Personal Molecular Imager™ (PMI) System

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Overview

Laser Scanner Selection Guide
Applications and Features PharosFX™ PharosFX Plus PMI™
Fluorescence
    Blue-excited
    (488 nm external laser)
O O -
    Green-excited
    (532 nm internal laser)
S S -
    Red-excited
    (635 nm external laser)
O O -
    Multiplex applications S S -
Radioisotope detection (Kodak/
Fuji screens) using internal laser
- S
(532 nm)
S
(635 nm)
Choice of emission filters
(including custom filters)
S S -
USB2 Interface S S S

S - Standard
O - Optional
- Not available

Comparison of PharosFX and PMI Systems
    Model Characteristics
    PharosFX Plus PharosFX PMI
Detection limit
    Storage phosphor <0.95 dpm/mm2 for one exposure to 14C using imaging screen-K X   X
<0.15 dpm/mm2 for one exposure to 32P using imaging screen-K X   X
    Fluorescence
    (depends on
    experimental
    conditions)
0.2 fmol of FITC end-labeled DNA using 488 nm laser X X  
6 pg of SYBR® Green I-stained DNA using 488 nm laser X X  
0.4 fmol of FITC end-labeled DNA using 532 nm laser X X  
25 pg of SYBR® Green I-stained DNA using 532 nm laser X X  
0.2 fmol of Cy3 end-labeled DNA using 532 nm laser X X  
0.2 fmol Cy5 end-labeled DNA using 635 nm laser X X  
Dynamic Range 5 orders of magnitude (1:65,535) or 16-bit X X X
Linearity r2 > 0.99 X X X
Uniformity ±5% over entire scan area X X X
Scan resolution 800, 200, 200 and 50 µm (user selectable) X X X
Scan time 20 x 25 cm area; 8.5 min at 100 µm, 15 min at 50 µm X X X
35 x 43 cm area; 8.5 min at 200 µm, 17 min at 100 µm X X X
Spatial resolution of storage phosphor* 14C:200 µm (2.5 line pairs/mm) using imaging screen-K X   X
32P:300 µm (1.5 line pairs/mm) using imaging screen-K X   X
Digital resolution 16-bit (65,636 gray scale) X X X
Excitation source 25 mW 532 nm (green) diode-pumped solid-state laser X X  
10 mW 635 nm diode laser X   X
Optional external lasers 15 mW 488 nm (blue) extermal argon ion laser X X  
10 mW 635 nm (red) extermal diode laser X X  
Maximum power 65 W X X  
Input voltage range 100–240 VAC, 50–60 Hz X X X
Operating humidity requirements 10–32°C, 30–80% X X X
Computer interface USB2 X X X
Operating system Windows 2000, XP, Vista or Mac OS X X X X
Dimensions
(W x D X H)
57 x 66 x 30 cm (22.4 x 26.8 x 11.8") X X X
Weight 32 kg (70.5 lb; scanner) X X X

*Dependent on radioisotope characteristics and storage phosphor crystal size coated on the screen.

Images Acquired with PMI

Left, 14C-labeled rat; center, 35S-labeled sequencing gel; right, 14S-labeled 2-D PAGE gel.

Imaging Screen Specifications and Recommended Applications
Screen Name Application Key Features Sizes (W x H) Catalog #
Imaging screen-K

32P, 33P, 14C, 35S

BaFBr; Eu formulation 35 x 43 cm 170-7841
    Protective coating    
    Easy-to-use format    
    Compatible with standard X-ray cassettes 20 x 25 cm 170-7843
Imaging screen-K/tritium

3H

BaFBr:Eu formulation 20 x 25 cm 170-7845
    Sensitive to weak 3H signal    
    Easy-to-use format    
    Compatible with standard X-ray cassettes    

The Personal Molecular Imager™ system is the most flexible isotope imaging and laser scanning system available, offering radioisotopic imaging using your choice of storage phosphor screen. The PMI™ system accommodates both large (35 x 43 cm) and small (20 x 25 cm) storage phosphor screens, as well as a wide range of storage phosphor screen types for the quantitation and imaging of 32P, 33P, 35S, 14C, and 3H. It supports the use of Kodak (BaFBr:Eu) screens, which can be used with standard autoradiography cassettes. The system is also compatible with screens from other suppliers.

Storage phosphor technology offers the ultimate in isotopic sensitivity, with exposure times typically one-tenth that of film. In addition, its quantitative accuracy is far superior.

Linear dynamic range 5 orders of magnitude (1 to 100,000)
Selectable resolution 50, 100, 200, and 800 µm
Pixel density 16-bit (65,536 gray levels)
Uniformity ±5% over entire area
Excitation source 635 nm diode laser
Detector 1 PMT
Maximum imaging area 35 x 43 cm
Maximum sample area 35 x 43 cm
Weight 31 kg (68.3 lb)
System dimensions (W x D x H) 57 x 69 x 30 cm
Personal Molecular Imager™ (PMI™) System

170-9400
100/240 V, PC or Mac, includes Quantity One® software, sample tray, USB 2 cable

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Number Description Options
5475 Personal Molecular Imager (PMI) Folder Insert, Rev B Click to download
6126 Imaging Systems, Computer Specifications, Rev D Click to download
5888 Bio-Rad Imaging Systems Family Brochure, Rev E Click to download [ Add to Cart (Free) ]
4000111 Instruction Manual, Personal Molecular Imager FX, Rev F Click to download
5331 Molecular Imager PharosFX Systems Brochure, Rev B Click to download [ Add to Cart (Free) ]
2318 Proteomics in Bladder Cancer Research: Protein Profiling of Bladder Squamous Cell Carcinomas, Rev C Click to download
3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download [ Add to Cart (Free) ]