ProteinChip Profiling Kits

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Overview

ProteinChip Array Selection Guide
General Description Array Surface Chemistry (active spots) Applications
Strong Anion Exchange
Capture of molecules that have negative surface charges Q10 Cationic, quaternary ammonium groups that interact with the negative charges on the surface of target proteins; for example, aspartic or glutamic acid
  • Selective analysis of proteins with low pls
  • Biomarker discovery
Weak Cation Exchange
Capture of molecules that have positive surface charges CM10 Weak anionic carboxylate groups that interact with the positive charges on the surface of the analyte; for example, lysine, arginine, or histidine
  • Selective analysis of proteins with high pls
  • Biomarker discovery
IMAC
Capture of molecules that bind polyvalent metal ions, such as nickel, copper, zinc, iron, and gallium IMAC30 NTA groups that chelate metal ions. Proteins applied to the array surface may bind to the chelated metal ion through histidine, tryptophan, cysteine, and phosphorylated amino acid residues
  • Analysis of metal-binding, phosphorylated, or polyhistidine (His)-tagged proteins
  • Biomarker discovery
Hydrophobic/Reverse-Phase
Capture of large proteins through hydrophobic or reverse-phase interactions H50 Methylene chains that closely mimic the characteristics of C6 to C12 alkyl chromatography media
  • Protein profiling
  • Purity determination
  • Rapid protein analysis
  • Calibration with proteins of known MW
  • Biomarker discovery
Capture of smaller proteins and peptides through hydrophobic and reverse-phase interactions H4* Active spots contain chains of 16 methylene groups that bind proteins through reverse-phase chemistry. Binds proteins abundant in alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, or tyrosine
  • Purity determination
  • Rapid protein/peptide analysis
  • Biomarker discovery
Analysis of peptides resulting from tryptic digestion of proteins SEND ID Chains of 18 methylene groups (C18) that bind peptides through reverse-phase chemistry; matrix is integrated into the array surface
  • Peptide mass fingerprinting
Normal Phase
General protein binding surface; recommended for hydrophilic proteins NP20 Silicon dioxide, which allows proteins to bind via serine, threonine, or lysine residues
  • Quick protein analysis
  • Purity determination
  • Verification of the presence or absence of a molecule
  • Calibration with peptides or proteins of known MW
Preactivated Surface
Covalent immobilization of biomolecules for subsequent specific capture of proteins from complex samples PS10 Reactive acyl imidazole moieties
  • Analysis of antibody-antigen, receptor-ligand, protein-protein interactions
  PS20 Epoxy groups  
  RS100 Reactive acyl imidazole moieties on a hydrogel backbone  
Nonreactive Surface
  Au Gold provides a nonreactive surface for general protein analysis (limited reusability)
  • Quick protein analysis
  • Calibration with peptides or proteins of known MW

*Array lacks the hydrophobic coating needed for sample containment; a hydrophobic PAP pen can be used to manually create the barrier.

SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

Request a Biomarker Discovery Guide

Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

  • Guidelines for proper sample selection, collection, handling, and storage
  • Instructions for planning the experimental workflow
  • Tips on improving reproducibility through standard operating procedures
  • Important considerations for data analysis implementation
  • Optimized methods and protocols for serum or plasma analysis


The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

  • General data analysis workflows
  • Data organization
  • Data processing steps, including baseline subtraction, normalization, and spectrum alignment
  • Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis
  • Glossary of software terms

ProteinChip buffer and array kits provide all of the necessary reagents and ProteinChip arrays needed to generate accurate results with minimal training time. Three ProteinChip profiling kits are available, providing the surfaces routinely used in profiling experiments: reverse-phase (H50), metal affinity capture (IMAC30), and weak cation exchange (CM10). The binding and washing buffers provided in the kits have been optimized to bind the maximum amount of proteins, thereby increasing the probability of biomarker discovery.

Benefits of the profiling kits include

  • Improved results — optimized binding buffers increase the probability that new biomarkers will be discovered
  • Proven protocols — supplied with the kits, these have been validated by researchers working on a wide range of applied research projects
  • Convenience — buffers are premade to save time
  • Flexibility — kits can be used manually or with automation
  • Consistency — buffers are pH-tested to ensure they are within 0.05 of the specified pH for reproducible results; an antimicrobial preservative ensures reliable performance
  • Ease of use — step-by-step protocols are provided with kits

Refer to the ProteinChip Array Selection Guide for more information on selecting the appropriate array for your biomarker experiment.

ProteinChip CM Kit

K20-30003
Kit contains 12 ProteinChip CM10 arrays and ProteinChip CM low-stringency buffer

List Price:   $1,129.00
Quantity:   Add to Quote
This product is not available for sale online. Please contact customer service for more information.
 
ProteinChip H50 Kit

K20-30001
Kit contains 12 ProteinChip H50 arrays and ProteinChip H50 buffer

List Price:   $1,129.00
Quantity:   Add to Quote
This product is not available for sale online. Please contact customer service for more information.
 
Hazardous  
Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download