UNOsphere SUPrA Media

Print
UNOsphere SUPrA Media

Overview

Affinity Media Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi (3.1 bar) Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, provided charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi (6.8 bar) Purification of recombinant His-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi (3.1 bar) Purification of recombinant GST-tagged proteins
Profinity eXact*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free protein by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi (1 bar) Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG, per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi (1 bar) Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum protiens 0.14 ml serum/ml gel 2–10 15 psi (1 bar) Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum protiens 0.17–0.5 ml serum/ml gel 2–11 15 psi (1 bar) Produces albumin and protease-free antibody preparation from serum without prior dialysis
Affi-Prep polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi (70 bar) Endotoxin removal
Affi-Gel boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi (1 bar) Adsorption of cis-hydroxyl-containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi (5.5 bar) Activated matrix for the immobilization of barious ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi (1 bar) For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi (1 bar) For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi (1 bar) Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi (1 bar) Carbodimide coupling of carboxyl-containing ligand

* Refer to bulletin 3193 for purification conditions.
** For example, Profinity GST resin is only available in prepacked cartridges.
***Profinity eXact purification resin.

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2-1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
Histidine-tagged protein
780-0811 780-0812
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Monoclonal Antibody Purification Workflow
Monoclonal Antibody Purification Workflow

Workflow for mAb purification with UNOsphere SUPra affinity, UNOsphere Q and CHT type I media.

PDF Bulletin 5728 To read more click here

Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material of animal origin.

The UNOsphere base bead is a macroporous polymeric bead that is designed for robust and scalable process applications. The outstanding flow-pressure performance of UNOsphere chromatography media allows their use in large-scale process applications.

UNOsphere SUPrA affinity chromatography support comes with full regulatory support and is backed by the support of Bio-Rad’s global application and development team.

If you are interested in ordering large volume quantities, please contact your local Bio-Rad representative to discuss your requirements.

Composition Highly cross-linked polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Working pH range 3–11
Dynamic Binding Capacity* 30 ± 3 mg/ml at 150 cm/hr
25 ± 2 mg/ml at 300 cm/hr
20 ± 2 mg/ml 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical Stability 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-In-Place (CIP) Solutions Cleaning-In-Place (CIP) Solutions 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range 100–600 cm/hr
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C
*  10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
**No significant change in chromatographic performance
 
Foresight™ UNOsphere SUPrA™ Plates, 20 µl
Foresight™ UNOsphere SUPrA™ Plates, 20 µl

732-4709
2 x 96-well plates, 20 µl, filter plate prepacked with UNOsphere SUPrA affinity chromatography resin

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Foresight™ UNOsphere SUPrA™ Column, 1 ml
Foresight™ UNOsphere SUPrA™ Column, 1 ml

732-4729
Pkg of 1, 1 ml, column prepacked with UNOsphere SUPrA affinity chromatography resin

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Foresight™ UNOsphere SUPrA™ Column, 5 ml
Foresight™ UNOsphere SUPrA™ Column, 5 ml

732-4749
Pkg of 1, 5 ml, column prepacked with UNOsphere SUPrA affinity chromatography resin

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Foresight™ UNOsphere SUPrA™ RoboColumn Unit, 200 µl
Foresight™ UNOsphere SUPrA™ RoboColumn Unit, 200 µl

732-4834
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with UNOsphere SUPrA affinity chromatography resin

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Foresight™ UNOsphere SUPrA™ RoboColumn Unit, 600 µl
Foresight™ UNOsphere SUPrA™ RoboColumn Unit, 600 µl

732-4835
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with UNOsphere SUPrA affinity chromatography resin

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
UNOsphere SUPrA Affinity Chromatography Media

156-0218
25 ml, protein A affinity chromatography media

List Price:   $600.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
UNOsphere SUPrA Affinity Chromatography Media

156-0219
100 ml, protein A affinity chromatography media

List Price:   $1,400.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
UNOsphere SUPrA Affinity Chromatography Media

156-0220
500 ml, protein A affinity chromatography media

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
UNOsphere SUPrA Affinity Chromatography Media

156-0221
5 L, protein A affinity chromatography media

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
UNOsphere SUPrA Affinity Chromatography Media

156-0222
10 L, protein A affinity chromatography media

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Number Description Options
6053 Application of UNOsphere SUPrA Media, a New Protein A Affinity Chromatpgraphic Support, for Both Laboratory-Scale and Industrial Antibody Purification, Rev A Click to download
5729 UNOsphere SUPrA Affinity Chromatography Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5728 Monoclonal Antibody Purification Using UNOsphere SUPrA Media, Rev A Click to download [ Add to Cart (Free) ]
10014997 Instruction Manual, Bio-Scale Mini UNOsphere SUPrA cartridges, 1 and 5 ml, Rev A Click to download
10014430 Instruction Manual, UNOsphere SUPrA Affinity Chromatography Media, Rev A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download