ProteinChip Matrices

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Overview

ProteinChip Matrix Selection Guide
Protein CHCA SPA EAM-1
<30 kD
10–150 kD
Glycosylated; 15–50 kD
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Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

  • Guidelines for proper sample selection, collection, handling, and storage
  • Instructions for planning the experimental workflow
  • Tips on improving reproducibility through standard operating procedures
  • Important considerations for data analysis implementation
  • Optimized methods and protocols for serum or plasma analysis


The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

  • General data analysis workflows
  • Data organization
  • Data processing steps, including baseline subtraction, normalization, and spectrum alignment
  • Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis
  • Glossary of software terms

SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

After samples are applied to an array and the array is washed, an energy-absorbing matrix (EAM) is applied to each spot to facilitate desorption and ionization. A variety of ProteinChip matrices are available for various applications:

  • Alpha-cyano-4-hydroxycinnamic acid (CHCA) — enables efficient laser desorption and ionization of small proteins (<30 kD)
  • Sinapinic acid (SPA) — enables efficient laser desorption and ionization of larger proteins (10–150 kD)
  • EAM-1 (proprietary formulation) — enables efficient laser desorption and ionization of glycosylated proteins and proteins in the 15–50 kD range
ProteinChip SPA Matrix

C30-00002
Pkg of 20 vials, 5 mg each of sinapinic acid (SPA), for use with ProteinChip arrays

List Price:   $346.00
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ProteinChip EAM-1 Matrix

C30-00003
Pkg of 20 vials, 5 mg each of EAM-1 matrix, for use with ProteinChip arrays

List Price:   $420.00
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ProteinChip CHCA Matrix

C30-00001
Pkg of 20 vials, 5 mg each of α-cyano-4-hydroxycinnamic acid (CHCA), for use with ProteinChip arrays

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ProteinChip Matrix Kit

C30-00004
Pkg of 18 vials, 6 each of α-cyano-4-hydroxycinnamic acid (CHCA), sinapinic acid (SPA), and EAM-1, for use with ProteinChip arrays

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Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download
10008246 Instruction Manual, ProteinChip Energy Absorbing Molecules (EAM), Rev E Click to download