Profinity eXact™ Purification and Tag Cleavage Consumables

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Profinity eXact Purification and Fusion-Tag Cleavage Consumables

Overview

Profinity eXact™ Fusion-Tagged Protein Purification
SDS-PAGE analysis of a purification of Profinity eXact fusions to a 26 kD Green Fluorescent Protein (GFP) and to a 40 kD maltose binding protein (MBP).

SDS-PAGE analysis of a purification of Profinity eXact fusions to a 26 kD green fluorescent protein (GFP) and to a 40 kD maltose binding protein (MBP). Crude E. coli lysate containing the expressed proteins (2 ml) was loaded onto a Bio-Scale Mini Profinity eXact 1 ml cartridge on a BioLogic DuoFlow™ system with bind/wash buffer at 1 ml/min. The cartridge was washed with 10 column volumes (CV) of the same buffer at 1 ml/min. Proteins were eluted with 3 CV sodium fluoride buffer at room temperature and at 0.1 ml/min for 30 min. Total purification time required to generate the tag-free proteins without addition of protease was approximately 60 min.

Stability and Reusability of Profinity eXact™ Purification Resin
Overlaid chromatograms of MBP purification

Stability and reusability of Profinity eXact purification resin. Overlaid chromatograms of MBP purification with a 1 ml Bio-Scale Mini Profinity eXact cartridge on the BioLogic DuoFlow system. Binding capacities for cycles 1 to 5 ranged from 3.7 mg/ml to 3.3 mg/ml.

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Profinity eXact consumables include Profinity eXact purification resin, Profinity eXact mini spin columns, Bio-Scale Mini Profinity eXact cartridges, and the Profinity eXact expression and purification starter kit.

Profinity eXact Purification Resin

The Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. Purity of the eluted protein using this purification resin is typically higher than that for other affinity-tag systems. This is due to the specific recognition of subtilisin for its prodomain sequence. The resin can be packed into different column formats, including low- to medium-pressure columns, gravity-flow columns, and mini spin columns, offering users added purification flexibility.

Additional advantages of Profinity eXact purification resin include:

  • Purification and processing of fusion-tagged proteins in a single-step
  • On-column cleavage in as little as 30 min
  • No protease addition required
  • Precise cleavage at N-terminus to generate native protein sequence

The resin is supplied in 100 mM sodium phosphate, pH 7.2, containing 0.02% sodium azide. It is also available prepacked in 1 ml and 5 ml cartridges, and in mini spin columns.

Profinity eXact Mini Spin Columns

Profinity eXact mini spin columns are ideal for rapid expression screening and optimization of affinity-tag purification protocols. These single-use columns contain 0.1 ml settled Profinity eXact resin and all necessary plasticware for binding, washing, and elution steps. Profinity eXact mini spin columns have been designed to fit in all major benchtop centrifuges. The mini spin columns are available separately or in kits:

  • Profinity eXact mini spin column ten-packs (each column contains 0.1 ml settled resin) with collection tubes for binding, washing, and elution steps
  • Profinity eXact mini spin purification starter kit (includes ten mini spin columns, collection tubes, control protein lysate, bacterial lysis reagent, bind/wash buffer, and elution buffer)
  • Profinity eXact expression and purification starter kit (includes Profinity eXact mini spin purification starter kit and Profinity eXact cloning and expression starter kit)

Bio-Scale Mini Profinity eXact Cartridges

These 1 ml and 5 ml cartridges can be used with BioLogic chromatography systems, the Profinia protein purification system, a peristaltic pump, or with appropriate column adaptors with any other chromatography system. These cartridges are prepacked with Profinity eXact purification resin.

Profinity eXact Expression and Purification Starter Kit

The Profinity eXact expression and purification starter kit can be used to easily evaluate Profinity eXact fusion-tag technology and is suitable for new purifications requiring tag removal and for purifications where cleavage has resulted in inferior results. The Profinity eXact expression and purification starter kit includes two kits: the Profinity eXact cloning and expression starter kit for the cloning and expression of a target gene using pPAL vectors and competent cells, and the Profinity eXact mini spin purification starter kit for single-step purification and on-column cleavage of the tagged protein.

Functional ligand Subtillisin protease, 27.8 kD
Base bead 6% agarose bead
Form 50% suspension in 100 mM sodium phosphate, pH 7.2, 0.02% sodium azide
Practical size range 60–160 µm
Dynamic binding capacity >3 mg tag-free protein/ml resin
Recomended linear flow rate 1,000 cm/hr at 25°C
pH stability 2–13
Chemical compatibility Common reagents including detergents, reducing agents, buffering agents, and additives.
Storage 4°C
Shelf life in 20% ethanol >1year at 4°C
Operational Temperature 4–40°C
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Profinity eXact™ Purification Resin, 10 ml

1563005
10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide

0 1563005 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3005_0921120636306.xml 1563005 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0terhjao0teri" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0terhjao0teri"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3005_0921120636306.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0tepk 2017-11-14 07:07:54 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:16:16 1563005-profinity-exact-purification-resin-10-ml 1563005 Profinity eXact Purification Resin Profinity eXact™ Purification Resin, 10 ml 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-3005_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-3005_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-3005_feature.jpg preview RepEx Profinity eXact Purification Resin 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide 1563005, profinity, exact, purification, resin, affinity, tagged, affinity-tagged, chromatography, subtilisin, protease, conjugated, agarose-based, matrix 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 <p>Choose Profinity eXact Purification Resin to simplify purification and tag removal of fusion-tagged proteins. This resin can be used with a range of reagents, including detergents, reducing agents, buffering agents, and a variety of additives.</p> <ul> <li>Consists of a Superflow agarose bead, which uses the modified subtilisin protease as its ligand</li> <li>Fusion-tag proteins contain prodomain sequence of ligand</li> <li>Provides high mechanical stability to withstand the pressure limits of low-to-medium column chromatography</li> <li>Stable across a wide pH range (2&ndash;13)</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>Profinity eXact Purification Resin is also available in <a href="/en-us/product/bio-scale-mini-profinity-exact-cartridges">prepacked cartridges</a> and <a href="/en-us/sku/1563007-profinity-exact-mini-spin-columns">spin columns</a>.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a>, hold up to 2 ml support</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a>, hold up to 20 ml support</li> <li><a href="/en-us/product/empty-spin-columns?ID=612dbf40-5acd-468f-8eef-b855ce941865">Spin Columns</a></li> <li><a href="/en-us/product/profinia-desalting-kits">Profinia Desalting Kits</a></li> </ul> <h2 class="descHeader">Related Cateogories</h2> <ul> <li>Profinity eXact&trade; Fusion-Tag System</li> <li><a href="/en-us/product/profinity-exact-expression-vector-kits-cloning-products">Profinity eXact Expression Vector Kits and Cloning Products</a></li> <li><a href="/en-us/product/profinity-exact-purification-tag-cleavage-consumables">Profinity eXact&trade; Purification and Tag Cleavage Consumables</a></li> </ul> <h2 class="descHeader">Related Content</h2> <ul> <li><a href="/en-us/category/activated-affinity-media">Activated Affinity Media</a></li> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Quantitation</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <p>Profinity eXact Purification Resin <a href="/webroot/web/pdf/lsr/literature/Bulletin_5655.pdf" target="_blank">Information Sheet</a></p> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-exact-purification-resin-10-ml-1563005.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-exact-purification-resin-10-ml-1563005.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563005&CartQuote=QT&quantity= false true false $604.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563005&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563005&quantity= 1563005
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Profinity eXact™ Mini Spin Columns

1563007
Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes

1 1563007 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml 1563007 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teph 2017-11-14 07:07:54 true true internet/product_SKU jao0teph templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml LSR 2018-03-06 23:49:41 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 23:49:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml 1563007-profinity-exact-mini-spin-columns 1563007 Profinity eXact Mini Spin Columns Profinity eXact™ Mini Spin Columns Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes Pkg of 10 mini spin columns packed with 0.1 ml Profinity eXact resin, includes 50 capped, collection tubes Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-3007_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-3007_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-3007_feature.jpg preview RepEx Profinity eXact Mini Spin Columns Pkg of 10 mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes 1563007, profinity, exact, mini, spin, columns, purification, resin, affinity, tagged, affinity-tagged, chromatography, subtilisin, protease, conjugated, agarose-based, matrix 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 <p>Choose Profinity eXact Mini Spin Columns for rapid purification and in-line tag removal of Profinity eXact-tagged proteins by affinity chromatography. The spin columns can be used with a standard centrifuge and one purification run takes less than one hour.</p> <ul> <li>Consists of a novel affinity medium immobilized with a modified version of the subtilisin protease</li> <li>Allows the highly specific protease to bind strongly to the Profinity eXact tag with sub-nanomolar affinity</li> <li>Helps cleave and elute the tag-free target protein from the column with its native or desired N-terminal amino acids</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>Profinity eXact Purification Resin (<a href="/en-us/sku/1563005-profinity-exact-purification-resin-10-ml">1563005</a>)</li> <li>Profinity eXact Mini Purification Starter Kit (<a href="/en-us/sku/1563006-profinity-exact-mini-spin-purification-starter-kit">1563006</a>)</li> <li>Bio-Scale Mini-Profinity eXact Cartridges, 1 ml (<a href="/en-us/sku/7324646-bio-scale-mini-profinity-exact-cartridges">7324646</a>)</li> <li>Bio-Scale Mini-Profinity eXact Cartridge, 5 ml (<a href="/en-us/sku/7324648-bio-scale-mini-profinity-exact-cartridge">7324648</a>)</li> </ul> <h2 class="descHeader">Related Cateogories</h2> <ul> <li><a href="/en-us/category/profinity-exact-fusion-tag-system">Profinity eXact Fusion-Tag System</a></li> <li> <a href="/en-us/product/profinity-exact-expression-vector-kits-cloning-products">Profinity eXact Expression Vector Kits and Cloning Products</a></li> <li> <a href="/en-us/product/profinity-exact-purification-tag-cleavage-consumables">Profinity eXact Purification and Tag Cleavage Consumables</a> <ul> </ul> </li> </ul> <h2 class="descHeader">Related Content</h2> <ul> <li><a href="/en-us/category/activated-affinity-media">Activated Affinity Media</a></li> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Quantitation</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <p>Profinity eXact Purification Resin <a href="/webroot/web/pdf/lsr/literature/Bulletin_5655.pdf" target="_blank">Information Sheet</a></p> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs/autopopulated 156-3007.html /webroot/web/html/lsr/products/SKUs/autopopulated/156-3007.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563007&CartQuote=QT&quantity= false true false $175.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563007&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563007&quantity= 1563007
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Bio-Scale Mini Profinity eXact Cartridges

7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

2 7324646 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4646_0921120619125.xml 7324646 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcv374gwjcv374gx" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv374gwjcv374gx"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4646_0921120619125.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Node> <Node Name="PROD"> <Deployment Id="StandardMetadata.jcv375apjcv375aq" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv375apjcv375aq"> <Name>StandardMetadata</Name> <TargetEnviroment>PROD</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4646_0921120619125.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0tepq 2017-11-14 07:08:06 true true internet/product_SKU LSR N en 09/21/19 12:06 PM 1517016309408 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:06 7324646-bio-scale-mini-profinity-exact-cartridges 7324646 Bio-Scale Mini Profinity eXact Cartridges Bio-Scale Mini Profinity eXact Cartridges Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4646_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4646_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4646_feature.jpg RepEx 732-4646 Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324646&CartQuote=QT&quantity= false true false $236.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324646&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324646&quantity= 7324646
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Bio-Scale Mini Profinity eXact Cartridges

7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

3 7324647 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4647_0921120619483.xml 7324647 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcv374gwjcv374gx" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv374gwjcv374gx"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4647_0921120619483.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Node> <Node Name="PROD"> <Deployment Id="StandardMetadata.jcv375apjcv375aq" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv375apjcv375aq"> <Name>StandardMetadata</Name> <TargetEnviroment>PROD</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4647_0921120619483.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teqt 2017-11-14 07:08:06 true true internet/product_SKU LSR N en 09/21/19 12:06 PM 1517016309450 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:06 7324647-bio-scale-mini-profinity-exact-cartridges 7324647 Bio-Scale Mini Profinity eXact Cartridges Bio-Scale Mini Profinity eXact Cartridges Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4647_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4647_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4647_feature.jpg RepEx 732-4647 Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324647&CartQuote=QT&quantity= false true false $404.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324647&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324647&quantity= 7324647
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Bio-Scale Mini Profinity eXact Cartridge

7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

4 7324648 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4648_0921120619756.xml 7324648 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcv374gwjcv374gx" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv374gwjcv374gx"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4648_0921120619756.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Node> <Node Name="PROD"> <Deployment Id="StandardMetadata.jcv375apjcv375aq" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv375apjcv375aq"> <Name>StandardMetadata</Name> <TargetEnviroment>PROD</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4648_0921120619756.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0terb 2017-11-14 07:08:06 true true internet/product_SKU LSR N en 09/21/19 12:06 PM 1517016309586 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:06 7324648-bio-scale-mini-profinity-exact-cartridge 7324648 Bio-Scale Mini Profinity eXact Cartridge Bio-Scale Mini Profinity eXact Cartridge Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4648_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4648_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4648_feature.jpg RepEx 732-4648 Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324648&CartQuote=QT&quantity= false true false $436.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324648&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324648&quantity= 7324648
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(Discontinued) Profinity eXact Expression and Purification Starter Kit

1563008
Kit includes 1 Profinity eXact cloning and expression starter kit (20 reactions) and 1 Profinity eXact mini spin purification starter kit (10 spin columns)

This product is no longer available. Please contact technical support for more information on replacement product.
Profinity eXact Mini Spin Purification Starter Kit

1563006
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers

6 1563006 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3006_0921120636233.xml 1563006 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0terhjao0teri" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0terhjao0teri"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3006_0921120636233.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teqd 2017-11-14 07:07:54 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:54 1563006-profinity-exact-mini-spin-purification-starter-kit 1563006 Profinity eXact Mini Spin Purification Starter Kit Profinity eXact Mini Spin Purification Starter Kit Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-3008_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-3008_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-3008_feature.jpg RepEx 156-3006 Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 / false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563006&CartQuote=QT&quantity= false true false $515.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563006&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563006&quantity= 1563006
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Number Description Options
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6334 Tips and Tricks for Successful Preparative Chromatography and Protein Purification, Rev B Click to download
5652 Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A Click to download
5656 Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B Click to download
5668 Profinity eXact Fusion-Tag System Brochure, Rev A Click to download [ Add to Cart (Free) ]
5646 Profinity eXact™ Cloning and Expression Kits, PIS, Rev B Click to download
5655 Profinity eXact™ Resin Product Information Sheet, Rev A Click to download
5811 Profinity eXact Fusion-Tag System FAQs, Rev A Click to download
5898 Using the Profinity eXact Fusion-Tag System: Strategies for Success, Rev A Click to download
10011161 BL21(DE3) Chemi-Competent Expression Cells Manual, Rev A Click to download
10011164 Bio-Scale Mini Profinity eXact™ Cartridges Manual, Rev B Click to download
10011260 Profinity eXact Protein Purification System Manual, Rev A Click to download
5772 Periplasmic Expression and Purification of Recombinant Proteins from E.coli Using the Profinity eXact Fusion-Tag System, Rev A Click to download [ Add to Cart (Free) ]
5928 Application of the Profinity eXact Fusion-Tag System to Eukaryotic Expression Systems Tech Note, Rev B Click to download

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