PharosFX™ and PharosFX Plus Systems

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Overview


PharosFX™ Systems Specifications
  Model Characteristics
 
  PharosFX™ Plus PharosFX
Detection limit      
  Storage phosphor <0.95 dpm/mm2 for 1 hr exposure to 14C using imaging screen-K
  <0.15 dpm/mm2 for 1 hr exposure to 32P using imaging screen-K
  Fluorescence (depends on experimental conditions) 0.2 fmol of FITC end-labeled DNA using 488 nm laser
 
6 pg of SYBR® Green I-stained DNA using 488 nm laser
  0.4 fmol of FITC end-labeled DNA using 532 nm laser
  25 pg of SYBR Green I-stained DNA using 532 nm laser
  0.2 fmol of Cy3 end-labeled DNA using 532 nm laser
  0.2 fmol Cy5 end-labeled DNA using 635 nm laser
Dynamic range 5 orders of magnitude (1:65,535) or 16-bit
Linearity r2 >0.99
Uniformity ±5% over entire scan area
Scan resolution 800, 200, 200, and 50 µm (user selectable)
Scan time 20 x 25 cm area; 8.5 min at 100 µm, 15 min at 50 µm
  35 x 43 cm area; 8.5 min at 200 µm, 17 min at 100 µm
Spatial resolution of storage phosphor* 14C:200 µm (2.5 line pairs/mm) using imaging screen-K

32P:300 µm (1.5 line pairs/mm) using imaging screen-K
Digital resolution 16-bit (65,636 gray scale)
Excitation source 25 mW 532 nm (green) diode-pumped solid-state laser
  10 mW 635 nm diode laser
Optional external lasers 15 mW 488 nm (blue) external argon ion laser
  10 mW 635 nm (red) external diode laser
Maximum power 65 W
Input voltage range 110–240 VAC, 50–60 Hz
Operating humidity requirements 10–32°C, 30–80%
Computer interface USB2
Operating system Windows 2000, XP, Vista, or Mac OS X
Dimensions (W x D x H) 57 x 68 x 30 cm (22.4 x 26.8 x 11.8")
Weight 32 kg (70.5 lb; scanner)

*Dependent on radioisotope characteristics and storage phosphor crystal size coated on the screen.

FITC/Cy3/Cy5-labeled DNA
FITC/Cy3/Cy5-labeled DNA

PharosFX™ and PharosFX™ Plus systems use multiple lasers, which enhances application flexibility. FITC/Cy3/Cy5-labeled DNA is shown here.

Flamingo Stain
DIGE With Cy2/Cy3/Cy5
14C-Labeled Rat

14C-labeled rat, longitudinal section (exposed to imaging screen-K)

35S-labeled 2-D PAGE Gel

35S-labeled 2-D PAGE gel (exposed to imaging screen-K).



The PharosFX™ and PharosFX™ Plus systems can be used to image many types of samples (such as gels, blots, and microplates) and to support a wide range of imaging applications. The product family covers a broad range of features, including:

  • Enhanced sensitivity for multiplex fluorescence applications
  • High sensitivity and precise quantitation for radioisotopes
  • Flexibility in the choice of emission filters, including custom filters
  • Ergonomic design for optimized workflow
  • USB2 interface for easy computer configurations and fast data transfer

Fluorescence Detection
The PharosFX and PharosFX Plus systems use multiple lasers, which enhances application flexibility. Internal and external laser options allow optimal excitation of single- or multicolor fluorescent samples, permitting the detection of almost any fluorescent dye or label, including ethidium bromide, SYBR® Green I, SYBR® Gold, SYPRO Orange, SYPRO Red, Nile Red, SYPRO Ruby, Deep Purple, Alexa Fluor 488, 532, 546, and 635, FITC, FAM, Cy2, Cy3, Cy5, HEX, R6G, TAMRA, Texas Red, Pro-Q Diamond, and Pro-Q Emerald.

Software-controlled filter wheels accommodate eight filter slots, allowing detection of combinations of dyes. Installation of a custom emission filter allows detection of novel fluorophores with the PharosFX or PharosFX Plus system. For applications requiring additional laser lines, an optional external laser module (up to two lasers) can be added for more flexibility. Optional external 488 nm and 635 nm lasers are available for both systems. These upgrades allow users to change research focus or take advantage of developments in fluorescence chemistry.

All laser and filter combinations are driven by user-friendly Quantity One® 1-D analysis software. Simply choose the application, and the software automatically selects the optimal hardware (laser and filter) settings. You can also create custom applications and apply custom emission filters for novel fluorescent probes.

Molecular Imager PharosFX™ Plus System

170-9460
110/240 V, PC or Mac, includes Quantity One software, sample tray set, 605DF50 and 640DF35 fluorescence and phosphor imaging filters, USB2 cable

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Molecular Imager PharosFX™ System

170-9450
110/240 V, PC or Mac, includes Quantity One® software, sample tray set, 605DF50 and 640DF35 fluorescence filters, USB 2 cable

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Number Description Options
5476 Molecular Imager PharosFX and PharosFX Plus Systems Folder Insert, Rev B Click to download
6126 Imaging Systems, Computer Specifications, Rev D Click to download
5888 Bio-Rad Imaging Systems Family Brochure, Rev E Click to download [ Add to Cart (Free) ]
2318 Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C Click to download
4000146 Instruction Manual, Molecular Imager FX, Custom Emission Filter Assemblies, Rev A Click to download
4000143 Instruction Manual, Molecular Imager FX, Multi-Sample Tray II, Rev B Click to download
4000126-14 Instruction Manual, The Discovery Series: Quantity One 1-D Analysis Software, Rev A Click to download
M4000093 Instruction Manual, PharosFX Molecular Imager System, Rev G Click to download
5460 Biomarker Discovery Workflow Brochure, Rev B Click to download
3179 Fluorescent Nanoparticles for Western Blotting, Rev A Click to download
5331 Molecular Imager PharosFX Systems Brochure, Rev B Click to download [ Add to Cart (Free) ]
5556 Protein Separation and Analysis Workflow Brochure, Rev B Click to download
3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download [ Add to Cart (Free) ]
3099A Expression Proteomics: Tools for Protein Sample Preparation, 2-D Electrophoresis, and Imaging and Analysis, Bulletins 3095, 3096, 3097 and 3098 [ Add to Cart (Free) ]