Prestained Natural Protein Standards

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Prestained Natural Protein Standards

Overview

Kaleidoscope Standards

Kaleidoscope prestained standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots.


Kaleidoscope prestained standards
Calibrated Molecular Weights of Kaleidoscope Prestained Standards.
Protein Color MW (kD)
Myosin Blue 216.0
β-Galactosidase Magenta 132.0
BSA Green 78.0
Carbonic anhydrase Violet 45.7
Soybean trypsin inhibitor Orange 32.5
Lysozyme Red 18.4
Aprotinin Blue 7.6


MWs are of representative lots; actual weights may vary. Lot-specific MWs are included with each vial.

Prestained SDS-PAGE Standards
Prestained SDS-PAGE Standards


Use prestained SDS-PAGE standards to assess the efficiency of electrophoretic transfer on western blots. Broad range prestained SDS-PAGE standards, 5 µl, were run on a 4–20% Ready Gel® precast gel and transferred to nitrocellulose in the Mini Trans-Blot® cell. Bio-Rad's original prestained SDS-PAGE standards are available in high, low and broad ranges.


Calibrated Molecular Weights of Prestained SDS-PAGE Standards.
  Ranges Available
Protein MW (kD) Broad Low High
Myosin 210.0  
β-Galactosidase 125.0  
Phosphorylase b 113.0    
Bovine serum albumin (BSA) 101.0
Ovalbumin 56.2
Carbonic anhydrase 35.8  
Soybean trypsin inhibitor 29.0  
Lysozyme 21.0  
Aprotinin 6.9    


MWs are of representative lots; actual weights may vary. Lot-specific MWs areincluded with each vial.

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Prestained Nautral Standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. Each lot of Kaleidoscope and SDS-PAGE prestained natural protein standards is individually calibrated for estimating the MW of sample proteins.

Kaleidoscope™ Natural Protein Standards

Kaleidoscope Prestained Natural Standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots.

  Calibrated Molecular Weights* of Kaleidoscope Protein Standards

Protein Color Prestained
Myosin Blue 216,000
β-Galactosidase Magenta 132,000
BSA Green 78,000
Carbonic anhydrase Violet 45,700
Soybean trypsin inhibitor Orange 32,500
Lysozyme Red 18,400
Aprotinin Blue 7,600

* MWs are of representative lots; actual weights may vary. Lot-specific MWs are included with each vial.

Prestained Natural SDS-PAGE Protein Standards

Original Bio-Rad Prestained Protein Standards for SDS-PAGE are available in broad, low, and high ranges.

  Calibrated Molecular Weights* of Prestained Natural SDS-PAGE Standards

Protein Broad Range Low Range High Range
Myosin 209,000 204,000
β-Galactosidase 124,000 123,000
Phosphorylase b 103,000
BSA 80,000 77,000 80,000
Ovalbumin 49,100 50,000 48,000
Carbonic anhydrase 34,800 34,300
Soybean trypsin inhibitor 28,900 28,800
Lysozyme 20,600 20,700
Aprotinin 7,100

* MWs are of representative lots; actual weights may vary. Lot-specific MWs are included with each vial.

MW size range Varies by standard
Stain type Stained
Volume 500 µl
Shelf life 12 months
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Prestained SDS-PAGE Standards, broad range, 500 µl

1610318
500 μl, broad range standards (7,100–209,000 MW), prestained mixture of eight proteins

This product is not available for sale online. Please contact customer service for more information.

The following replacement is recommended:

  • Precision Plus Protein™ All Blue Prestained Protein Standards (1610373)
Number Description Options
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev D Click to download
2414 The Little Book of Standards, Rev D Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
5561 Precision Plus Protein™ WesternC™ Standards, Rev E Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download
4006026 Instruction Manual, Prestained SDS-PAGE Standards, Broad Range, Rev D Click to download
4006024 Instruction Manual, Prestained SDS-PAGE Standards, Low Range, Rev D Click to download
4006025 Instruction Manual, Prestained SDS-PAGE Standards, High Range, Rev B Click to download
LIT599 Instruction Manual, Kaleidoscope™ Prestained Protein Standards, Rev E Click to download
4006029 Instruction Manual, Kaleidoscope™ Polypeptide Standard, Rev B Click to download
2000 Polypeptide Standards: SDS-PAGE and Kaleidoscope Standards PIS, Rev B Click to download

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