pGLO™ Bacterial Transformation Kit

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pGLO Bacterial Transformation Kit

Overview

Purchasing Guide Table

Reduce. Reuse. Refresh.

Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase.


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Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Plasmid (pGLO), lyophilized
1
E. coli strain HB101 K-12, lyophilized
1
LB nutrient broth, sterile 1
LB nutrient agar powder 1
Ampicillin, lyophilized 1
Arabinose, lyophilized 1
Sterile transformation solution (CaCl2) 1
Petri dishes, 60 mm, sterile 40
Inoculation loops, sterile 80
Micro test tubes, 2.0 ml, sterile, color coded 60
Foam micro test tube holders 8
Disposable plastic transfer pipets 50
UV Pen Light 1
Curriculum (printed manual not included); available as free download.
Recommended (Optional) Accessories:  
Incubation oven  
Water bath  
UV lamps
4–8
Microwave oven  
pGLO Bacterial Transformation Kit Workflow
pGLO Bacterial Transformation Kit Workflow
Key Kit Features
  • Aligns with AP Biology Lab 6
  • Transform bacteria with jellyfish gene
  • Turn the modified genes on or off
  • Study gene regulation
  • Complete in two 45 minute lab sessions
Curriculum Fit
Curriculum Fit
Gene Regulation

Gene regulation. With pGLO, students analyze the growth of bacteria on various media and examine the roles that external and internal factors play in gene regulation. Gene expression in all organisms is carefully regulated to allow adaptation to differing conditions and to prevent wasteful production of proteins. The bacterial genes encoding the enzymes needed to metabolize the simple sugar arabinose are a perfect example. A promoter region upstream of these genes acts as molecular on/off switch that regulates their expression. The genes are activated only when arabinose is present in the environment. Bio-Rad's pGLO plasmid incorporates the arabinose promoter, but the genes involved in the breakdown of arabinose have been replaced with the jellyfish gene encoding GFP. When bacteria transformed with pGLO are grown in the presence of arabinose, the GFP gene switches on, causing the bacteria to express GFP and to fluoresce brilliant green. When students genetically re-engineer bacteria with the genes from a bioluminescent jellyfish they never forget the central mantra of molecular biology:
DNA ⇒ RNA ⇒ Protein ⇒ Trait — Green Fluorescence!

Transformed bacteria are grown on a petri dish

Transformed bacteria are grown on a petri dish.

Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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Complete Purchasing Guide

Download the complete Biotechnology Explorer™ Refresh Kit Components Purchasing Guide.


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Bulletin 5872
PowerPoint Presentation for Workshops and for Teaching

pGLO Transformation and Green Fluorescent Protein Chromatography Kits

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the pGLO Transformation and Green Fluorescent Protein Chromatography Kits and includes instructions for using the kits.

pGLO Transformation and Green Fluorescent Protein Chromatography Kits Download PowerPoint presentation
pGLO Transformation and Green Fluorescent Protein Chromatography Kits

Genetic engineering is the process of manipulating the genetic material of an organism — often to include the DNA from a foreign organism. Using the pGLO™ Bacterial Transformation Kit, students transform bacteria by introducing a gene from the bioluminescent jellyfish Aequorea victoria. The same procedure has been used to create "designer proteins" which have led to the explosion of new health treatments, agricultural applications, and environmental solutions.

Features and Benefits

  • Aligns with AP Biology Big Idea 3, Lab 8
  • Transforms bacteria with a jellyfish gene
  • Turns the gene on and off for the study of gene regulation

Background Information

Bio-Rad's exclusive pGLO plasmid is constructed with the jellyfish gene that encodes green fluorescent protein (GFP), an antibiotic-resistance gene that encodes β-lactamase protein, and the araC gene encoding a regulator protein that turns the GFP gene on and off. Bacteria transformed with pGLO plasmid are selected by ampicillin resistance; when induced to express GFP, the bugs glow fluorescent green under UV light.

How It Works

With this activity, students analyze the growth of bacteria on various media and examine the roles of external and internal factors in gene regulation. Gene expression in all organisms is carefully regulated to allow adaptation to differing conditions and to prevent wasteful production of proteins. Bacterial genes encoding the enzymes needed to metabolize the simple sugar arabinose are a perfect example. A promoter region upstream of these genes acts as a molecular on/off switch that regulates their expression. The genes are activated only when arabinose is present in the environment. Bio-Rad's pGLO plasmid incorporates the arabinose promoter, but the genes involved in the breakdown of arabinose have been replaced with the jellyfish gene encoding GFP. When bacteria transformed with pGLO plasmid are grown in the presence of arabinose, the GFP gene switches on, causing the bacteria to express GFP and fluoresce brilliant green. When students genetically reengineer bacteria with the genes from a bioluminescent jellyfish, they never forget the central mantra of molecular biology:

DNA ⇒ RNA ⇒ Protein ⇒ Trait — Green Fluorescence

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Plasmid (pGLO), lyophilized 1
E. coli strain HB101 K-12, lyophilized 1
LB nutrient broth, sterile 1
LB nutrient agar powder 1
Ampicillin, lyophilized 1
Arabinose, lyophilized 1
Sterile transformation solution (CaCl2) 1
Petri dishes, 60 mm, sterile 40
Inoculation loops, sterile 80
Microcentrifuge tubes, 2.0 ml, sterile, color-coded 60
Foam floats 8
Disposable plastic transfer pipets 50
UV pen light 1
pGLO™ Bacterial Transformation Kit

166-0003EDU
Gene expression analysis classroom kit, includes GFP encoding plasmid, nutrient broth/powder, petri dishes, UV pen light, and more, for 32 students; education use only, download free manual

List Price:   $117.50
EDU Price:   $94.00   What is this?
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Number Description Options
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge, Rev A Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories, Rev A Click to download
6605 Map of AP-Aligned Bio-Rad Kits with Learning Objectives, Rev B Click to download
6581 20 Questions to Master Inquiry - pGLO™ Bacterial Transformation Kit, Rev A Click to download
3052 pGLO Bacterial Transformation Kit, Biotechnology Explorer, Quick Guide Click to download
LIT1660033EDU Instruction Manual, pGLO Bacterial Transformation Kit, Biotechnology Explorer, Rev G Please login to download this document
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide, Rev A Click to download
5872 Biotechnology Explorer Refresh Kit Components Purchasing Guide, Rev A Click to download
M1660023 Instruction Manual, Protein Electrophoresis of GFP: A pGLO Bacterial Transformation Kit Extension, Biotechnology Explorer, Rev D Please login to download this document