Protein G Magnetic Beads

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Protein G Magnetic Beads

Overview

SureBeads™ Immunoprecipitation Protocol

Standard immunoprecipitation protocol using the SureBeads System.

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SureBeads™ Magnetic Bead System for Immunoprecipitation
Performance of SureBeads Magnetic Beads vs. Agarose Beads

SureBeads Protein A Magnetic Beads pull down approximately four times more NAD(P)H dehydrogenase [quinone] 1 (NQO1) protein from HeLa cell lysates than do equal amounts of protein A agarose beads, improving yields and reducing antibody consumption.

Comparison of immunoprecipitation (IP) performance of SureBeads magnetic beads and agarose beads

Comparison of the immunoprecipitation (IP) performance of SureBeads Magnetic Beads and agarose beads.

Read the complete testing protocol in Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads (Bulletin 6594).

Download PDF: Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads, Rev A (Bulletin 6594)
Antibody Binding Affinities of Protein A and Protein G
Monoclonal Protein A Protein G
Human IgG1 +++ +++
Human IgG2 +++ +++
Human IgG3 +++
Human IgG4 +++ +++
Mouse IgG1 + +++
Mouse IgG2a +++ +++
Mouse IgG2b ++ ++
Mouse IgG3 ++ ++
Rat IgG1 +
Rat IgG2aa +++
Rat IgG2b ++
Rat IgG2c + ++
Polyclonal Protein A Protein G
Rabbit +++ ++
Cow ++ +++
Horse ++ +++
Goat ++
Guinea pig +++ ++
Sheep +/- ++
Pig ++ ++
Rat +/- ++
Mouse ++ ++
Chicken +
Human IgG +++ +++
Human IgM +
Human IgD +
Human IgA +

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Binding affinities of protein A and G to various species and subclasses of monoclonal and polyclonal antibodies.

SureBeads™ Protein G Magnetic Beads and Magnetic Racks are designed for quick and easy immunoprecipitation (IP) and protein complex pull-down. SureBeads Magnetic Beads contain a superparamagnetic core, which enables rapid and highly efficient immunoprecipitation without the need for centrifugation.

Immunoprecipitation in five easy steps:

Immunoprecipitation in five easy steps

Download the SureBeads protocol for more information on immunoprecipitation with the SureBeads System.

Trial Size Available
Try a SureBeads Trial Pack.
Includes 1 ml conjugated magnetic beads and a mini magnetic rack for 4 microcentrifuge tubes.
SureBeads trial pack

Features and Benefits of SureBeads Protein G Magnetic Beads

  • Faster IP — magnetization is faster than centrifugation
  • Easier IP — beads cling magnetically to the side wall of the tube, not the bottom, for easier pipetting
  • Reliable IP — consistent IgG binding capacity ensures accurate, reproducible results. Less nonspecific binding than agarose or Sepharose Beads
  • Save on antibody costs — unique surface chemistry enables you to use less antibody
  • Easy to use — ergonomically designed SureBeads Rack with separable magnets works in seconds to magnetize the beads

Applications and Uses of SureBeads Protein G Magnetic Beads

  • Immunoprecipitation for protein isolation
  • Co-immunoprecipitation (Co-IP) or protein complex pull-down
  • High-affinity binding of human, rat, and mouse IgGs

Packaging Options

Conjugated Protein Bead Suspension Volume Rack Size
(Number of Tubes)
Catalog #
G 1 ml 161-4021
G 3 ml 161-4023
G 1 ml 4* 161-4821
A 1 ml 161-4011
A 3 ml 161-4013
A 1 ml 4* 161-4811
A/G 2 x 3 ml 16 161-4833
G/G 2 x 3 ml 16 161-4823
A/A 2 x 3 ml 16 161-4813
16-tube magnetic rack  
16 161-4916

*Trial kit

More Information

Protein A/G Immunoprecipitation Guide

Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.

Monoclonal Protein A Protein G
Human IgG1 +++ +++
Human IgG2 +++ +++
Human IgG3 +++
Human IgG4 +++ +++
Mouse IgG1 + +++
Mouse IgG2a +++ +++
Mouse IgG2b ++ ++
Mouse IgG3 ++ ++
Rat IgG1 +
Rat IgG2aa +++
Rat IgG2b ++
Rat IgG2c + ++
Polyclonal Protein A Protein G
Rabbit +++ ++
Cow ++ +++
Horse ++ +++
Goat ++
Guinea pig +++ ++
Sheep +/- ++
Pig ++ ++
Rat +/- ++
Mouse ++ ++
Chicken +
Human IgG +++ +++
Human IgM +
Human IgD +
Human IgA +

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Related Products

Bead size 2.4–3.4 µm
Bead magnetic content 14–28%
Typical IgG binding capacity ≥6 µg/mg
Solid content in slurry 1.0–1.2%
Protein A/G content 9–12 µg/mg
Dispersion medium TBS + 0.05% Tween 20 + 0.09% sodium azide
SureBeads™ Trial Kit Protein G

161-4821
Trial kit for protein G conjugated magnetic beads for immunoprecipitation, contains 1 ml bead suspension (10 mg beads/ml suspension) and a 4-tube magnetic rack

List Price:   $70.00
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SureBeads™ Protein G Magnetic Beads, 3 ml

161-4023
3 ml, suspension of protein G conjugated magnetic immunoprecipitation beads (10 mg beads/ml)

List Price:   $150.00
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SureBeads™ Starter Kit Protein A and G

161-4833
Starter kit for immunoprecipitation with protein A and G conjugated magnetic beads; 3 ml protein G bead suspension, 3 ml protein A bead suspension (10 mg beads/ml suspension), and 16-tube magnetic rack

List Price:   $550.00
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SureBeads™ Starter Kit Protein G

161-4823
Starter kit for immunoprecipitation with protein G conjugated magnetic beads, contains 2 x 3 ml protein G bead suspension (10 mg beads/ml suspension) and a 16-tube magnetic rack

List Price:   $550.00
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SureBeads™ Protein G Magnetic Beads, 1 ml

161-4021
1 ml, suspension of protein G conjugated magnetic immunoprecipitation beads (10 mg beads/ml)

List Price:   $80.00
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16-Tube SureBeads™ Magnetic Rack

161-4916
Rack with high-strength magnets for rapid magnetic bead separation. Supports 16 x 1.5 ml microcentrifuge tubes or 2 x 15 ml conical tubes. Use with SureBeads Magnetic Beads.

List Price:   $325.00
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Number Description Options
6560 SureBeads™ Magnetic Beads Competitive Sales Flyer, Rev B Click to download [ Add to Cart (Free) ]
6594 Immunoprecipitation Performance of SureBeads™ Protein A Magnetic Beads vs. Protein A Agarose Beads, Rev A Click to download
10041689 SureBeads™ Magnetic Beads for Immunoprecipitation Product Insert, Rev A Click to download
10043651 SureBeads™ Magnetic Beads Standard Immunoprecipitation Protocol, Rev A Click to download