Precision Plus Protein™ Standard Plugs

Print

Overview

Using Precision Plus Protein™ Standard Plugs

Step 1
Break and twist off one end of the plastic plug casing.

Step 2
Break off an individual plastic plug from the casing by placing pressure on the flat side to break the junction.

Step 3
Complete the break by twisting off the individual plastic plug casing.

Step 4
Snap off the attached spatula.

Step 5
Use the spatula to push the plug out of the plastic casing.

Step 6
Use the spatula to slide the plug outward and on to the top plate of your gel.

Step 7
Slide the plug all the way down the bottom of the well next to IPG strip. Then add an agarose overlay.



Second dimension SDS-PAGE gel run with a mouse brain sample, Precision Plus Standards Plugs, and stained with Bio-Safe™ coomassie G-250 stain.

Unstained Standards

Precision Plus Protein unstained standards on a gel. These standards have been engineered to contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots.

Precision Plus Protein™ Standard Plugs

Precision Plus Protein standards packaged in an easy-to-use plug for 2-D electrophoresis.

Plug Run on a PROTEAN Plus Gel

Precision Plus Protein Standard plug on a 12% PROTEAN Plus precast gel. Sample is mouse liver.

Precision Plus Protein standard plugs allow easy, quick, and clean loading of molecular weight standards on any gel. The plugs can be used on vertical 2–D gels with no reference well. Precision Plus Protein unstained standards are cast in 1 mm thick agarose plugs for easy storage, handling, and loading. Load concentrations have been optimized for SYPRO Ruby, Silver Stain Plus, and Bio-Safe Coomassie Blue staining.

Some features and benefits of the plugs are:

  • Precast for ease of use — just snap, twist, and load the plug onto a gel
  • Absolute molecular weight accuracy, confirmed by mass spectrometry
  • Ideal ten-band range from 10 kD to 250 kD, with 3 high-intensity reference bands (25 kD, 50 kD, and 75 kD) for instant gel orientation
  • Molecular weights that never change, so band sizes are easy to remember
  • High r2 value, >0.995, allowing accurate molecular weight determination
  • Known concentration of protein in each band, allowing quantitation capability
  • Strep-tag amino acid sequence for detection and molecular weight determination on western blots

 

Precision Plus Protein standard plugs come in easy-to-use snap-off molds in packs of 24 (one application per plug).

MW size range 10 kD – 250 kD
Stain type Unstained
Volume 24 Plugs
Number of applications 24
Plug buffer 1% agarose, 128 mM glycine,
10% (w/v) glycerol,
0.7% SDS, 62.5 mM Tris pH8.3,
50 mM DTT, 5 mM EDTA,
0.02% NaN3,
0.003% bromophenol blue
Shelf life at 4°C, months 3
Precision Plus Protein™ Standard Plugs
Precision Plus Protein Standard Plugs

161-0378
Pkg of 24, 1 mm thick agarose plugs containing 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands

List Price:   $115.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6533 Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A Click to download
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
2998 Protein Standards Application Guide, Rev F Click to download [ Add to Cart (Free) ]
3036 Precision Plus Protein Plugs Promotional Card, Rev C Click to download
4117684 Instruction Manual, Precision Plus Protein Standard Plugs, Unstained, Rev C Click to download
5890 Did You Know Precision Plus Insert Rev B Click to download