PROTEAN II xi Cell

Print

Overview

Specifications
  PROTEAN II® xi (16 cm) PROTEAN II xi (20 cm) PROTEAN II XL (20 cm)
Number of gels 1–4 1–4 1–4
Gel size (W x L) 16 x 16 cm (handcast) 16 x 20 cm (handcast) 18.3 x 20 cm (handcast)
18.3 x 19.3 cm (precast)
Glass plate size (W x L)      
              Inner plate 20 x 16 cm 20 x 20 cm 20 x 20 cm
              Outer plate 20 x 18.3 cm 20 x 22.3 cm 20 x 22.3 cm
Spacer length 18.3 cm 22.3 cm 22.3 cm
Typical upper buffer volume 350 ml 350 ml 350 ml
Typical lower buffer volume 1.8 L 1.2 L 1.2 L
Typical run times for SDS-PAGE      
              Without cooling 4 hr 5 hr 5 hr
              With cooling 2.5 hr 3.5 hr 3.5 hr
Recommended power supply PowerPac™ HV or PowerPac Universal PowerPac™ HV or PowerPac Universal PowerPac™ HV or PowerPac Universal
Component Comparison

PROTEAN® II xi and XL (IPG) component comparison

  1. The 4 mm xi and 13 mm XL clamp notch
  2. The 19 mm xi and 8 mm XL spacer
  3. The 181 mm xi and 198 mm XL central cooling core gasket
  4. The 153 mm xi and 184 mm XL comb
Sample Volume per Well for PROTEAN® II xi and XL Combs*
 
Comb Thickness
Number or Type of Wells Well Width 0.5 mm 0.75 mm 1.0 mm 1.5 mm 3.0 mm
25
3.5 mm

60 µl
80 µl
120 µl
20
5.0 mm
54 µl
82 µl
110 µl
164 µl
328 µl
15
6.5 mm
74 µl
110 µl
147 µl
221 µl
442 µl
10
1.0 cm
114 µl
172 µl
229 µl
343 µl
687 µl
5
2.3 cm
522 µl
783 µl
1.57 ml
3
4.0 cm
1.37 ml
Blank
14.5 cm
2.44 ml
3.26 ml
4.88 ml
9.76 ml
2-D Comb
  Reference well
3.5 mm
28 µl
42 µl
84 µl
  Sample well
17.8–17.9 cm
* At standard 25 mm well depth.

The PROTEAN® II xi cell is a large format vertical electrophoresis cell that is used for the most common electrophoretic techniques such as SDS-PAGE, native electrophoresis, and agarose gel electrophoresis. The PROTEAN II xi cell is available in two sizes to accommodate up to four* 16 x 16 cm or 16 x 20 cm gels.

Key Features

  • Accommodates handcast gels
  • Patented single-screw clamps on the casting stand that exert uniform pressure along the entire length of the glass plates provide a leakproof seal without agarose plugs or grease
  • Central cooling core can be connected to tap water or a cooled recirculating bath, or filled with coolant, providing smile-free patterns with as little as 1.5 L buffer
  • Choice of glass plates, spacers, and sandwich clamps to cast 2 gel lengths, 16 or 20 cm
  • Ability to cast multiple gradient gels using the PROTEAN II multi-gel casting chamber, the Model 495 gradient former, and the appropriate exponential piston. Use of the gel clip facilitates handling of large gels
  • Notched plates allow up to four 16 x 16 cm or 16 x 20 cm handcast gels to be run simultaneously (not recommended for 2-D applications)
  • Optional frosted glass plates for high-resolution vertical agarose gel electrophoresis of nucleic acids
  • Comb conversion screws convert any PROTEAN II xi comb from a standard 25 mm well depth to a 10 mm depth to simplify comb removal

PROTEAN II IPG Conversion Kits for 2-D Applications

Conversion kits expand the capabilities of the PROTEAN II xi cell to include applications such as tube gel IEF and second-dimension SDS-PAGE. To convert the PROTEAN II xi cell into a:

  • PROTEAN xi 2-D cell — use available adaptors and other accessories to accommodate 16 tube gels for IEF applications
  • PROTEAN II XL cell — use the PROTEAN II IPG conversion kit for second-dimension electrophoresis of 17 cm IPG strips

Each cell includes the central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, 4 sandwich clamps, upper buffer dam, casting stand with gaskets, leveling bubble, and instructions. The 16 cm cells include clamps for running 16 cm gels; the 20 cm cells include clamps for 20 cm gels. Cells without combs and spacers (catalog #165-1801 and #165-1811) allow you to select the spacer thickness and comb that best fit your application. See the Accessories tab for a list of available combs and spacers.

* For higher throughput, the PROTEAN II xi multi-cell provides 6-gel capacity for second-dimension runs.

PROTEAN II xi Cell

165-1801
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble; spacers and combs not included

List Price:   $1,517.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1802
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 1.5 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price:   $1,563.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1803
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 1.0 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price:   $1,609.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1804
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 0.75 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price:   $1,563.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1811
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, spacers and combs not included

List Price:   $1,578.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1812
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 1.5 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price:   $1,573.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1813
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 1.0 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price:   $1,619.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell

165-1814
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 0.75 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price:   $1,573.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Cell 2-D Conversion Kit

165-1815
Kit converts PROTEAN II xi cell into a tube gel IEF 2-D system; includes 2 tube gel adaptors, 24 glass tubes (1.5 mm ID, 180 mm length), gaskets, grommets, stoppers

List Price:   $661.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PROTEAN II xi Basic Unit With Casting Stand

165-1834
Vertical electrophoresis system, includes electrophoresis cell with central cooling core, gel casting stand

List Price:   $1,332.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
4106107 Instruction Manual, PROTEAN II xi Cell IPG Conversion Kit, Rev B Click to download
M1651801 Instruction Manual, PROTEAN II xi Cell, Rev C Click to download [ Add to Cart (Free) ]
1760 PROTEAN II xi and XL Cells PIS, Rev F Click to download [ Add to Cart (Free) ]
3095 2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B Click to download [ Add to Cart (Free) ]