Premixed Casting Buffers

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Overview

Gel Casting Buffers
Solution Applications
1.5 M Tris-HCL, pH 8.8 Resolving gel preparation
0.65 M Tris-HCL, pH 6.8 Stacking gel preparation
Acrylamide Solutions
19:1 Acrylamide/Bis DNA sequencing
29:1 Acrylamide/Bis Protein separation
37.5:1 Acrylamide/Bis Protein separation
Blotting Buffer Guide
  1 x Formulation Applications
Transfer Buffers*
10 Tris/glycine
25mM Tris, 192 mM glycine, pH 8.3 Western Blotting
10x Tris CAPS Anode buffer; 60 mM Tris,
40 mM CAPS, 15% methanol, pH 9.6
A discontinuous buffer system that increases ransfer efficiency in semi-dry applications
  Cathode buffer, 60 mM Tris,
40 mM CAPS, 0.1% (w/v) SDS, pH 9.6
 
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Capillary transfer of agarose gels
Processing Buffers
10x PBS
10 mM sodium phosphate,
150 mM NaCl, pH 7.4
Western blotting wash solution
10x TBS 20 mM Tris,
500 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting wash solution
1x PBS with 1% casein 10 mM sodium phosphate,
150 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS with 1% casein 20 mM Tris, 200 mM NaCl, containing 1% (w/v) casein, pH 7.4 Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Northern and Southern blotting prehybridization and hybridization solutions
*These buffers can be used for all gel types and formulations.

Bio-Rad offers a variety of prepared solutions for casting polyacrylamide gels:

  • Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Use the 0.5 M Tris-HCl (pH 6.8) buffer for stacking gels and the 1.5 M Tris-HCl (pH 8.8) buffer for resolving gels
  • Acrylamide solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing conditions allow acrylamide solutions to be stable for 1 year at 4°C. Bio-Rad acrylamide solutions are available in two concentrations (30% and 40%) and in three acrylamide/bis ratios (19:1, 29:1, and 37.5:1)
Resolving Gel Buffer

161-0798
1 L, 1.5 M Tris-HCl, pH 8.8

List Price:   $27.00
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Stacking Gel Buffer

161-0799
1 L, 0.5 M Tris-HCl, pH 6.8

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2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]