Profinia Protein Purification System Kits

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Overview

Profinia Purification and Buffer Selection Guide
   
Buffer Sets
Powdered
Reagents
Bio-Scale Mini
Cartridges*
         
 
Control Lysate
Lysis and Wash
Desalting
Cleaning and Storage
Urea
Glutathione
Affinity
Desalting
 
IMAC Kits                
Native IMAC starter kit
   
IMAC purification kit  
   
IMAC buffer kit  
       
GST Kits                
GST starter kit
 
GST purification kit  
 
GST buffer kit  
 
   
Desalting Kits                
Desalting purification kits    
     
Desalting buffer kit    
       
 
Profinia Purification Kits
Profinia Purification Kits

Profinia purification kits come with prepacked cartridges, buffers, and cleaning and storage reagents required for purification.

Profinia Buffer Kits
Profinia Buffer Kits

Profinia buffer kits contain buffers and cleaning and storage reagents required for purification.

Profinia Starter Kits
Profinia Starter Kits

Profinia starter kits contain buffers and reagents, cartridges, and a control lysate required for purification.

Profinia kits are used in a variety of protein purification methods, such as IMAC, GST, and desalting, carried out on the Profinia protein purification system. Three main kit configurations are available:

  • Profinia purification kits
  • Profinia starter kits
  • Profinia buffer kits

Profinia purification kits are supplied with a set of prepacked Bio-Scale Mini cartridges (for IMAC, GST, or desalting), application-specific purification buffers, and cleaning and storage reagents. Profinia starter kits contain a set of purification cartridges and application-specific buffers and reagents, but are also supplied with a control lysate to facilitate system setup and initial purification. Profinia buffer kits are an appropriate choice if you require only additional buffers and cleaning and storage reagents.

Profinia consumables are optimally formulated and ready to use, minimizing the time required to obtain a purified sample. Typical yields and recovery from a single purification run on a 1 ml cartridge are:

  • ≥10 mg protein in ~35 min for native IMAC
  • ≥10 mg protein in ~20 min for denaturing IMAC
  • ≥10 mg protein in ~50 min for GST

All reagents included in these kits are made with chromatography-grade solutions and have been prefiltered through a 0.2 µm filter. These reagents are specially formulated to work with the Profinia instrument. They will allow you to automatically carry out equilibration, binding, washing, and elution steps as well as necessary cartridge maintenance procedures, such as cartridge cleaning and storage.

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Profinia IMAC Purification Kit

6200225
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges

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$500.00
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Profinia IMAC Purification Kit

6200235
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)

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$855.00
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Profinia GST Purification Kit

6200226
Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 2 each of 1 ml GST and 10 ml desalting cartridges

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$555.00
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Profinia GST Purification Kit

6200236
Kit for use with the Profinia protein purification system, includes 1 each of 5 ml GST and 50 ml desalting cartridges, 2 Profinia GST buffer kits (#620-0223)

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$1,060.00
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Profinia Desalting Purification Kit

6200228
Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges

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$255.00
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Profinia Desalting Purification Kit

6200238
Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)

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$640.00
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Profinia IMAC Buffer Kit

6200221
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

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$340.00
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Profinia GST Buffer Kit

6200223
Buffers, solutions, and glutathione sufficient for 10 applications, for use with the Profinia protein purification system

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$380.00
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Desalting and Cartridge Cleaning Buffer Kit

6200224
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

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$230.00
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Profinia IMAC Starter Kit

6200229
Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 1 each of 1 ml IMAC and 10 ml desalting cartridges, E. coli lysate

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$515.00
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Profinia GST Starter Kit

6200230
Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 1 each of 1 ml GST and 10 ml desalting cartridges, E. coli lysate, glutathione

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$495.00
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Number Description Options
10006044 Instruction Manual, Profinia Purification and Buffer Kits, Rev D Click to download
10006057 Instruction Manual, Profinia IMAC and GST Starter Kits, Rev E Click to download
10015932 Profinia™ Protein Purification System FAQs, Rev B Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]