Click to download
5846 Determining the Binding Kinetics of HIV-1 Nucleocapsid Protein to Six Densities of Oligonucleotide Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
5412 Antibody Characterization and Development: ProteOn XPR36 Protein Interaction Array System Application Guide, Rev C Click to download
5540 Screening, Ranking, and Epitope Mapping of Anti-Human IL-9 Supernatants, Rev A Click to download
5820 Rapid Screening and Selection of Optimal Antibody Capturing Agents Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download [ Add to Cart (Free) ]
3172 Rapid and Efficient Determination of Kinetic Rate Constants Using the ProteOn XPR36 Protein Interaction Array System, Rev B Click to download
5360 Rapid and Detailed Analysis of Multiple Antigen-Antibody Pairs Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
5367 Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
5368 Anaylsis of Multiple Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev B Click to download
5358 Mechanisms of Protein-Protein Binding: Double-Mutant Cycle Analysis Using the ProteOn XPR36 System, Rev A Click to download
5821 Guide to Ligand Immobilization Protocol Guide, Rev A Click to download
5822 How to Perform Excluded Volume Correction on the ProteOn XPR36 Protein Interaction System Protocol Guide, Rev C Click to download
5797 Rapid Assay Development and Optimization for Small Molecule Drug Discovery, Rev B Click to download [ Add to Cart (Free) ]
5679 Applications of the ProteOn GLH Sensor Chip: Interactions Between Proteins and Small Molecules, Rev A Click to download [ Add to Cart (Free) ]
5449 Applications of the ProteOn NLC Sensor Chip: Antibody-Antigen, DNA-Protein, and Protein-Protein Interactions, Rev B Click to download [ Add to Cart (Free) ]
5434A ProteOn XPR36 Protein Interaction Array System Folder. (Contains bulletins 3172, 5358, 5360, 5367, 5368, 5390, 5404, 5409, 5410, 5413, 5538, and 5465 DVD, depending on availability) [ Add to Cart (Free) ]
6161 Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download [ Add to Cart (Free) ]
6254 The ProteOn HTE Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Small Molecule Interaction Analysis, Rev A Click to download [ Add to Cart (Free) ]
6449 Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction using the ProteOn XPR36 System, Rev A Click to download
6414 ProteOn XPR36 Experimental Design and Application Guide, Rev A Click to download