Criterion™ TGX Stain-Free™ Precast Gels

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Overview

Ordering Information
  Criterion™ TGX Stain-Free™ Gels
  12+2-Well 45 µl 18-Well 30 µl 26-Well 15 µl Prep+2-Well 800 µl IPG+1-Well 11 cm IPG Strip
 
Resolving Gel % 12+2-Well
45 µl
18-Well
30 µl
26-Well
15 µl
Prep+2-Well
800 µl
IPG+1-Well
11 cm IPG Strip
 
7.5% 567-8023 567-8024 567-8025
10% 567-8033 567-8034 567-8035
12% 567-8043 567-8044 567-8045
18% 567-8073 567-8074 567-8075 567-8072 567-8071
4–15% 567-8083 567-8084 567-8085 567-8082 567-8081
4–20% 567-8093 567-8094 567-8095 567-8092 567-8091
8–16% 567-8103 567-8104 567-8105 567-8102 567-8101
10–20% 567-8113 567-8114 567-8115 567-8112 567-8111
Any kD™ 567-8123 567-8124 567-8125 567-8122 567-8121
 
Criterion™ TGX Stain-Free™ Precast Gels Migration Chart
Chart
 
Performance Data

 

Superior resolution of a variety of samples. Precision Plus Protein™ unstained standards were loaded in a volume of 5 µl in each lane of a 4–20% Criterion™ TGX Stain-Free™ gel. The gel was run in a Criterion™ cell at 200 V for 45 min. A, protein bands were visualized using the Criterion Stain Free™ imager; B, the separated proteins were then transferred on to an Immun-Blot® PVDF membrane using Towbin buffer (with 20% methanol) in a Criterion blotter at 100 V for 1 hr. The transferred proteins on the membrane were also visualized using the Criterion Stain Free imager to confirm transfer efficiency; C, the gel after transfer was checked for non-transferred proteins; D, the blot was probed against 1:20,000 dilution of Streptactin-HRP conjugate, developed with Immun-Star™ Western C™ substrate, and visualized on a Molecular Imager® ChemiDoc™ XRS system.

*The Criterion Stain Free imaging system has been replaced by the Gel Doc™ EZ imaging system, which has the same performance characteristics for imaging stain-free gels but has a broader range of imaging applications.

Criterion TGX Interactive Gel Selector

The new Criterion™ TGX (Tris-Glycine eXtended) Stain-Free™ precast gels for PAGE are based on the long shelf life TGX formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with the Gel Doc™ EZ and ChemiDoc™ MP imaging systems.*

Benefits include:

  • Fast run times with protein separation, gel imaging, and complete analysis in less than 30 min
  • Rapid fluorescent detection with the Gel Doc EZ and ChemiDoc MP imaging systems
  • Comparable sensitivity to Coomassie stain
  • Better reproducibility and quantitation compared to other staining procedures
  • Inexpensive Laemmli buffer system, low running costs
  • Protein transfer in as little as 15 min
  • Same gel can be used for staining, western blotting, or mass spectrometry

TGX Stain-Free gels retain Laemmli-like separation characteristics using standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min using the Criterion electrophoresis cell and then visualized with the Gel Doc EZ and ChemiDoc MP imagers in as little as 2.5 min. The trihalo compounds react with tryptophan residues in a UV-induced reaction to produce fluorescence, which can be easily detected by the Gel Doc EZ and ChemiDoc MP imagers within gels or on a PVDF membrane after transfer.

*These gels can also be imaged on the discontinued Criterion Stain Free™ imaging system, which has been replaced by the Gel Doc EZ and ChemiDoc MP imaging systems.

 

Gel dimensions (W x L x thickness) 133 x 87 x 1 mm
Cassette dimensions (W x L x thickness) 150 x 106 x 53 mm
Cassette material Styrene copolymer
Comb material Polycarbonate
Gel storage conditions Store flat at 4°C; do not freeze
Shelf life at 2–8°C* 12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample) 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/Glycine/SDS) 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Run times at constant voltage: 200 V 42–50 min
Run times at constant voltage: 300 V 20–26 min
* From date of manufacture.  
7.5% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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7.5% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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7.5% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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12% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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12% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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12% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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18% Criterion™ TGX Stain-Free™ Gel, 11 cm IPG/prep+1 well, 550 µl

567-8071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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18% Criterion™ TGX Stain-Free™ Gel, Prep+2 well, 700 µl

567-8072
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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18% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8073
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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18% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8074
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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18% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8075
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–15% Criterion™ TGX Stain-Free™ Gel, 11 cm IPG/prep+1 well, 550 µl

567-8081
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–15% Criterion™ TGX Stain-Free™ Gel, Prep+2 well, 700 µl

567-8082
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–15% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–15% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–15% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–20% Criterion™ TGX Stain-Free™ Gel, 11 cm IPG/prep+1 well, 550 µl

567-8091
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–20% Criterion™ TGX Stain-Free™ Gel, Prep+2 well, 700 µl

567-8092
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–20% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–20% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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4–20% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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8–16% Criterion™ TGX Stain-Free™ Gel, 11 cm IPG/prep+1 well, 550 µl

567-8101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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8–16% Criterion™ TGX Stain-Free™ Gel, Prep+2 well, 700 µl

567-8102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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8–16% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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8–16% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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8–16% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10–20% Criterion™ TGX Stain-Free™ Gel, 11 cm IPG/prep+1 well, 550 µl

567-8111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10–20% Criterion™ TGX Stain-Free™ Gel, Prep+2 well, 700 µl

567-8112
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10–20% Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8113
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10–20% Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8114
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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10–20% Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8115
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Any kD™ Criterion™ TGX Stain-Free™ Gel, 11 cm IPG/prep+1 well, 550 µl

567-8121
Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Any kD™ Criterion™ TGX Stain-Free™ Gel, Prep+2 well, 700 µl

567-8122
Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Any kD™ Criterion™ TGX Stain-Free™ Gel, 12+2 well, 45 µl

567-8123
Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Any kD™ Criterion™ TGX Stain-Free™ Gel, 18 well, 30 µl

567-8124
Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Any kD™ Criterion™ TGX Stain-Free™ Gel, 26 well, 15 µl

567-8125
Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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Number Description Options
6427 Bio-Rad V3 Western Workflow, Rev A Click to download [ Add to Cart (Free) ]
10040758 Instruction Manual, Image Lab Software: Imaging Stain-Free Gels and Blots, Rev B Click to download
6434 Western Blot Normalization Using Image Lab™ Software Quick Start Guide Click to download
6507 TGX and TGX Stain-Free Gels: Publications List Click to download
0512 Evaluation of Stain-Free Gels for Gel-MS Applications (Poster) Click to download
5974 Mini-PROTEAN and Criterion TGX Stain-Free Precast Gels, Rev D Click to download [ Add to Cart (Free) ]
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
5810 Compatibility of the Criterion Stain Free Gel Imaging System with Mass Spectrometric Protein Analysis, Rev A Click to download
5981 Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart, Rev B Click to download [ Add to Cart (Free) ]
6008 Comparison of the Criterion TGX Stain-Free Precast Gel System and Standard Coomassie Staining Procedures for Running and Imaging Protein Gels, Rev A Click to download [ Add to Cart (Free) ]
6032 Life Technologies Bis-Tris and Tris-Glycine to Criterion TGX/TGX Stain-Free Precast Gels Conversion Chart, Rev B Click to download [ Add to Cart (Free) ]
6088 V3 Western Workflow Brochure with Gel Doc EZ Imager, Rev B Click to download [ Add to Cart (Free) ]
6166 Multiplex Fluorescent Blot Detection: A Troubleshooting Guide, Rev A Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion Gel Application Guide, Rev F Click to download
RP0051 Stain-Free Approach for Western Blotting Click to download
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download [ Add to Cart (Free) ]
6453 Utilize Stain-Free Technology in a Variety of Workflows, Rev B Click to download