Premixed Nucleic Acid Electrophoresis and Sample Buffers

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Premixed Electrophoresis Running Buffers
Save preparation time and standardize your electrophoresis runs with premixed electrophoresis running buffers. They can be used with handcast or precast gels. Premixed buffers are made with Bio-Rad electrophoresis-purity reagents and are quality controlled to ensure reproducible results. The 5 L cube packages are economical and are designed with an easy-pour spout for convenience. They are compact and stackable to save bench space.

Premixed Electrophoresis Buffer Composition

Buffer1x FormulationApplication(s)
10x TBE 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3 Nucleic acid electrophoresis/sequencing; polyacrylamide or agarose gels
10x TBE extended range*130 mM Tris, 45 mM boric acid, 2.5 mM EDTA, pH 8.3Nucleic acid electrophoresis
50x TAE40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0Polyacrylamide or agarose gels

* Extends buffer capacity for longer DNA sequencing runs. Buffers are made with 18 megaohm water.

Premixed Electrophoresis Sample Buffers
Simplify and standardize your sample loading with premixed electrophoresis sample buffers. The concentrated formulas allow these buffers to be used with both liquid and lyophilized samples. All premixed sample buffers are tested to ensure quality and consistency.

Premixed Sample Buffer Composition

BufferFormulationApplication(s)
1x TBE-urea sample buffer 89 mM Tris-HCl, pH 8.0, 89 mM boric acid, 2 mM EDTA, 7 M urea, 12% Ficoll, 0.01% bromophenol blue, 0.02% xylene cyanole FF Denaturing, ssDNA, RNA
5x nucleic acid sample buffer50 mM Tris-HCl, pH 8.0, 25% glycerol, 5 mM EDTA, 0.2% bromophenol blue, 0.2% xylene cyanole FFNondenaturing, dsDNA

Premixed Electrophoresis Running Buffer Specifications  
Buffer 1x formulation
10x TBE 89 mM Tris, pH 8.3, 89 mM boric acid, 2 mM EDTA
10x TBE extended range 130 mM Tris, pH 8.3, 45 mM boric acid, 2.5 mM EDTA
50x TAE 40 mM Tris, pH 8.0, 20 mM acetic acid, 1 mM EDTA
Premixed Electrophoresis Sample Buffer Specifications  
Buffer Formulation
Composition 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.0, 12% ficoll, 0.01% bromophenol blue, 0.02% xylene cyanol FF, 7 M urea
Storage 4°C
Shelf life 6 months
50x Tris/Acetic Acid/EDTA (TAE)

161-0773
5 L cube, premixed nucleic acid electrophoresis buffer, pH 8.0

List Price:   $235.00
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10x Tris/Boric Acid/EDTA (TBE)

161-0770
5 L cube, premixed nucleic acid electrophoresis buffer, pH 8.3

List Price:   $107.00
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10x Tris/Boric Acid/EDTA (TBE)

161-0741
1 L, premixed buffer for extended range DNA sequencing runs, pH 8.3

List Price:   $55.00
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50x Tris/Acetic Acid/EDTA (TAE)

161-0743
1 L, premixed nucleic acid electrophoresis buffer, pH 8.0

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10x Tris/Boric Acid/EDTA (TBE)

161-0733
1 L, premixed nucleic acid electrophoresis buffer, pH 8.3

List Price:   $36.00
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TBE-Urea Sample Buffer

161-0768
30 ml, 1x TBE-urea sample loading buffer, premixed, for denaturing ssDNA and RNA, pH 8.0

List Price:   $55.00
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Nucleic Acid Sample Loading Buffer

161-0767
10 ml, 5x DNA loading buffer, premixed, for nondenaturing TBE gels, pH 8.0

List Price:   $20.00
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Number Description Options
4106116 Instruction Manual, Nucleic Acid Sample Loading Buffer, Rev C Click to download
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]