Profinity Epoxide Resin

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Profinity Epoxide Resin

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Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

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Profinity epoxide resin is an activated affinity chromatography support for the immobilization of ligands that contain nucleophiles, such as amino, thiol, or hydroxyl groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.

Profinity epoxide resin is based on UNOsphere beads, which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. The open pore structure allows coupling of large ligands for the purification of large targets. Examples include protein A for purification of monoclonal antibodies, and other ligands for purification of recombinant proteins, such as those containing maltose-binding protein and calmodulin. In addition, Profinity epoxide allows operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs. It is supplied as a dry powder (1 g of powder gives ~8 ml final volume).

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Profinity™ Epoxide Resin, 5 g

1560200
5 g, activated affinity chromatography support for immobilization of biomolecules

0 1560200 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0200_0921120656996.xml 1560200 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US juso9m6f 2018-02-01 00:17:45 true true internet/product_SKU juso9m6f templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0200_0921120656996.xml LSR 2019-05-04 18:20:19 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348629 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:19 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0200_0921120656996.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0200_0921120656996.xml 1560200-profinity-epoxide-resin-5-g 1560200 Profinity Epoxide Resin Profinity™ Epoxide Resin, 5 g 5 g, activated affinity chromatography support for immobilization of biomolecules 5 g, activated affinity chromatography support for immobilization of biomolecules 5 g, activated affinity chromatography support for immobilization of biomolecules /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-0200_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-0200_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-0200_feature.jpg preview ,VN RepEx 156-0200 5 g, activated affinity chromatography support for immobilization of biomolecules 1560200, profinity, epoxide, resin, activated, affinity, chromatography, support, media, unosphere, beads, immobilization, biomolecules, ligands, purification, protein, carbohydrate, dna, 5g 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose activated Profinity Epoxide Resin for covalent coupling of ligands to a macroporous support that permits high flow rates. This resin can be used with batch protocols, spin columns or on gravity, low-pressure, and medium-pressure chromatography systems.</p> <ul> <li>Couples molecules that contain nucleophiles such as amino, thiol and hydroxyl groups</li> <li>Beads have open pore structure permitting the coupling of large ligands and purification of large molecules and complexes including antibodies</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>1 g powder produces 5.5&ndash;8.0 ml of settled bed</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>Profinity Epoxide Resin is also available in a 25 g package (<a href="/en-us/sku/1560201-profinity-epoxide-resin-25-g?ID=1560201">1560201</a>)</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a>, hold up to 2 ml support</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a>, hold up to 20 ml support</li> <li><a href="/en-us/product/empty-spin-columns?ID=612dbf40-5acd-468f-8eef-b855ce941865">Spin Columns</a></li> <li><a href="/en-us/product/profinia-desalting-kits">Profinia Desalting Kits </a></li> </ul> <h2 class="descHeader">Related Content</h2> <ul> <li><a href="/en-us/category/activated-affinity-media">Activated Affinity Media</a></li> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Quantitation</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity Epoxide Resin <a href="/webroot/web/pdf/lsr/literature/10003245A.PDF" target="_blank">Instruction Manual</a></li> <li>Premade Buffer Kits, Media, and Prepacked Cartridges for Affinity Purification <a href="/webroot/web/pdf/lsr/literature/Bulletin_5925B.pdf" target="_blank">Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-epoxide-resin-5g-1560200.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-epoxide-resin-5g-1560200.html Functional group 1667 Epoxy 1 0 Form 1659 Powder, 1 g hydrates to 5.5–8 ml 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Functional groups 1668 50–132 µmol/g resin 1 0 Maximum operating pressure 1817 80 psi (5.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature 1 0 false $135.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560200&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560200&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560200&AddToDefaultWishList=true&quantity= 13111000 135.00 1560200
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Profinity™ Epoxide Resin, 25 g

1560201
25 g, activated affinity chromatography support for immobilization of biomolecules

1 1560201 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0201_0921120656973.xml 1560201 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US juso9m6e 2018-02-01 00:17:45 true true internet/product_SKU juso9m6e templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0201_0921120656973.xml LSR 2019-05-04 18:20:18 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556917348678 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 18:20:18 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0201_0921120656973.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0201_0921120656973.xml 1560201-profinity-epoxide-resin-25-g 1560201 Profinity Epoxide Resin Profinity™ Epoxide Resin, 25 g 25 g, activated affinity chromatography support for immobilization of biomolecules 25 g, activated affinity chromatography support for immobilization of biomolecules 25 g, activated affinity chromatography support for immobilization of biomolecules /webroot/web/images/ps/products/affinity_media/sku_thumb/global/156-0201_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/156-0201_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/156-0201_feature.jpg preview ,VN RepEx 156-0201 25 g, activated affinity chromatography support for immobilization of biomolecules 1560201, profinity, epoxide, resin, activated, affinity, chromatography, support, media, unosphere, beads, immobilization, biomolecules, ligands, purification, protein, carbohydrate, dna, 25g 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose activated Profinity Epoxide Resin for covalent coupling of ligands to a macroporous support that permits high flow rates. This resin can be used with batch protocols, spin columns or on gravity, low-pressure, and medium-pressure chromatography systems.</p> <ul> <li>Couples molecules that contain nucleophiles such as amino, thiol and hydroxyl groups</li> <li>Beads have open pore structure permitting the coupling of large ligands and purification of large molecules and complexes including antibodies</li> <li>Stable across a wide pH range (1&ndash;14)</li> <li>1 g powder produces 5.5&ndash;8.0 ml of settled bed</li> </ul> <h2 class="descHeader">Packaging Options</h2> <p>Profinity Epoxide Resin is also available in a 5 g package (<a href="/en-us/sku/1560200-profinity-epoxide-resin-5-g?ID=1560200">1560200</a>)</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Empty Bio-Scale Mini Cartridges, 1 ml (<a href="/en-us/sku/7324660-1-ml-empty-bio-scale-mini-cartridges">7324660</a>) and 5 ml (<a href="/en-us/sku/7324661-5-ml-empty-bio-scale-mini-cartridges">7324661</a>)</li> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a>, hold up to 2 ml support</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a>, hold up to 20 ml support</li> <li><a href="/en-us/product/empty-spin-columns?ID=612dbf40-5acd-468f-8eef-b855ce941865">Spin Columns</a></li> <li><a href="/en-us/product/profinia-desalting-kits">Profinia Desalting Kits </a></li> </ul> <h2 class="descHeader">Related Content</h2> <ul> <li><a href="/en-us/category/activated-affinity-media">Activated Affinity Media</a></li> <li><a href="/en-us/category/ngc-medium-pressure-liquid-chromatography-systems?ID=MFCV7415">NGC&trade; Medium-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems</a></li> <li><a href="/en-us/category/profinia-affinity-chromatography-protein-purification-system">Profinia&trade; Affinity Chromatography Protein Purification System</a></li> <li><a href="/en-us/category/protein-assay-kits-cuvettes">Protein Quantitation</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Profinity Epoxide Resin <a href="/webroot/web/pdf/lsr/literature/10003245A.PDF" target="_blank">Instruction Manual</a></li> <li>Premade Buffer Kits, Media, and Prepacked Cartridges for Affinity Purification <a href="/webroot/web/pdf/lsr/literature/Bulletin_5925B.pdf" target="_blank">Information Sheet</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> <p>&nbsp;</p> /webroot/web/html/lsr/products/SKUs/autopopulated profinity-epoxide-resin-25g-1560201.html /webroot/web/html/lsr/products/SKUs/autopopulated/profinity-epoxide-resin-25g-1560201.html Functional group 1667 Epoxy 1 0 Form 1659 Powder, 1 g hydrates to 5.5–8 ml 1 0 Particle size 2763 45–90 μm (mean 60 μm) 1 0 Functional groups 1668 50–132 µmol/g resin 1 0 Maximum operating pressure 1817 80 psi (5.5 bar) 1 0 Recommended flow rate 2058 ≤600 cm/hr at 25°C 1 0 Operating temperature range 2553 4–40°C 1 0 Autoclavability (121°C, 30 min) 1355 Yes (in 0.1 M sodium acetate) 1 0 Storage 2198 4°C or ambient 1 0 Shelf life 2149 ≥1 year at ambient temperature 1 0 false $644.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560201&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560201&CartQuote=QT&quantity= 1332 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560201&AddToDefaultWishList=true&quantity= 13111000 644.00 1560201
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