Precision Plus Protein™ Standard Plugs

Precision Plus Protein™ Standard Plugs

Overview

Precision Plus Protein unstained standard plugs allow easy, quick, and clean loading of molecular weight standards on any gel. The plugs can be used on vertical 2–D gels with no reference well.
Read full description

Description

Description

Precision Plus Protein standard plugs allow easy, quick, and clean loading of molecular weight standards on any gel. The plugs can be used on vertical 2-D gels with no reference well. Precision Plus Protein unstained standards are cast in 1 mm thick agarose plugs for easy storage, handling, and loading. Load concentrations have been optimized for SYPRO Ruby, Silver Stain Plus™, and Bio-Safe™ Coomassie Blue staining.

Some features and benefits of the plugs are:

  • Precast for ease of use — just snap, twist, and load the plug onto a gel
  • Absolute molecular weight accuracy, confirmed by mass spectrometry
  • Ideal ten-band range from 10 kD to 250 kD, with 3 high-intensity reference bands (25 kD, 50 kD, and 75 kD) for instant gel orientation
  • Molecular weights that never change, so band sizes are easy to remember
  • High r2 value, >0.995, allowing accurate molecular weight determination
  • Known concentration of protein in each band, allowing quantitation capability
  • Strep-tag amino acid sequence for detection and molecular weight determination on western blots

 

Precision Plus Protein standard plugs come in easy-to-use snap-off molds in packs of 24 (one application per plug).

Specifications

Specifications

MW size range
10 kD – 250 kD
Stain type
Unstained
Volume
24 Plugs
Number of applications
24
Plug buffer
1% agarose, 128 mM glycine,
10% (w/v) glycerol,
0.7% SDS, 62.5 mM Tris pH8.3,
50 mM DTT, 5 mM EDTA,
0.02% NaN3,
0.003% bromophenol blue
Shelf life at 4°C, months
3

Ordering

Ordering

items

Use the filters below to refine results!

Image
161-0378_tn.jpg
1610378
Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands

Documents

Documents

Number Description Options
6533
Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A
2414
The Little Book of Standards, Rev D
2998
Protein Standards Application Guide, Rev G
[ Add to Cart (Free) ]
3036
Precision Plus Protein Plugs Promotional Card, Rev C
4117684
Instruction Manual, Precision Plus Protein™ Standard Plugs, Unstained, Rev C
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
[ Add to Cart (Free) ]