TGX Stain-Free™ FastCast™ Acrylamide Solutions

Print
TGX Stain-Free FastCast Acrylamide Solutions

Overview

TGX™ FastCast™ Tutorial Video

See how easily and quickly you can cast gels with TGX FastCast acrylamide kits. This tutorial video walks you through the steps for casting mini gels with the Mini-PROTEAN® Tetra cell casting module.


With TGX FastCast acrylamide solutions you can:

  • Save time by casting both resolver and stacker simultaneously
  • Store gels for up to 4 weeks after casting (at 4°C)
  • Run gels in 20 min and transfer proteins in 3 min
  • Visualize proteins in less than 5 min without staining
TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kits: Tips & Tricks

TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks

Follow this list of bench-tested tips for trouble-free gel casting.

Download PDF


 
FastCast™ Kits Gel Calculator

The FastCast kits calculator makes mixing and casting TGX™ and TGX Stain-Free™ FastCast gels even easier with less chance of error. Just select the gel size and number of gels you want to cast, and the volumes of stacker, resolver, APS, and TEMED are automatically calculated.

Download FastCast Calculator

Mini-PROTEAN Tetra Cell Bundle Selection Guide
Mini-PROTEAN Tetra Systems 165-8025 165-8026 165-8027 165-8028 165-8029 165-8033 165-8035 165-8030 165-8034 165-8036
General                    
Mini-PROTEAN Tetra cell
Gel system 4-Gel 4-Gel 4-Gel 4-Gel 4-Gel 4-Gel 4-Gel Precast (4-Gel) Precast (4-Gel) Precast (4-Gel)
Power supply PP* Basic PP* Universal PP* HC PP* HV PP* Basic PP* HC PP* Basic PP* HC
Blot module Mini Trans-Blot® Mini Trans-Blot Mini Trans-Blot Mini Trans-Blot Mini Trans-Blot Mini Trans-Blot
System Components
                   
Electrode assembly (I) 1 1 1 1 1 1 1 1 1 1
Companion running module 1 1 1 1 1 1 1 1 1 1
Tank 1 1 1 1 1 1 1 1 1 1
Lid with power cables 1 1 1 1 1 1 1 1 1 1
Mini cell buffer dam 1 1 1 1 1 1 1 1 1 1
Hand Casting Components                    
Wells 10 10 10 10 10 10 10
Thickness 1.0 mm 1.0 mm 1.0 mm 1.0 mm 1.0 mm 1.0 mm 1.0 mm
Combs (10-well) 5 5 5 5 5 5 5
Glass plates 5 5 5 5 5 5 5
Casting stands 2 2 2 2 2 2 2
Casting frames 4 4 4 4 4 4 4
Sample loading guides (10-well) 1 1 1 1 1 1 1
Blot Module Components                    
Modular electrode assembly 1 1 1 1 1 1
Gel holder cassettes 2 2 2 2 2 2
Fiber pads 4 4 4 4 4 4
Bio-Ice cooling unit 1 1 1 1 1 1

*PowerPac™ power supply

Electrophoresis System Selection Guide

Overview of Vertical Electrophoresis Cells
The Mini-PROTEAN Tetra, Criterion, PROTEAN, and PROTEAN Plus systems all consist of electrophoresis cells, blotting equipment, precast gels, and power supplies.These systems provide:

  • A choice of 4 size formats with options to meet your specific needs for resolution, capacity, and processing speed
  • Cell designs that eliminate current leakage, to provide the most reproducible gels and consistent run times
  • Dodeca cells for high-throughput 2-D separations in mini, midi, and large format gel sizes
Format Advantages Compatible Gel
Sizes (W x L)
Electrophoresis Cells
Mini • Fastest turnaround of 2-D data for "2-D in a day" capability
• Useful for discovery projects,screening new samples, andevaluating sample preparationconditions
• Minimizes reagent cost and waste
• Improved design for error-free operation
• 1–4 gels in mini format
8.6 x 6.8 cm (precast)
8.3 x 7.3 cm (handcast)
Midi • Optimal combination of pI separation and fast run times
• Fast setup with "drop-in" gel and cell design
• 30% more pI separation than mini gels
• Capability for <1 hr second-dimension runs for "2-D in a day" results
13.3 x 8.7 cm (precast or handcast)
Large • Maximum resolution in a single gel
• Longest range of molecular weight separation
PROTEAN II xi/XL system
16 x 16 cm (precast or
handcast)
16 x 20 cm (handcast)
18.3 x 19.3 cm (precast)
18.3 x 20 cm (handcast)

PROTEAN Plus Dodeca cell

18.5 x 20 cm (handcast)
20 x 20.5 cm (handcast)
25 x 20.5 cm (handcast)
V3 Western Workflow™

TGX Stain-Free FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels. TGX (Tris-Glycine eXtended) formulation retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. TGX Stain-Free FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems.

TGX Stain-Free chemistry enables rapid fluorescent detection of proteins directly within gels and blots with Bio-Rad's stain-free enabled imagers. Protein samples can be separated in 20 minutes and then visualized in 5 minutes.

Features and Benefits of TGX Stain-Free FastCast Acrylamide Solutions

  • Handcast gels ready to run in as little as 30 min
  • Long shelf life — handcast gels remain fresh for up to 4 weeks at 4°C
  • Stain-free visualization of proteins in your gel or blot in less than 5 min with stain-free enabled imaging systems (Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ MP, and ChemiDoc XRS+)
  • Electrophoresis run times as short as 20 min using the Mini-PROTEAN® Tetra or Criterion™ cells
  • Efficient protein transfers in as little as 3 min using the Trans-Blot® Turbo™ transfer system
  • Ability to monitor the success of your electrophoresis and transfer steps without additional staining

Applications and Uses of TGX Stain-Free FastCast Acrylamide Solutions

  • 1-D polyacrylamide gel electrophoresis for separation of proteins
  • Accurate molecular weight estimation
  • Robust separation for complex samples

Approximate number of gels per kit.

  Mini Gels (~9 x 7 cm) Midi Gels (~13 x 9 cm)
  Mini-PROTEAN
Cassettes
0.75 mm
Glass Plates
1.0 mm
Glass Plates
1.5 mm
Glass Plates
Criteron
Cassettes
Starter Kit 11 gels 19 gels 13 gels 9 gels 7 gels
Full Kit 55 gels 95 gels 65 gels 45 gels 35 gels

More Information

TGX FastCast tutorial video Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guideLearn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.


 

FastCast kits calculator Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Stain-Free Imaging

Bio-Rad's V3 Western Workflow™ is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins. View the V3 Western Workflow video to learn more.

The Quick Start Guide to Stain-Free Imaging provides step-by-step instructions on how to obtain stain-free gel images from TGX Stain-Free gels in less than 5 minutes using Bio-Rad’s ImageLab™ software and stain-free enabled imagers.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.

Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Criterion and Criterion™ Dodeca™ cells use integral cassettes for gel hand casting. 1.0 mm thick gel casting sets are available in various comb configurations:

TGX Stain-Free? FastCast Acrylamide Starter Kit, 7.5%
TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 7.5%

161-0180
Starter kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

List Price:   $55.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
TGX Stain-Free FastCast Acrylamide Kit, 7.5%
TGX Stain-Free™ FastCast™ Acrylamide Kit, 7.5%

161-0181
Kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

List Price:   $198.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
TGX Stain-Free FastCast Acrylamide Starter Kit, 10%
TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10%

161-0182
Starter kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

List Price:   $55.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
TGX Stain-Free FastCast Acrylamide Kit, 10%
TGX Stain-Free™ FastCast™ Acrylamide Kit, 10%

161-0183
Kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

List Price:   $198.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
TGX Stain-Free FastCast Acrylamide Starter Kit, 12%
TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 12%

161-0184
Starter kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

List Price:   $55.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
TGX Stain-Free FastCast Acrylamide Kit, 12%
TGX Stain-Free™ FastCast™ Acrylamide Kit, 12%

161-0185
Kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

List Price:   $198.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6545 Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks, Rev A Click to download
6533 Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A Click to download
10033918 Instruction Manual, TGX™ and TGX Stain-Free™ FastCast™ Acrylamide Kit and Starter Kit Click to download
6455 FastCast Acrylamide Kits and Handcasting Equipment Brochure Click to download
6502 TGX and TGX Stain-Free FastCast Acrylamide Kits Brochure, Rev B Click to download
6507 TGX and TGX Stain-Free Gels: Publications List Click to download
10040758 Quick Start Guide to Stain-Free Imaging Click to download
0512 Evaluation of Stain-Free Gels for Gel-MS Applications (Poster) Click to download
6008 Comparison of the Criterion TGX Stain-Free Precast Gel System and Standard Coomassie Staining Procedures for Running and Imaging Protein Gels, Rev A Click to download [ Add to Cart (Free) ]
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]
RP0051 Stain-Free Approach for Western Blotting Click to download
RP0058 A Defined Methodology for Reliable Quantification of Western Blot Data Click to download
6494 TGX Stain-Free Gels with NGC Chromatography Systems , Rev B Click to download