| |
Matrix |
Functional Group |
Specificity |
Capacity |
Working pH |
Pressure Limit |
Applications |
| Ready-to-Use Affinity Media |
| Nuvia™ IMAC |
High capacity, pressure-stable polymer based on UNOsphere™ beads |
NTA charged with Ni2+ |
Histidine |
≥40 mg/ml* |
2–14 |
45 psi
(3.1 bar) |
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals |
| Profinity™ IMAC |
Pressure-stable polymer based on UNOsphere beads |
IDA, available charged with Ni2+ and uncharged |
Histidine |
≥15 mg/ml* |
1–14 |
100 psi
(6.8 bar) |
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals |
| Profinity GST** |
Pressure-stable polymer based on UNOsphere beads |
Immobilized glutathione |
GST-tagged proteins |
≥10 mg/ml |
1–14 |
45 psi
(3.1 bar) |
Purification of recombinant GST-tagged proteins |
| Profinity eXact™*** |
Crosslinked 6% agarose |
Subtilisin protease |
Subtilisin prodomain |
≥3 mg/ml tag-free protein |
2–10 |
10 psi (minus system pressure) |
Generation of native, tag-free proteins by on-column purification and cleavage |
| Affi-Gel® Protein A |
Crosslinked agarose |
Protein A
2 mg/ml |
IgG |
|
2–10 |
15 psi
(1 bar) |
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible |
| Affi-Prep® Protein A |
Pressure-stable polymer |
Protein A
2 mg/ml |
IgG |
|
2–10 |
1,000 psi (70 bar) |
Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications |
| Affi-Gel Blue |
Crosslinked agarose |
Cibacron Blue F3GA 1.9 mg/ml |
Albumin; general |
≥11 mg/ml |
2–10 |
15 psi
(1 bar) |
Binds many nucleotide-requiring enzymes, albumin, and other proteins |
| DEAE Affi-Gel Blue |
Crosslinked agarose |
Cibacron Blue F3GA and DEAE |
Albumin and serum proteins |
0.14 ml serum/ml gel |
2–10 |
15 psi
(1 bar) |
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation |
| CM Affi-Gel Blue |
Crosslinked agarose |
Cibacron Blue F3GA and CM |
Albumin and serum proteins |
0.17–0.5 ml serum/ml gel |
2–11 |
15 psi
(1 bar) |
Produces albumin- and protease-free antibody preparation from serum without prior dialysis |
| Affi-Prep Polymyxin |
Pressure-stable polymer |
Polymyxin 2–4 mg/ml |
Endotoxins |
>5 mg/ml |
2–10 |
1,000 psi
(70 bar) |
Endotoxin removal |
| Affi-Gel Boronate |
Polyacrylamide gel |
Boronate 1.05 ± 0.15 |
cis-diols
meq/g |
130 µmol sorbitol/ml |
2–10 |
15 psi
(1 bar) |
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides |
| Activated Media for Spontaneous Ligand Immobilization |
| Profinity Epoxide |
Pressure-stable polymer based on UNOsphere beads |
Epoxy group |
Nucleophiles; amini, thiol, -COOH |
36–40 mg/ml lgG |
1–14 |
Up to 80 psi
(5.5 bar) |
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins) |
| Affi-Gel 10 |
Crosslinked agarose |
N-hydroxy-succinimide ≥10 µmol/ml |
-NH2 |
35 mg/ml |
3–11 |
15 psi
(1 bar) |
For coupling proteins with pI 6.5–11 |
| Affi-Gel 15 |
Crosslinked agarose |
N-hydroxy-succinimide ≥9 µmol/ml |
-NH2 |
35 mg/ml |
3–11 |
15 psi
(1 bar) |
For coupling proteins with pI 6.5–11 |
| Affi-Gel Hz |
Crosslinked agarose |
Hydrazide |
Oxidized carbohydrates |
1–5 mg/ml |
2–10 |
15 psi
(1 bar) |
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules |
| Affinity Media Using Carbodiimide Activation |
| Affi-Gel 102 |
Crosslinked agarose |
-NH2 16 ± 4 meq/ml |
-COOH |
40 mg |
2–11 |
15 psi
(1 bar) |
Carbodiimide coupling of carboxyl-containing ligand |
