ProteinChip Fractionation and Purification Products

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SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

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Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

  • Guidelines for proper sample selection, collection, handling, and storage
  • Instructions for planning the experimental workflow
  • Tips on improving reproducibility through standard operating procedures
  • Important considerations for data analysis implementation
  • Optimized methods and protocols for serum or plasma analysis


The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

  • General data analysis workflows
  • Data organization
  • Data processing steps, including baseline subtraction, normalization, and spectrum alignment
  • Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis
  • Glossary of software terms

ProteinChip fractionation and purification products are used for applications before and after sample analysis on ProteinChip arrays. Before analysis, they can be used to fractionate a sample to reduce its complexity and increase the likelihood of identifying low-abundance proteins. After analysis and biomarker identification, these products, which have chromatographic properties that mimic the binding and elution characteristics of ProteinChip arrays, can be used to purify proteins discovered on array surfaces.

ProteinChip Spin Columns
ProteinChip spin columns are used for small-scale purification of proteins using a microcentrifuge.

  • ProteinChip Q spin columns — anion exchange spin columns that fractionate samples based on pI through a quaternary amine functionality
  • ProteinChip CM spin columns — cation exchange spin columns that fractionate samples based on pI through a carboxymethyl functionality
  • ProteinChip IMAC spin columns — used for purifying recombinant polyhistidine-tagged proteins (when loaded with Ni2+) or phosphorylated proteins and peptides (when loaded with Ga3+ or Fe3+); IMAC sorbent chelates metal ions based on iminodiacetic acid functionality

 

ProteinChip Q Filtration Plate
The ProteinChip Q filtration plate is a 96-well filtration plate filled with Q ceramic sorbent for sample preparation and purification using a vacuum manifold. The plate separates proteins by anion exchange, based on pI, using a quaternary amine functionality. Fractionation with the ProteinChip Q filtration plate facilitates the analysis of complex biological samples by enriching low-abundance proteins prior to analysis with ProteinChip arrays.

Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download
10008254 Instruction Manual, ProteinChip Q Spin Columns, Rev D Click to download
10008257 Instruction Manual, ProteinChip IMAC Spin Columns, Rev C Click to download
10008256 Instruction Manual, ProteinChip CM Spin Columns, Rev C Click to download
10008255 Instruction Manual, ProteinChip Q Filtration Plate, Rev D Click to download
10008251 Instruction Manual, ProteinChip Affinity Beads, Rev C Click to download