ProteOn protocol development kits provide the reagents and sensor chips needed to perform and analyze data from a complete experiment using the ProteOn XPR36 protein interaction array system. New users will gain familiarity with instrument setup and operation, experiment design, and data analysis. Experienced users will find the kits valuable for system benchmarking and protocol development.
ProteOn One-shot Kinetics Kit
The interaction between the cytokine IL-2 and an IL-2 antibody demonstrates a detailed kinetic analysis in a single injection cycle (One-shot Kinetics). The kit also demonstrates a useful method for controlling ligand immobilization levels during protocol development and optimization.
The ProteOn One-shot Kinetics kit includes a ProteOn IL-2/IL-2 antibody pair, with sufficient materials to immobilize IL-2 antibody in up to six channels and to analyze a complete concentration series of IL-2 across each channel.
ProteOn Multiple Protein Interaction Kit
The interaction between five mutant TEM1 β-lactamase proteins and wild-type β-lactamase inhibitor protein(BLIP) demonstrates the power of the ProteOn XPR36 system to produce a detailed kinetic analysis of multiple simultaneous interactions and to map protein interfaces.
The ProteOn multiple protein interaction kit includes a ProteOn TEM1/BLIP protein set, with sufficient materials to immobilize five TEM1 mutant proteins in five channels and to perform a detailed kinetic analysis of the interaction of each of the five mutant proteins with BLIP.
ProteOn Protein-Small Molecule Kit
The interaction between carbonic anhydrase II and 4-carboxy benzene sulfonamide (4-CBS) demonstrates the capability of the ProteOn XPR36 system to detect low molecular weight analytes. The kit also provides a benchmark for optimal system performance.
The ProteOn protein-small molecule kit includes ProteOn carbonic anhydrase II/CBS, with sufficient materials to immobilize carbonic anhydrase II in up to six channels and analyze a complete concentration series of CBS.