Profinity Exact™ Purification Resin

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Profinity Exact™ Purification Resin

Overview

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

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Profinity eXact Purification Resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The resulting purity of the eluted protein is typically higher than that for other affinity-tag systems due to the specific recognition (KD < 100 pM) that subtilisin has for its prodomain sequence (the Profinity eXact Tag). Once the Profinity eXact fusion protein has been captured by the Profinity eXact Purification Resin — and the cleavage reaction has been triggered with a sodium fluoride-containing buffer — the affinity tag is precisely cleaved at the C-terminus of the cleavage recognition sequence (EEDKLFKAL). The result is a quickly purified protein product that lacks any amino acid residues derived from the Profinity eXact Tag.

Features and Benefits of Profinity eXact Resin

  • Purification and processing of fusion-tagged proteins in a single-step
  • On-column cleavage in as little as 30 min
  • No protease addition required
  • Precise cleavage at N-terminus to generate native protein sequence

The following convenient packaging configurations allow easy evaluation of Profinity eXact technology:

  • Profinity eXact Resin — 10 ml affinity resin supplied in 100 mM sodium phosphate, pH 7.2 containing 0.02% sodium azide
  • Profinity eXact Mini Spin Columns — 10-pack of single-use columns that contain 0.1 ml settled resin in addition to all required collection tubes for binding, washing, and elution steps. Fit all benchtop centrifuges
  • Profinity eXact Mini Spin Starter Kit — comprehensive 10-pack kit that ensures purification success upon initial trial. Includes 10 prepacked spin columns, collection tubes, lysis reagent, buffers for purification, and lyophilized MBP control protein lysate
  • Bio-Scale Mini Profinity eXact Cartridges — 1 ml and 5 ml cartridges that can be used with BioLogic chromatography systems, the Profinia™ Protein Purification System, a peristaltic pump, or with appropriate column adaptors with any other chromatography system
  • Profinity eXact Expression and Purification Starter Kit — includes two kits:
    • 1 Profinity eXact Cloning and Expression Starter Kit for the cloning and expression of a target gene using pPAL vectors and competent cells
    • 1 Profinity eXact Mini Spin Purification Starter Kit for single-step purification and on-column cleavage of the tagged protein

More Information

Profinity eXact™ Resin Product Information Sheet (Bulletin 5655)

Profinity eXact™ Fusion-Tag System Brochure (Bulletin 5668)

Profinity eXact™ Protein Purification System Manual (Bulletin 10011260)

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Profinity eXact™ Purification Resin, 10 ml

1563005
10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide

2 1563005 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3005_0921120636306.xml 1563005 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0terhjao0teri" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0terhjao0teri"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3005_0921120636306.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0tepk 2017-11-14 07:07:54 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:16:16 1563005-profinity-exact-purification-resin-10-ml 1563005 Profinity eXact Purification Resin Profinity eXact™ Purification Resin, 10 ml 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-3005_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-3005_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-3005_feature.jpg preview RepEx Profinity eXact Purification Resin 10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide 1563005, profinity, exact, purification, resin, affinity, tagged, affinity-tagged, chromatography, subtilisin, protease, conjugated, agarose-based, matrix 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 <p>Choose Profinity eXact Purification Resin to simplify purification and tag removal of fusion-tagged proteins. This resin can be used with a range of reagents, including detergents, reducing agents, buffering agents, and a variety of additives.</p> <ul> <li>Consists of a Superflow agarose bead, which uses the modified subtilisin protease as its ligand</li> <li>Fusion-tag proteins contain prodomain sequence of ligand</li> <li>Provides high mechanical stability to withstand the pressure limits of low-to-medium column chromatography</li> <li>Stable across a wide pH range (2&ndash;13)</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>Profinity eXact Purification Resin is also available in <a href="/en-us/product/bio-scale-mini-profinity-exact-cartridges">prepacked cartridges</a> and <a href="/en-us/sku/1563007-profinity-exact-mini-spin-columns">spin columns</a>.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a>, hold up to 2 ml support</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a>, hold up to 20 ml support</li> <li><a href="/en-us/product/empty-spin-columns?ID=612dbf40-5acd-468f-8eef-b855ce941865">Spin Columns</a></li> <li><a href="/en-us/product/profinia-desalting-kits">Profinia Desalting Kits</a></li> </ul> <h2 class="descHeader">Related Cateogories</h2> <ul> <li>Profinity eXact&trade; Fusion-Tag System</li> <li><a href="/en-us/product/profinity-exact-expression-vector-kits-cloning-products">Profinity eXact Expression Vector Kits and Cloning Products</a></li> <li><a href="/en-us/product/profinity-exact-purification-tag-cleavage-consumables">Profinity eXact&trade; Purification and Tag Cleavage Consumables</a></li> </ul> <h2 class="descHeader">Related Content</h2> <ul> <li><a href="/en-us/category/activated-affinity-media">Activated Affinity Media</a></li> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Quantitation</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <p>Profinity eXact Purification Resin <a href="/webroot/web/pdf/lsr/literature/Bulletin_5655.pdf" target="_blank">Information Sheet</a></p> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-exact-purification-resin-10-ml-1563005.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-exact-purification-resin-10-ml-1563005.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563005&CartQuote=QT&quantity= 1332 false true false $604.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563005&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563005&quantity= 1563005
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(Discontinued) Profinity eXact Expression and Purification Starter Kit

1563008
Kit includes 1 Profinity eXact cloning and expression starter kit (20 reactions) and 1 Profinity eXact mini spin purification starter kit (10 spin columns)

This product is no longer available. Please contact technical support for more information on replacement product.
Profinity eXact Mini Spin Purification Starter Kit

1563006
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers

4 1563006 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3006_0921120636233.xml 1563006 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0terhjao0teri" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0terhjao0teri"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3006_0921120636233.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teqd 2017-11-14 07:07:54 true true internet/product_SKU LSR N en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:54 1563006-profinity-exact-mini-spin-purification-starter-kit 1563006 Profinity eXact Mini Spin Purification Starter Kit Profinity eXact Mini Spin Purification Starter Kit Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-3008_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-3008_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-3008_feature.jpg RepEx 156-3006 Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 / false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563006&CartQuote=QT&quantity= 1332 false true false $515.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563006&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563006&quantity= 1563006
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Profinity eXact pPAL RIC-Ready Expression Vector Kit

1563001
Includes 25 μl of 20 ng/μl pPAL7 restriction-independent cloning (RIC)-ready expression vector; sufficient for 20 reactions

This product is not available for sale online. Please contact customer service for more information.
Opti-4CN Goat Anti-Mouse Detection Kit

1708237
Colorimetric detection (µg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)

5 1708237 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-8237_0921120401196.xml 1708237 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0terhjao0teri" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0terhjao0teri"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-8237_0921120401196.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:04 PM en_US jao0terc 2017-11-14 07:08:34 true true internet/product_SKU LSR N en 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:34 1708237-opti-4cn-goat-anti-mouse-detection-kit 1708237 Opti-4CN Goat Anti-Mouse Detection Kit Opti-4CN Goat Anti-Mouse Detection Kit Colorimetric detection (&micro;g) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm<sup>2</sup> of membrane (~50 mini blots) Colorimetric detection (μg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots) Colorimetric detection (&micro;g) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm<sup>2</sup> of membrane (~50 mini blots) /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-8237_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-8237_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-8237_feature.jpg RepEx 170-8237 Colorimetric detection (μg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots) 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 / false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708237&CartQuote=QT&quantity= 1178 true true false $201.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708237&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708237&quantity= 1708237
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Opti-4CN Substrate Kit

1708235
Colorimetric detection (µg) kit, includes optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)

6 1708235 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-8235_0921120401072.xml 1708235 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jao0tepd 2017-11-14 07:08:34 true true internet/product_SKU jao0tepd templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-8235_0921120401072.xml LSR 2018-06-13 01:14:34 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:04 PM 1517006400151 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-06-13 01:14:34 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-8235_0921120401072.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-8235_0921120401072.xml 1708235-opti-4cn-substrate-kit 1708235 Opti-4CN Substrate Kit Opti-4CN Substrate Kit Colorimetric detection (&micro;g) kit, includes optimized 4CN substrate; for 2,500 cm<sup>2</sup> of membrane (~50 mini blots) Colorimetric detection (μg) kit, includes optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots) Colorimetric detection (&micro;g) kit, includes optimized 4CN substrate; for 2,500 cm<sup>2</sup> of membrane (~50 mini blots) /webroot/web/images/ps/products/chrom_media_sampling/sku_thumb/global/170-8235_tn.jpg /webroot/web/images/ps/products/chrom_media_sampling/sku_view/global/170-8235_view.jpg /webroot/web/images/ps/products/chrom_media_sampling/sku_feature/global/170-8235_feature.jpg RepEx 170-8235 Colorimetric detection (μg) kit, includes optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots) 1708235, opti-4cn, substrate, opti, 4cn, western, blot, blots, blotting, westerns, colorimetric, detection, immun-blot, immunblot, immunodetection, reagents 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 / false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708235&CartQuote=QT&quantity= 1178 true true false $185.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708235&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708235&quantity= 1708235
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Profinity eXact Antibody Reagent

1563004
Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins

7 1563004 /templatedata/internet/product_SKU/data/PSD/Affinity-Media/Profinity-eXact-Purification-R/156-3004_0921120926421.xml 1563004 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jao0terhjao0teri" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jao0terhjao0teri"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/PSD/Affinity-Media/Profinity-eXact-Purification-R/156-3004_0921120926421.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="1" DayOfWeek="5" Year="2017" Hour="19" Minute="16" Second="31" ZoneOffsetMillis="0" Time="1512155791997">Fri Dec 01 19:16:31 UTC 2017</LastExecutionTime> </Node> </Deployments> 09/21/09 12:09 PM en_US jao0ter5 2017-11-14 07:12:16 true true internet/product_SKU PSD N en 09/21/19 12:09 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:12:16 1563004-profinity-exact-antibody-reagent 1563004 Profinity eXact Antibody Reagent Profinity eXact Antibody Reagent Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-3004_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-3004_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-3004_feature.jpg RepEx 156-3004 Pkg of 1 vial, mouse monoclonal antibody, anti subtilisin protease prodomain antibody, recognizes Profinity eXact-tagged fusion proteins 09/21/09 12:09 PM 09/21/19 12:09 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en PSD /PSD/Affinity-Media/Profinity-eXact-Purification-R N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563004&CartQuote=QT&quantity= 1332 false true false $324.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563004&AddToDefaultWishList=true&quantity= true false true 12352200 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563004&quantity= 1563004
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(Discontinued) Profinity eXact Cloning and Expression Starter Kit

1563000
Includes 25 µl of 20 ng/µl pPAL RIC-ready and 100 µl of 100 ng/µl pPAL supercoiled expression vectors, chemi-competent cells, SOC growth media; sufficient for 20 reactions

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) BL21 (DE3) Chemi-Competent Expression Cells

1563003
Pkg of 10 vials, 0.05 ml BL21 (DE3) E. coli cells, also includes pUC 19 control plasmid, 10 ml vial of SOC growth media

This product is no longer available. Please contact technical support for more information on replacement product.
Profinity eXact pPAL Supercoiled Expression Vector Kit

1563002
Includes 100 μl of 100 ng/μl pPAL7 supercoiled plasmid expression vector; sufficient for 20 reactions

10 1563002 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3002_0921120636680.xml 1563002 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jc7v8nysjc7v8nyt" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc7v8nysjc7v8nyt"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="9" DayOfWeek="2" Year="2018" Hour="21" Minute="50" Second="25" ZoneOffsetMillis="0" Time="1515534625653">Tue Jan 09 21:50:25 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3002_0921120636680.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="9" DayOfWeek="2" Year="2018" Hour="21" Minute="50" Second="25" ZoneOffsetMillis="0" Time="1515534625653">Tue Jan 09 21:50:25 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.jc99ds9cjc99ds9d" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99ds9cjc99ds9d"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="10" DayOfWeek="3" Year="2018" Hour="19" Minute="56" Second="18" ZoneOffsetMillis="0" Time="1515614178041">Wed Jan 10 19:56:18 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3002_0921120636680.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="10" DayOfWeek="3" Year="2018" Hour="19" Minute="56" Second="18" ZoneOffsetMillis="0" Time="1515614178041">Wed Jan 10 19:56:18 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.jc99ds9cjc99ds9j" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99ds9cjc99ds9j"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="10" DayOfWeek="3" Year="2018" Hour="20" Minute="1" Second="1" ZoneOffsetMillis="0" Time="1515614461792">Wed Jan 10 20:01:01 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3002_0921120636680.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="10" DayOfWeek="3" Year="2018" Hour="20" Minute="1" Second="1" ZoneOffsetMillis="0" Time="1515614461792">Wed Jan 10 20:01:01 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0tepj 2017-11-14 07:07:54 true true internet/product_SKU LSR N en 09/21/19 12:06 PM 1515615093147 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:54 1563002-profinity-exact-ppal-supercoiled-expression-vector-kit 1563002 Profinity eXact pPAL Supercoiled Expression Vector Kit Profinity eXact pPAL Supercoiled Expression Vector Kit Includes 100 μl of 100 ng/μl pPAL7 supercoiled plasmid expression vector; sufficient for 20 reactions Includes 100 μl of 100 ng/μl pPAL7 supercoiled plasmid expression vector; sufficient for 20 reactions Includes 100 μl of 100 ng/μl pPAL7 supercoiled plasmid expression vector; sufficient for 20 reactions /webroot/web/images/ps/products/chrom_media_sampling/sku_thumb/global/156-3002_tn.jpg /webroot/web/images/ps/products/chrom_media_sampling/sku_view/global/156-3002_view.jpg /webroot/web/images/ps/products/chrom_media_sampling/sku_feature/global/156-3002_feature.jpg RepEx 156-3002 Includes 100 μl of 100 ng/μl pPAL7 supercoiled plasmid expression vector; sufficient for 20 reactions 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563002&CartQuote=QT&quantity= 1332 false true false $232.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563002&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563002&quantity= 1563002
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Profinity eXact™ Mini Spin Columns

1563007
Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes

11 1563007 /templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml 1563007 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0teph 2017-11-14 07:07:54 true true internet/product_SKU jao0teph templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml LSR 2018-03-06 23:49:41 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 23:49:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Affinity-Chromatography-and-Ta/156-3007_0921120636343.xml 1563007-profinity-exact-mini-spin-columns 1563007 Profinity eXact Mini Spin Columns Profinity eXact™ Mini Spin Columns Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes Pkg of 10 mini spin columns packed with 0.1 ml Profinity eXact resin, includes 50 capped, collection tubes Pkg of 10, mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-3007_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-3007_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-3007_feature.jpg preview RepEx Profinity eXact Mini Spin Columns Pkg of 10 mini spin columns packed with 0.1 ml Profinity eXact Resin, includes 50 capped, collection tubes 1563007, profinity, exact, mini, spin, columns, purification, resin, affinity, tagged, affinity-tagged, chromatography, subtilisin, protease, conjugated, agarose-based, matrix 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Affinity-Chromatography-and-Ta N 0 <p>Choose Profinity eXact Mini Spin Columns for rapid purification and in-line tag removal of Profinity eXact-tagged proteins by affinity chromatography. The spin columns can be used with a standard centrifuge and one purification run takes less than one hour.</p> <ul> <li>Consists of a novel affinity medium immobilized with a modified version of the subtilisin protease</li> <li>Allows the highly specific protease to bind strongly to the Profinity eXact tag with sub-nanomolar affinity</li> <li>Helps cleave and elute the tag-free target protein from the column with its native or desired N-terminal amino acids</li> </ul> <h2 class="descHeader">Related Products</h2> <ul> <li>Profinity eXact Purification Resin (<a href="/en-us/sku/1563005-profinity-exact-purification-resin-10-ml">1563005</a>)</li> <li>Profinity eXact Mini Purification Starter Kit (<a href="/en-us/sku/1563006-profinity-exact-mini-spin-purification-starter-kit">1563006</a>)</li> <li>Bio-Scale Mini-Profinity eXact Cartridges, 1 ml (<a href="/en-us/sku/7324646-bio-scale-mini-profinity-exact-cartridges">7324646</a>)</li> <li>Bio-Scale Mini-Profinity eXact Cartridge, 5 ml (<a href="/en-us/sku/7324648-bio-scale-mini-profinity-exact-cartridge">7324648</a>)</li> </ul> <h2 class="descHeader">Related Cateogories</h2> <ul> <li><a href="/en-us/category/profinity-exact-fusion-tag-system">Profinity eXact Fusion-Tag System</a></li> <li> <a href="/en-us/product/profinity-exact-expression-vector-kits-cloning-products">Profinity eXact Expression Vector Kits and Cloning Products</a></li> <li> <a href="/en-us/product/profinity-exact-purification-tag-cleavage-consumables">Profinity eXact Purification and Tag Cleavage Consumables</a> <ul> </ul> </li> </ul> <h2 class="descHeader">Related Content</h2> <ul> <li><a href="/en-us/category/activated-affinity-media">Activated Affinity Media</a></li> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Quantitation</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <p>Profinity eXact Purification Resin <a href="/webroot/web/pdf/lsr/literature/Bulletin_5655.pdf" target="_blank">Information Sheet</a></p> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs/autopopulated 156-3007.html /webroot/web/html/lsr/products/SKUs/autopopulated/156-3007.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563007&CartQuote=QT&quantity= 1332 false true false $175.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563007&AddToDefaultWishList=true&quantity= true false true 41106500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1563007&quantity= 1563007
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Bio-Scale Mini Profinity eXact Cartridge

7324648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

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Bio-Scale Mini Profinity eXact Cartridges

7324647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

13 7324647 /templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4647_0921120619483.xml 7324647 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcv374gwjcv374gx" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv374gwjcv374gx"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4647_0921120619483.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="26" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="29" ZoneOffsetMillis="0" Time="1517000129920">Fri Jan 26 20:55:29 UTC 2018</LastExecutionTime> </Node> <Node Name="PROD"> <Deployment Id="StandardMetadata.jcv375apjcv375aq" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcv375apjcv375aq"> <Name>StandardMetadata</Name> <TargetEnviroment>PROD</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Instrument-Systems--Components/732-4647_0921120619483.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="27" DayOfWeek="6" Year="2018" Hour="1" Minute="21" Second="46" ZoneOffsetMillis="0" Time="1517016106731">Sat Jan 27 01:21:46 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:06 PM en_US jao0teqt 2017-11-14 07:08:06 true true internet/product_SKU LSR N en 09/21/19 12:06 PM 1517016309450 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:06 7324647-bio-scale-mini-profinity-exact-cartridges 7324647 Bio-Scale Mini Profinity eXact Cartridges Bio-Scale Mini Profinity eXact Cartridges Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4647_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4647_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4647_feature.jpg RepEx 732-4647 Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Instrument-Systems--Components N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324647&CartQuote=QT&quantity= 1332 false true false $404.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324647&AddToDefaultWishList=true&quantity= true false true 41115700 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324647&quantity= 7324647
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Bio-Scale Mini Profinity eXact Cartridges

7324646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

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Number Description Options
5655 Profinity eXact™ Resin Product Information Sheet, Rev A Click to download
5668 Profinity eXact Fusion-Tag System Brochure, Rev A Click to download [ Add to Cart (Free) ]
5646 Profinity eXact™ Cloning and Expression Kits, PIS, Rev B Click to download
5656 Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B Click to download
5652 Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A Click to download
10011163 Profinity eXact™ Mini Spin Columns Manual, Rev A Click to download
5766 Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A Click to download [ Add to Cart (Free) ]
5725 Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
10011260 Profinity eXact Protein Purification System Manual, Rev A Click to download

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