Profinity Exact™ Purification Resin

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Profinity Exactâ„¢ Purification Resin

Overview

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to bulletin 3193 for purification conditions.
** For example, Profinity GST resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Profinity eXact Purification Resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The resulting purity of the eluted protein is typically higher than that for other affinity-tag systems due to the specific recognition (KD < 100 pM) that subtilisin has for its prodomain sequence (the Profinity eXact Tag). Once the Profinity eXact fusion protein has been captured by the Profinity eXact Purification Resin — and the cleavage reaction has been triggered with a sodium fluoride-containing buffer — the affinity tag is precisely cleaved at the C-terminus of the cleavage recognition sequence (EEDKLFKAL). The result is a quickly purified protein product that lacks any amino acid residues derived from the Profinity eXact Tag.

Features and Benefits of Profinity eXact Resin

  • Purification and processing of fusion-tagged proteins in a single-step
  • On-column cleavage in as little as 30 min
  • No protease addition required
  • Precise cleavage at N-terminus to generate native protein sequence

The following convenient packaging configurations allow easy evaluation of Profinity eXact technology:

  • Profinity eXact Resin — 10 ml affinity resin supplied in 100 mM sodium phosphate, pH 7.2 containing 0.02% sodium azide
  • Profinity eXact Mini Spin Columns — 10-pack of single-use columns that contain 0.1 ml settled resin in addition to all required collection tubes for binding, washing, and elution steps. Fit all benchtop centrifuges
  • Profinity eXact Mini Spin Starter Kit — comprehensive 10-pack kit that ensures purification success upon initial trial. Includes 10 prepacked spin columns, collection tubes, lysis reagent, buffers for purification, and lyophilized MBP control protein lysate
  • Bio-Scale Mini Profinity eXact Cartridges — 1 ml and 5 ml cartridges that can be used with BioLogic chromatography systems, the Profinia™ Protein Purification System, a peristaltic pump, or with appropriate column adaptors with any other chromatography system
  • Profinity eXact Expression and Purification Starter Kit — includes two kits:
    • 1 Profinity eXact Cloning and Expression Starter Kit for the cloning and expression of a target gene using pPAL vectors and competent cells
    • 1 Profinity eXact Mini Spin Purification Starter Kit for single-step purification and on-column cleavage of the tagged protein

More Information

Profinity eXact™ Resin Product Information Sheet (Bulletin 5655)

Profinity eXact™ Fusion-Tag System Brochure (Bulletin 5668)

Profinity eXact™ Protein Purification System Manual (Bulletin 10011260)

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Profinity eXact Purification Resin

1563005
10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide

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$535.00
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(Discontinued) Profinity eXact Expression and Purification Starter Kit

1563008
Kit includes 1 Profinity eXact cloning and expression starter kit (20 reactions) and 1 Profinity eXact mini spin purification starter kit (10 spin columns)

This product is no longer available. Please contact technical support for more information on replacement product.
Profinity eXact Mini Spin Purification Starter Kit

1563006
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers

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$470.00
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Number Description Options
5655 Profinity eXact™ Resin Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5668 Profinity eXact Fusion-Tag System Brochure, Rev A Click to download [ Add to Cart (Free) ]
5646 Profinity eXact™ Cloning and Expression Kits, PIS, Rev B Click to download [ Add to Cart (Free) ]
5656 Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B Click to download [ Add to Cart (Free) ]
5652 Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A Click to download [ Add to Cart (Free) ]
10011163 Profinity eXact™ Mini Spin Columns Manual, Rev A Click to download
5766 Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A Click to download [ Add to Cart (Free) ]
5725 Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
10011260 Profinity eXact Protein Purification System Manual, Rev A Click to download [ Add to Cart (Free) ]