PCR Amplification Reagents

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Additional Product Descriptions

iTaq™ DNA Polymerase
iTaq DNA polymerase is an antibody-mediated hot-start DNA polymerase suitable for both conventional and real-time PCR applications and ensures ease of use, high specificity, and sensitivity.

2x Master Mix for PCR
2x Master Mix for PCR is a concentrated solution of Taq DNA Polymerase, dNTPs, and all the components required for PCR, except DNA template and primers. This premixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR setup. The mix is optimized for efficient and reproducible PCR.

The absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.

dNTP Mix
This dNTP mix is formulated for optimal performance in real-time PCR applications and is also qualified for use in conventional PCR applications providing consistent, high-yield amplification.

SYBR® Green
iQ SYBR® Green supermix is formulated for optimal results in real-time PCR based on SYBR® Green I detection. It contains SYBR® Green I dye, hot-start iTaq DNA polymerase, optimized buffer, and dNTPs qualified for quantitative PCR. Alternately, Bio-Rad's iScript™ one-step RT-PCR kit with SYBR® Green allows cDNA synthesis and PCR to be conveniently carried out in the same tube.

iTaq DNA Polymerase

iTaq DNA polymerase outperforms a chemically modified hot-start DNA polymerase over a wide range of targets. Human genomic DNA fragments from 300 bp to 4 kb were amplified with both polymerases. Lane M, EZ Load 500 bp molecular ruler.

iQ SYBR® Green Supermix

iQ SYBR® Green supermix and iScript cDNA synthesis kit show high specificity over a broad dynamic range of cDNA. Serial dilutions (1 µg to 1 pg) of HeLa total RNA were reverse transcribed, and the resulting cDNA amplified using primers specific to the β-actin gene. Triplicate reactions were amplified along with no-template controls on the iCycler iQ real-time system. The consistent spacing of the curves reflects accurate reverse transcription and amplification. Standard curve had r = 0.997, slope = –3.615, efficiency = 89.1%.

iQ SYBR® Green supermix generates precise, quantitative results. A 5-fold dilution series (50 ng–80 pg) of human genomic DNA was amplified using the supermix and primers and a probe specific to the IL-1β gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ real-time system. Standard curve had r = 0.999, slope = –3.380, efficiency = 97.6%.

iQ SYBR® Green supermix shows consistently high specificity with plasmid templates. Ten-fold serial dilutions (108 to 102 copies) of plasmid DNA were amplified using primers specific to the β-actin gene. Triplicate reactions at each concentration were amplified along with no-template controls on the iCycler iQ real-time system. Standard curve had r = 1.000, slope = –3.492, efficiency = 93.4%.

iScript One-Step RT-PCR Kit

The iScript one-step RT-PCR kit with SYBR® Green provides high sensitivity across a broad range of concentrations. One-step RT-PCR reations were performed in quadruplicate, along with no-template controls, using β-actin primers and 100 ng-1 pg of Hela total RNA. Reactions were carried out on the MyiQ real-time system. Standard curve had r=0.999, slope=-3.422, efficiency=96%.

Bio-Rad's reagents are specially formulated to meet both conventional and real-time PCR requirements with minimal need for optimization. All reagents are qualified for use on Bio-Rad's complete line of thermal cyclers and real-time PCR detection systems, and demonstrate high performance for cDNA, genomic DNA, and plasmid DNA amplification over a wide dynamic range.

iTaq™ DNA Polymerase

iTaq DNA polymerase is an antibody-mediated hot-start DNA polymerase suitable for both conventional and real-time PCR applications that ensures ease of use and high specificity and sensitivity.

2x Master Mix for PCR

Bio-Rad's 2x master mix for PCR is a concentrated solution of Taq DNA polymerase, dNTPs, and all the components required for PCR, except the DNA template and primers. This premixed formulation saves time and reduces contamination by reducing the number of pipetting steps required for PCR setup. The mix is optimized for efficient and reproducible PCR.

The absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases is confirmed by appropriate quality tests. The master mix is functionally tested in amplification of a single-copy gene from human genomic DNA.

dNTP Mix

This dNTP mix is formulated for optimal performance in real-time PCR applications and is also qualified for use in conventional PCR applications. It provides consistent, high-yield amplification.

SYBR® Green Supermix

iQ™ SYBR Green supermix is formulated for optimal results in real-time PCR based on SYBR Green I detection. It contains SYBR Green I dye, hot-start iTaq DNA polymerase, optimized buffer, and dNTPs qualified for quantitative PCR. Alternately, Bio-Rad's iScript™ one-step RT-PCR kit with SYBR Green allows cDNA synthesis and PCR to be conveniently carried out in the same tube.

Chill-Out Liquid Wax

CHO-1411EDU
100 ml, vapor barrier for use in thermal cyclers, clear, optical grade; education use only

List Price:   $51.00
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dNTP Mix

170-8874EDU
200 μl, premixed dNTP solution, 10 mM each (dATP, dCTP, dGTP, dTTP); education use only

List Price:   $120.00
EDU Price:   $96.00   What is this?
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iQ SYBR® Green Supermix

170-8880EDU
100 x 50 µl reactions, 2x real-time PCR mix contains 100 mM KCl, 40 mM Tris-HCl, pH 8.4, 0.4 mM each dNTP, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2, SYBR® Green I, 20 nM fluorescein; education use only

List Price:   $183.00
EDU Price:   $146.40   What is this?
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iScript™ One-Step RT-PCR Kit with SYBR® Green

170-8892EDU
50 x 50 μl reactions, one-step real time RT-PCR kit, includes iScript reverse transcriptase, 2x SYBR® Green RT-PCR reaction mix, nuclease-free water; education use only

List Price:   $189.00
EDU Price:   $151.20   What is this?
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iTaq™ DNA Polymerase

170-8870EDU
5 U/μl, 250 U hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2 solution; education use only

List Price:   $225.00
EDU Price:   $180.00   What is this?
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Master Mix For PCR

166-5009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only

List Price:   $92.50
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MgCl2 Solution

170-8872EDU
1.25 ml, 50 mM MgCl2 solution, for PCR applications; education use only

List Price:   $26.00
EDU Price:   $20.80   What is this?
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Number Description Options
5458 Gene Expression Workflow Brochure, Rev B Click to download
2779 iTaq DNA Polymerase Flier, Rev E Click to download [ Add to Cart (Free) ]
2894 iScript cDNA Synthesis Kits Flier, Rev F Click to download
4106269 Instruction Manual, iScript One-Step RT-PCR Kit with SYBR Green, Rev E Click to download
5345 Reagents for Reverse Transcription, PCR, and Real-Time PCR Brochure, Rev C Click to download
2764 iQ Supermixes for qPCR, Rev E Click to download
4106212 Instruction Manual, iQ SYBR Green Supermix, 100 x 50 µl, Rev D Click to download [ Add to Cart (Free) ]