Premixed Casting Buffers

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Premixed Casting Buffers

Overview

Gel Casting Buffers
Solution Applications
1.5 M Tris-HCL, pH 8.8 Resolving gel preparation
0.65 M Tris-HCL, pH 6.8 Stacking gel preparation
Acrylamide Solutions
19:1 Acrylamide/Bis DNA sequencing
29:1 Acrylamide/Bis Protein separation
37.5:1 Acrylamide/Bis Protein separation
Blotting Buffer Guide
  1 x Formulation Applications
Transfer Buffers*
10 Tris/glycine
25mM Tris, 192 mM glycine, pH 8.3 Western Blotting
10x Tris CAPS Anode buffer; 60 mM Tris,
40 mM CAPS, 15% methanol, pH 9.6
A discontinuous buffer system that increases ransfer efficiency in semi-dry applications
  Cathode buffer, 60 mM Tris,
40 mM CAPS, 0.1% (w/v) SDS, pH 9.6
 
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Capillary transfer of agarose gels
Processing Buffers
10x PBS
10 mM sodium phosphate,
150 mM NaCl, pH 7.4
Western blotting wash solution
10x TBS 20 mM Tris,
500 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting wash solution
1x PBS with 1% casein 10 mM sodium phosphate,
150 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS with 1% casein 20 mM Tris, 200 mM NaCl, containing 1% (w/v) casein, pH 7.4 Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Northern and Southern blotting prehybridization and hybridization solutions
*These buffers can be used for all gel types and formulations.
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Bio-Rad offers a variety of prepared solutions for casting polyacrylamide gels:

  • Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Use the 0.5 M Tris-HCl (pH 6.8) buffer for stacking gels and the 1.5 M Tris-HCl (pH 8.8) buffer for resolving gels
  • Acrylamide solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing conditions allow acrylamide solutions to be stable for 1 year at 4°C. Bio-Rad acrylamide solutions are available in two concentrations (30% and 40%) and in three acrylamide/bis ratios (19:1, 29:1, and 37.5:1)
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Resolving Gel Buffer for PAGE

1610798
1 L, 1.5 M Tris-HCl, pH 8.8

0 1610798 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0798_0921120415095.xml 1610798 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1610798-resolving-gel-buffer-for-page 1610798 Resolving Gel Buffer Resolving Gel Buffer for PAGE 1 L, 1.5 M Tris-HCl, pH 8.8 1 L, 1.5 M Tris-HCl, pH 8.8 1 L, 1.5 M Tris-HCl, pH 8.8 /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0798_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0798_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0798_feature.jpg Resolving Gel Buffer for PAGE preview RepEx 161-0798 1 L, 1.5 M Tris-HCl, pH 8.8 161-0798, 1610798, resolving, gel, buffer, protein, electrophoresis, casting, resolve, native, sds, page, gels 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Resolving Gel Buffer when casting your own polyacrylamide protein gels. This buffer is used to cast the resolving (sieving) portion of SDS or native gels.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Stacking Gel Buffer (<a href="/en-us/sku/1610799-stacking-gel-buffer-for-page?ID=1610799-for-page">1610799</a>)</li> <li><a href="/en-us/product/acrylamide-solutions-powders?ID=5e7a4f31-879c-4d63-ba0b-82556a0ccf1d">Acrylamide Solutions and Powders</a></li> <li>TEMED (<a href="/en-us/sku/1610800-temed">1610800</a>)</li> <li>Ammonium Persulfate (APS) (<a href="/en-us/sku/1610700-ammonium-persulfate-aps">1610700</a>)</li> <li>10x Tris/Glycine/SDS (<a href="/en-us/sku/1610732-10x-tris-glycine-sds?ID=1610732">1610732</a>)</li> <li>10x Tris/Glycine (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734">1610734</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/product/polyacrylamide-gel-reagents/tgx-fastcast-acrylamide-solutions">TGX&trade; FastCast&trade; Acrylamide Solutions</a></li> <li><a href="/en-us/category/polyacrylamide-gel-reagents?ID=ce5bc208-a990-4043-ad17-e7bd874bf301">Polyacrylamide Gel Reagents</a></li> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/protein-extraction">Protein Extraction</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Ready-to-Run Buffers and Solutions <a href="/webroot/web/pdf/lsr/literature/Bulletin_2317.pdf" target="_blank">Brochure</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610798.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610798.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610798&CartQuote=QT&quantity= false true false $29.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610798&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610798&quantity= 1610798
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Stacking Gel Buffer for PAGE

1610799
1 L, 0.5 M Tris-HCl, pH 6.8

1 1610799 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0799_0921120415076.xml 1610799 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1610799-stacking-gel-buffer-for-page 1610799 Stacking Gel Buffer Stacking Gel Buffer for PAGE 1 L, 0.5 M Tris-HCl, pH 6.8 1 L, 0.5 M Tris-HCl, pH 6.8 1 L, 0.5 M Tris-HCl, pH 6.8 /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0799_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0799_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0799_feature.jpg Stacking Gel Buffer for PAGE preview RepEx 161-0799 1 L, 0.5 M Tris-HCl, pH 6.8 161-0799, 1610799, stacking, gel, buffer, premixed, casting, cast, protein, electrophoresis, polyacrylamide, page 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Stacking Gel Buffer when casting your own polyacrylamide protein gels. This buffer is used to cast the stacking portion of SDS or native gels.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Resolving Gel Buffer (<a href="/en-us/sku/1610798-resolving-gel-buffer-for-page?ID=1610798-for-page">1610798</a>)</li> <li><a href="/en-us/product/acrylamide-solutions-powders?ID=5e7a4f31-879c-4d63-ba0b-82556a0ccf1d">Acrylamide Solutions and Powders</a></li> <li>TEMED (<a href="/en-us/sku/1610800-temed">1610800</a>)</li> <li>Ammonium Persulfate (APS) (<a href="/en-us/sku/1610700-ammonium-persulfate-aps">1610700</a>)</li> <li>10x Tris/Glycine/SDS (<a href="/en-us/sku/1610732-10x-tris-glycine-sds?ID=1610732">1610732</a>)</li> <li>10x Tris/Glycine (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734">1610734</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/product/polyacrylamide-gel-reagents/tgx-fastcast-acrylamide-solutions">TGX&trade; FastCast&trade; Acrylamide Solutions</a></li> <li><a href="/en-us/category/polyacrylamide-gel-reagents?ID=ce5bc208-a990-4043-ad17-e7bd874bf301">Polyacrylamide Gel Reagents</a></li> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/protein-extraction">Protein Extraction</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Ready-to-Run Buffers and Solutions <a href="/webroot/web/pdf/lsr/literature/Bulletin_2317.pdf" target="_blank">Brochure</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610799.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610799.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610799&CartQuote=QT&quantity= false true false $29.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610799&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610799&quantity= 1610799
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$29.00 
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2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download

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