Polyacrylamide Reagents and Precast Gels

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Additional Product Descriptions

Premixed Buffers for Protein Electrophoresis
Save preparation time and ensure perfect electrophoresis results every time. Just dilute and run using our premixed 10x buffer concentrates. Our buffers are made with electrophoresis-purity reagents and are quality-controlled to ensure reproducible results. Electrophoresis buffers are available in two formulations:

  • Tris/glycine/SDS buffer for standard vertical polyacrylamide gel electrophoresis (SDS-PAGE) applications
  • Tris/glycine buffer for native or nondenaturing PAGE

Premixed Sample Buffer for Protein Electrophoresis
Simplify sample preparation and save time! Bio-Rad's Laemmli sample loading buffer contains the electrophoresis tracking dye Bromophenol Blue, SDS, glycerol, and Tris buffer. Add directly to samples to solubilize proteins, boil, then load your gels with ease.

Tris-HCI Gels for SDS-PAGE and Native PAGE of Proteins
Ready Gel Tris-HCl gels are formulated without SDS, allowing them to be used for either native PAGE or SDS-PAGE applications. They are available either as singlepercentage acrylamide or acrylamide gradient gels. Single-percentage gels are cast with a 4% stacking gel to sharpen protein bands into thin zones before they enter the resolving, or separating, gel. Linear gradient gels offer superior resolution and definition of samples containing a wide range of molecular weights.

TBE Gels for Nucleic Acids
Ready Gel TBE gels provide rapid, high-resolution separations of nucleic acids from 50 to 1,750 base pairs. This range is ideal for analyzing the purity of PCR fragments. Acrylamide-based Ready Gel TBE gels provide higher resolution than agarose gels and require no preparation.

Choosing the Right Precast Gel

Migration charts
Use these protein and nucleic acid migration charts to select an acrylamide percentage that will provide optimal resolution of your samples. On single-percentage gels, optimal separation occurs in the lower half of the gel. Gradient gels optimize separation along the entire length of the gel. When selecting a single-percentage gel, choose the percentage in which your sample's proteins of interest will migrate to the lower half of the gel.

If your sample contains a limited size range of molecules, a single-percentage gel will provide the best separation. In general, singlepercentage gels will best separate bands that are close in molecular weight.

If your sample contains a broad range of molecular weights, a gradient gel allows both high and low molecular weight bands to be resolved on the same gel. Molecules with a range of sizes can be separated on linear gradient gels because the larger pore sizes allow resolution of larger molecules, while the decreasing pore sizes toward the bottom of the gel restrict excessive separation of small molecules.

Migrations are based on the dye front being run to the bottom of the gel.

Mini-PROTEAN® TGX™ Precast Gels


Ready Gel® Precast Gels

Protein migration on Ready Gel Tris-HCI precast gels. Percentages refer to acrylamide percentages. The molecular mass of each band is given in kD.

Nucleic acid migration on Ready Gel TBE precast gels. Percentages refer to acrylamide percentages. Bands are labeled by number of bp. XC = Xylene Cyanole FF, BPB = Bromophenol Blue.

Precast Gel Features

Ready Gel 10-Well Gel

Ready Gel 15-Well Gel

Bio-Rad Convenience for You

Ready Gel precast gels are available with different combs

  • 10-well combs are most popular in teaching labs and accommodate samples of up to 30 �l
  • 15-well combs are designed for samples of up to 20 �l
Outlined sample wells
After you remove the combs, the sample wells are outlined on the outside of the precast gel cassettes to simplify sample loading and eliminate loading errors. Only from Bio-Rad!

 

Premixed Buffers for Electrophoresis

Bio-Rad pioneered the production of reagents for electrophoresis. Our convenient premixed electrophoresis buffers are the perfect solutions to your classroom electrophoresis and blotting needs.

Mini-PROTEAN® TGX Precast Polyacrylamide Gels

The long shelf life TGX precast gels are a part of an innovative gel system designed to provide Laemmli-like separation patterns using a standard Tris/glycine running buffer system. TGX precast gels are compatible with the Mini-PROTEAN® electrophoresis cells. View the Ready Gel® to Mini-PROTEAN® TGX Precast Gels conversion chart.

Benefits include:

  • Fast run times
  • 12 month shelf life at 4ºC
  • Usable with existing buffer systems
  • Bottom-open cassette design for simple gel handling and blotting
  • Gel opening lever (456-0000), sold separately, is 100% aluminum and recyclable

Ready Gel® Precast Polyacrylamide Gels

Ready Gel polyacrylamide precast gels are designed to fit the Mini-PROTEAN® Tetra cell and are ready to run. Simply lock them into the cell, load your samples, and get sharp, beautifully resolved protein bands in 30–45 min.

Our precast gels provide high quality and reliability and eliminate exposure to toxic unpolymerized acrylamide. Ready Gel precast gels are available in a wide selection of formulations for SDS-PAGE, native PAGE, peptide separations, and nucleic acid separations.

7.5% Mini-PROTEAN® TGX™ Precast Gel #456-1023EDU
7.5% Mini-PROTEAN® TGX™ Precast Gel

456-1023EDU
Pkg of 10, 7.5% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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Mini-PROTEAN TGX, 10%, 10W, 10
10% Mini-PROTEAN® TGX™ Precast Gel

456-1033EDU
Pkg of 10, 10% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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10% Mini-PROTEAN® TGX™ Precast Gel
10% Mini-PROTEAN® TGX™ Precast Gel

456-1036EDU
Pkg of 10, 10% precast polyacrylamide gel, 15-well, 15 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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12% Mini-PROTEAN® TGX™ Precast Gel
12% Mini-PROTEAN® TGX™ Precast Gel

456-1043EDU
Pkg of 10, 12% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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12% Mini-PROTEAN® TGX™ Precast Gel
12% Mini-PROTEAN® TGX™ Precast Gel

456-1043SEDU
Pkg of 2, 12% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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4–15% Mini-PROTEAN® TGX™ Precast Gel
4–15% Mini-PROTEAN® TGX™ Precast Gel

456-1083EDU
Pkg of 10, 4–15% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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4–15% Mini-PROTEAN® TGX™ Precast Gel
4–15% Mini-PROTEAN® TGX™ Precast Gel

456-1083SEDU
Pkg of 2, 4–15% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells

List Price:   $22.00
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4–20% Mini-PROTEAN® TGX™ Precast Gel
4–20% Mini-PROTEAN® TGX™ Precast Gel

456-1093SEDU
Pkg of 2, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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4–20% Mini-PROTEAN® TGX™ Precast Gel
4–20% Mini-PROTEAN® TGX™ Precast Gel

456-1093EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

List Price:   $103.00
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4–20% Mini-PROTEAN® TGX™ Precast Gel
4–20% Mini-PROTEAN® TGX™ Precast Gel

456-1096EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 15-well, 15 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

List Price:   $103.00
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Any kD™ Mini-PROTEAN® TGX™ Precast Gel
Any kD™ Mini-PROTEAN® TGX™ Precast Gel

456-9033EDU
Pkg of 10, Any kD precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; includes 1 opening lever; education use only

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40% Acrylamide/Bis Solution, 19:1

161-0145EDU
2 x 500 ml, 40% acrylamide and bis-acrylamide solution, 19:1; education use only

List Price:   $128.00
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Hazardous  
Ammonium Persulfate

161-0700EDU
10 g, ammonium persulfate (APS) catalyst; education use only

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Hazardous  
TEMED

161-0800EDU
5 ml, catalyst for polyacrylamide gels, hazardous shipping charges may apply; education use only

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Tris-HCl Ready Gel Precast Gel

161-1100EDU
Pkg of 1, 7.5% precast polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1101EDU
Pkg of 1, 10% precast polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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(Discontinued) Tris-HCl Ready Gel Precast Gel

161-1102EDU
Pkg of 1, 12% precast polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

This product is no longer available. Please contact customer service for more information on replacement product.
Tris-HCl Ready Gel Precast Gel

161-1103EDU
Pkg of 1, 15% precast polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1104EDU
Pkg of 1, 4–15% precast gradient polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1105EDU
Pkg of 1, 4–20% precast gradient polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Ready Gel Tris-HCl Gel

161-1210EDU
Pkg of 1, 5% precast polyacrylamide gel, 10-well, 30 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1118EDU
Pkg of 1, 7.5% precast polyacrylamide gel, 15-well, 15 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1119EDU
Pkg of 1, 10% precast polyacrylamide gel, 15-well, 15 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1120EDU
Pkg of 1, 12% precast polyacrylamide gel, 15-well, 15 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1121EDU
Pkg of 1, 15% precast polyacrylamide gel, 15-well, 15 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1122EDU
Pkg of 1, 4–15% precast gradient polyacrylamide gel, 15-well, 15 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Tris-HCl Ready Gel Precast Gel

161-1123EDU
Pkg of 1, 4–20% precast gradient polyacrylamide gel, 15-well, 15 µl, 8 x 10 cm (W x L), for use with Mini-PROTEAN Tetra cells; education use only

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Number Description Options
5932 Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E Click to download [ Add to Cart (Free) ]
5871 Mini-PROTEAN TGX Precast Gels Product Information Sheet, Rev F Click to download [ Add to Cart (Free) ]
5910 Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B Click to download
5911 Mini-PROTEAN TGX Precast Gel: A Versatile and Robust Laemmli-Like Precast Gel for SDS-PAGE, Rev A. Click to download
LIT300 Instruction Manual, Piperazine Diacrylamide (PDA), Rev B Click to download
1156 Acrylamide Polymerization — A Practical Approach Click to download
1866 Acrylamide Brochure Click to download
LIT492 Instruction Manual, Acrylamide and bis-Acrylamide Solutions Click to download
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]
5535 Mini-PROTEAN Electrophoresis System Brochure, Rev E Click to download [ Add to Cart (Free) ]
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel System Resource Guide (Note: large PDF file - 3.8 MB) Click to download
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download
2376 Gel Electrophoresis: Separation of Native Basic Proteins by Cathodic, Discontinuous Polyacrylamide Gel Electrophoresis Click to download