The PharosFX and PharosFX Plus Imagers have been discontinued. However, we will continue to provide support for Pharos System owners until March, 2020.For many applications, camera-based chemiluminescence and chemifluorescence have now become viable replacements for laser scanning detection and imaging methods. We recommend that customers interested in purchasing an imager consider the ChemiDoc™ MP Imaging System or the ChemiDoc Touch Imaging System as alternatives to the Pharos System.
The PharosFX and PharosFX Plus Systems can be used to image many types of samples (such as gels, blots, and microplates) and to support a wide range of imaging applications. The product family covers a broad range of features, including:
- Enhanced sensitivity for multiplex fluorescence applications
- High sensitivity and precise quantitation for radioisotopes
- Flexibility in the choice of emission filters, including custom filters
- Ergonomic design for optimized workflow
- USB 2.0 interface for easy computer configurations and fast data transfer
The PharosFX and PharosFX Plus Systems use multiple lasers, which enhances application flexibility. Internal and external laser options allow optimal excitation of single- or multicolor fluorescent samples, permitting the detection of almost any fluorescent dye or label, including ethidium bromide, SYBR® Green I, SYBR® Gold, SYPRO Orange, SYPRO Red, Nile Red, SYPRO Ruby, Deep Purple, Alexa Fluor 488, 532, 546, and 635, FITC, FAM, Cy2, Cy3, Cy5, HEX, R6G, TAMRA, Texas Red, Pro-Q Diamond, and Pro-Q Emerald.
Software-controlled filter wheels accommodate eight filter slots, allowing detection of combinations of dyes. Installation of a custom emission filter allows detection of novel fluorophores with the PharosFX or PharosFX Plus System. For applications requiring additional laser lines, an optional external laser module (up to two lasers) can be added for more flexibility. Optional external 488 nm and 635 nm lasers are available for both systems. These upgrades allow users to change research focus or take advantage of developments in fluorescence chemistry.
All laser and filter combinations are driven by user-friendly Quantity One® 1-D Analysis Software. Simply choose the application, and the software automatically selects the optimal hardware (laser and filter) settings. You can also create custom applications and apply custom emission filters for novel fluorescent probes.