Premixed Blotting Buffers

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Premixed Blotting Buffers
Premixed Blotting Buffers

Blotting transfer buffers must allow effective elution of proteins or nucleic acids from the gel matrix as well as their effective binding to membranes.

Blotting Buffer Guide
  1 x Formulation Applications
Transfer Buffers*
10 Tris/glycine
25mM Tris, 192 mM glycine, pH 8.3 Western Blotting
10x Tris CAPS Anode buffer; 60 mM Tris,
40 mM CAPS, 15% methanol, pH 9.6
A discontinuous buffer system that increases ransfer efficiency in semi-dry applications
  Cathode buffer, 60 mM Tris,
40 mM CAPS, 0.1% (w/v) SDS, pH 9.6
 
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Capillary transfer of agarose gels
Processing Buffers
10x PBS
10 mM sodium phosphate,
150 mM NaCl, pH 7.4
Western blotting wash solution
10x TBS 20 mM Tris,
500 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting wash solution
1x PBS with 1% casein 10 mM sodium phosphate,
150 mM NaCl, 1% (w/v) casein, pH 7.4
Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS with 1% casein 20 mM Tris, 200 mM NaCl, containing 1% (w/v) casein, pH 7.4 Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes)
20x SSC 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0
Northern and Southern blotting prehybridization and hybridization solutions
*These buffers can be used for all gel types and formulations.

Blotting transfer buffers must allow effective elution of proteins or nucleic acids from the gel matrix as well as their effective binding to membranes. Three premixed transfer buffers are available: 10x Tris/glycine, 10x Tris/CAPS, and 20x SSC.

Processing blots for protein and nucleic acid detection is very simple with premixed wash buffers and blocking solutions. Premixed wash buffers in PBS, TBS, and SSC reduce the number of stock solutions to prepare. Premixed blocking buffers, available as 1x PBS with casein and 1x TBS with casein, take the time and effort out of solubilizing casein.

10x Tris/Glycine

161-0734
1 L, 10x premixed electrophoresis buffer

List Price:   $27.00
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10x Tris/Glycine

161-0771
5 L cube, 10x premixed electrophoresis buffer

List Price:   $88.00
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10x Tris/CAPS

161-0778
1 L, 10x Tris/CAPS

List Price:   $69.00
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20x SSC

161-0774
1 L, 20x SSC, blot transfer and processing buffer, pH 7.0

List Price:   $22.00
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20x SSC

161-0775
5 L cube, 20x SSC, blot transfer and processing buffer, pH 7.0

List Price:   $95.00
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10x Phosphate Buffered Saline

161-0780
1 L, 10x PBS

List Price:   $41.00
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10x Tris Buffered Saline

170-6435
1 L, 10x TBS

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Number Description Options
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]